Application | Comment | Organism |
---|---|---|
additional information | important role for TRM2 in DNA repair with a potential involvement of its nuclease function in homologous recombination based repair of DNA double-strand breaks, plays no role in the nucleotide excision repair and/or base excision repair pathways | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
Escherichia coli BL21-Gold DE3 transformed with the plasmid pCBP-TRM2 and pCBP-DTRM2(ctdelta1689-1920nt), also cloned into a GFP vector bearing a GAL1::GFP transcriptional fusion | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Trm2p(ctdelta76aa), lacks 76 amino acids at the C-terminus, retains nuclease activities but not the methyl transferase activity. Trm2(delta232-1920nt) mutant, contains only the first 231 nucleotides of the TRM2 gene, displays low sensitivity to methyl methane sulfonate and suppresses the methyl methane sulfonate sensitivity of rad52 mutants in trm2(delta232-1920nt)rad52 double mutants. Trm2 exo1 double mutants are synergistically more sensitive to methyl methane sulfonate and ionizing radiation than either of the single mutant. The ku80trm2(deltaD232-1920nt) double mutant displays much higher methyl methane sulfonate sensitivity than either of the single mutant alone. The trm2 (delta232-1920nt) single mutant conferrs only a very slight sensitivity to gamma irradiation, while the trm2(delta232-1920nt)exo1 double mutant exhibits 1.7-2.2fold higher sensitivity relative to trm2(delta232-1920nt) mutant | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pentamidine | endo-exonuclease inhibitor, inhibits DNase activity of CBP-Trm2p | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by calmodulin affinity chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Trm2p displays 5' to 3' exonuclease activity on double-strand DNA, and endonuclease activity on single-strand DNA | Saccharomyces cerevisiae | deoxyribonucleoside 3'-phosphates | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trm2p | - |
Saccharomyces cerevisiae |