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Literature summary for 3.1.4.53 extracted from
- Characterization of cyclic AMP phosphodiesterases in Leishmania mexicana and purification of a soluble form (2000), Mol. Biochem. Parasitol., 106, 283-292.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 0.3 mM, more than 95% inhibition | Leishmania mexicana |  |
| 3-isobutyl-1-methyl-xanthine | - |
Leishmania mexicana | |
| Ca2+ | - |
Leishmania mexicana | |
| Caffeine | 50 mM, 30% inhibition of soluble enzyme | Leishmania mexicana | |
| Cibacron blue | - |
Leishmania mexicana | |
| EDTA | 0.3 mM, more than 95% inhibition | Leishmania mexicana | |
| OPC-3911 | - |
Leishmania mexicana | |
| rolipram | - |
Leishmania mexicana | |
| theophylline | - |
Leishmania mexicana | |
| zaprinast | - |
Leishmania mexicana | |
| Zn2+ | - |
Leishmania mexicana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.277 | - |
adenosine 3',5'-cyclic phosphate | 30°C | Leishmania mexicana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Leishmania mexicana | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates | Leishmania mexicana | |
| Mn2+ | stimulates | Leishmania mexicana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
x * 61000, SDS-PAGE | Leishmania mexicana |
| 200000 | - |
gel filtration | Leishmania mexicana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania mexicana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leishmania mexicana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| promastigote | - |
Leishmania mexicana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.02689 | - |
- |
Leishmania mexicana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine 3',5'-cyclic phosphate + H2O | the enzyme is highly specific for cAMP | Leishmania mexicana | adenosine 5'-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, SDS-PAGE | Leishmania mexicana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Leishmania mexicana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Leishmania mexicana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 9.2 | pH 6.3: about 40% of maximal activity, pH 9.2: about 80% of maximal activity | Leishmania mexicana |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
soluble enzyme | Leishmania mexicana | Cibacron blue | |
| 0.02 | - |
particulate enzyme | Leishmania mexicana | Cibacron blue | |
| 0.04 | - |
particulate enzyme | Leishmania mexicana | zaprinast | |
| 0.3 | - |
soluble enzyme | Leishmania mexicana | OPC-3911 | |
| 0.6 | - |
particulate enzyme | Leishmania mexicana | rolipram | |
| 0.7 | - |
soluble enzyme | Leishmania mexicana | rolipram | |
| 0.8 | - |
particulate enzyme | Leishmania mexicana | OPC-3911 | |
| 2 | - |
soluble enzyme | Leishmania mexicana | 3-isobutyl-1-methyl-xanthine | |
| 2.8 | - |
soluble enzyme | Leishmania mexicana | zaprinast | |
| 3 | - |
particulate enzyme | Leishmania mexicana | theophylline | |
| 3 | - |
particulate enzyme | Leishmania mexicana | 3-isobutyl-1-methyl-xanthine | |
| 19 | - |
particulate enzyme | Leishmania mexicana | Caffeine | |
| 30 | - |
soluble enzyme | Leishmania mexicana | theophylline | |
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