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Literature summary for 3.1.1.64 extracted from

  • Yuan, Q.; Kaylor, J.J.; Miu, A.; Bassilian, S.; Whitelegge, J.P.; Travis, G.H.
    Rpe65 isomerase associates with membranes through an electrostatic interaction with acidic phospholipid headgroups (2010), J. Biol. Chem., 285, 988-999.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.64

Activating Compound

Activating Compound Comment Organism Structure
additional information Rpe65 isomerase activity is strongly stimulated by addition of either 10% phosphatidylglycerol (10.5fold increase of activity) or 10% phosphatidylserine (8.5fold increase of activity) to liposomes containing the same major phospholipids as RPE membranes Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Gallus gallus 16020
-
membrane Rpe65 is strongly associated with membranes but contains no membrane-spanning segments Bos taurus 16020
-

Organism

Organism UniProt Comment Textmining
Bos taurus Q28175
-
-
Gallus gallus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification Rpe65 is not palmitoylated nor does it appear to undergo other post-translational modifications at significant stoichiometry Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
retinal pigment epithelial cell
-
 Gallus gallus
-
retinal pigment epithelial cell
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinyl palmitate + H2O
-
 Gallus gallus 11-cis-retinol + palmitate
-
 ?
all-trans-retinyl palmitate + H2O
-
 Bos taurus 11-cis-retinol + palmitate
-
 ?
additional information binding of Rpe65 to liposomes containing phosphatidylserine or phosphatidylglycerol, but not the basic or neutral phospholipids, allows the enzyme to extract its insoluble substrate, all-trans-retinyl palmitate, from the lipid bilayer for synthesis of 11-cis-retinol Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
Rpe65
-
 Gallus gallus
Rpe65
-
 Bos taurus