Inhibitors | Comment | Organism | Structure |
---|---|---|---|
metavanadate | 50% inhibition at 0.0011 and pH 2.5, or at 0.0053 mM and pH 5.0 | Aspergillus niger | |
metavanadate | 50% inhibition at 0.00075 mM and pH 5.0 or at 0.00090 mM and pH 3.0 | Escherichia coli | |
orthovanadate | 50% inhibition at 0.0004 and pH 2.5, or at 0.040 mM and pH 5.0 | Aspergillus niger | |
orthovanadate | 50% inhibition at 0.0022 mM and pH 5.0 or at 0.000072 mM and pH 3.0 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | 0.324 | phytate | pH 2.5, 55°C | Aspergillus niger | |
0.25 | 0.425 | phytate | pH 4.0, 55°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phytate + H2O | Aspergillus niger | - |
? + phosphate | - |
? | |
phytate + H2O | Escherichia coli | - |
? + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
gene phyA | - |
Escherichia coli | Q6RK08 | gene AppA2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phytate + H2O | - |
Aspergillus niger | ? + phosphate | - |
? | |
phytate + H2O | - |
Escherichia coli | ? + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AppA2 | - |
Escherichia coli |
histidine acid phosphatase | - |
Aspergillus niger |
histidine acid phosphatase | - |
Escherichia coli |
PhyA | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Aspergillus niger |
55 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1260 | - |
phytate | pH 2.5, 55°C | Aspergillus niger | |
2348 | 4470 | phytate | pH 4.0, 55°C | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 5 | - |
Aspergillus niger |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | vanadate inhibition kinetics | Aspergillus niger | |
additional information | - |
additional information | vanadate inhibition kinetics | Escherichia coli | |
0.0002 | - |
orthovanadate | pH 4.0, 55°C, 0.0005 mM orthovanadate | Escherichia coli | |
0.004 | - |
metavanadate | pH 4.0, 55°C, 0.010 mM metavanadate | Escherichia coli | |
0.0056 | - |
metavanadate | pH 2.5, 55°C, 0.015 mM metavanadate | Aspergillus niger | |
0.0057 | - |
orthovanadate | pH 2.5, 55°C, 0.010 mM orthovanadate | Aspergillus niger |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme can also act as a vanadate haloperoxidase, EC 1.11.1.10 | Aspergillus niger |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
phytate | pH 2.5, 55°C | Aspergillus niger | |
0.0093 | 0.01 | phytate | pH 4.0, 55°C | Escherichia coli |