Cloned (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
General Stability | Organism |
---|---|
stable | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PO43- | competitive | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
p-nitrophenyl 5'-thymidine monophosphate | +/- 0.003, with 1 mM Ni2+ | Methanocaldococcus jannaschii | |
0.15 | - |
bis-p-nitrophenyl phosphate | +/- 0.01, with 1 mM Ni2+ | Methanocaldococcus jannaschii | |
0.66 | - |
bis-p-nitrophenyl phosphate | +/- 0.09, with 20 mM PO43- and 1 mM Ni2+ | Methanocaldococcus jannaschii | |
1.01 | - |
bis-p-nitrophenyl phosphate | +/- 0.13, with 50 mM PO43- and 1 mM Ni2+ | Methanocaldococcus jannaschii | |
1.53 | - |
p-Nitrophenylphosphorylcholine | +/- 0.1, with 20 mM Mn2+ | Methanocaldococcus jannaschii | |
3.33 | - |
p-Nitrophenylphosphorylcholine | +/- 0.5, with 1 mM Ni2+ | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates, KD: 1.3 +/- 0.1 uM | Methanocaldococcus jannaschii | |
Ni2+ | activates, KD: 0.28 +/- 0.09 mM | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58346 | - |
- |
Storage Stability | Organism |
---|---|
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bis-p-nitrophenyl phosphate + H2O | - |
Methanocaldococcus jannaschii | p-nitrophenol + p-nitrophenyl phosphate | - |
? | |
p-nitrophenyl 5'-thymidine monophosphate + H2O | - |
Methanocaldococcus jannaschii | 5'-TMP + p-nitrophenol | - |
? | |
p-nitrophenylphosphorylcholine + H2O | - |
Methanocaldococcus jannaschii | p-nitrophenol + phosphorylcholine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | crystallization | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
phosphodiesterase | - |
Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.26 | - |
p-nitrophenyl 5'-thymidine monophosphate | +/- 0.006, with 1mM Ni2+ | Methanocaldococcus jannaschii | |
8.6 | - |
p-Nitrophenylphosphorylcholine | +/- 0.5, with 1 mM Ni2+ | Methanocaldococcus jannaschii | |
52.9 | - |
p-Nitrophenylphosphorylcholine | +/- 1.0, with 20 mM Mn2+ | Methanocaldococcus jannaschii | |
105.7 | - |
bis-p-nitrophenyl phosphate | +/- 2.8, with 1 mM Ni2+ | Methanocaldococcus jannaschii | |
125.9 | - |
bis-p-nitrophenyl phosphate | +/- 8.1, with 20 mM PO43- and 1 mM Ni2+ | Methanocaldococcus jannaschii | |
130.2 | - |
bis-p-nitrophenyl phosphate | +/- 8.6, with 50 mM PO43- and 1 mM Ni2+ | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | 9.8 | - |
Methanocaldococcus jannaschii |