Application | Comment | Organism |
---|---|---|
analysis | kinetic studies on macromolecular substrates degradation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | after 2 h incubation almost 80% of activity with 1.0 M, 40% with 2.0 M, no activity with 5.0 M | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
molecular sieving, SDS gel electrophoresis, sucrose density gradient centrifugation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
no endonuclease and phophomonoesterase activities | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
4°C, lyophilized crude enzyme, more than 1 year, no loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA core + H2O | - |
Bos taurus | nucleoside 3'-phosphates | - |
? | |
thymidine 3'-(4-nitrophenyl)phosphate + H2O | - |
Bos taurus | thymidine 3'-phosphate + 4-nitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphodiesterase II | - |
Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | incubation at pH 5.7, 10 min: 90% of initial activity at 35°C, 10% at 60°C | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | incubation of pure enzyme for 2 h at 4°C, beyond the limits sharp decrease of stability | Bos taurus |