Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermobifida fusca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 125% activity at 1 mM | Thermobifida fusca | |
Mn2+ | 119% activity at 1 mM | Thermobifida fusca | |
additional information | not influenced by K+, Na+, Mg2+, and Zn2+ | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | Q47SE9 | - |
- |
Thermobifida fusca ATCC BAA-629 | Q47SE9 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-charged his-affinity gel chromatography | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | highest activity | Thermobifida fusca | ? | - |
? | |
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | highest activity | Thermobifida fusca ATCC BAA-629 | ? | - |
? | |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Thermobifida fusca | ? | - |
? | |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Thermobifida fusca ATCC BAA-629 | ? | - |
? | |
colloidal chitin + H2O | low activity | Thermobifida fusca | ? | - |
? | |
colloidal chitin + H2O | low activity | Thermobifida fusca ATCC BAA-629 | ? | - |
? | |
additional information | the enzyme shows no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide | Thermobifida fusca | ? | - |
? | |
additional information | the enzyme shows no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide | Thermobifida fusca ATCC BAA-629 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44900, SDS-PAGE | Thermobifida fusca |
Synonyms | Comment | Organism |
---|---|---|
endochitinase | - |
Thermobifida fusca |
Tfu_0580 | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Thermobifida fusca |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 65 | the enzyme retains more than 80% activity between 20 and 55°C, while the chitinase activity drops to less than 40% when temperature is above 65°C | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermobifida fusca |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 7.5 | the enzyme tends to be active under an acidic condition, which remain more than 60% highest activity between pH 4.8 and 7.5. When pH is at 8.0, the enzyme activity drops to 40% of its highest activity | Thermobifida fusca |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermobifida fusca | calculated from amino acid sequence | - |
5.8 |