Application | Comment | Organism |
---|---|---|
analysis | end group and sequential analysis of polynucleotides | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
no contamination by other phosphohydrolase activities | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phospho-2'-deoxyribooligonucleotide + H2O | average size 10-12, smallest 3 | Sus scrofa | 2'-deoxyribonucleoside 3'-phosphate | - |
? | |
4-nitrophenyl phosphate | - |
Sus scrofa | 4-nitrophenol + phosphate | - |
? | |
5'-hydroxy oligonucleotides + H2O | dependency on type of nucleobase | Sus scrofa | nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates | - |
? | |
bis-(4-nitrophenyl)phosphate + H2O | - |
Sus scrofa | 4-nitrophenyl phosphate + 4-nitrophenol | - |
? | |
DNA + H2O | alkali-denaturated DNA, 25 times lower degradation rate with native DNA | Sus scrofa | deoxyribonucleoside 3'-phosphates | - |
? | |
polyadenylic acid + H2O | - |
Sus scrofa | 5'-hydroxy-adenine oligonucleotides + 3'AMP | - |
? | |
polyinosinic acid | - |
Sus scrofa | 3'-IMP | - |
? | |
polyuridylic acid + H2O | - |
Sus scrofa | 3'-UMP | - |
? | |
RNA core + H2O | RNA core are water undializable 3'-phosphoribooligonucleotides obtained by exhaustive digestion of RNA with pancreatic ribonuclease | Sus scrofa | nucleoside 3'-phosphates | - |
? | |
thymidine 3'-(4-nitrophenyl)phosphate + H2O | very slow hydrolysis | Sus scrofa | thymidine 3'-phosphate + 4-nitrophenol | - |
? | |
tRNA + H2O | from Escherichia coli | Sus scrofa | tRNA fragments + nucleoside 3'-phosphates | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphodiesterase II | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 80 | range of thermal inactivation, 50% of activity at 56°C | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
with 0.1 M acetate or succinate buffer | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | poor effect of 0.02 M Mg2+ on pH activity curve at 37°C | Sus scrofa |