Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, complexed with L-propargylglycine | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H123A | decreased specific activity towards L-selenocysteine | Escherichia coli |
H55A | normal activity towards L-selenocysteine and L-cysteine | Escherichia coli |
R379A | significant loss of activity towards L-selenocysteine | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + [enzyme]-cysteine | Escherichia coli | - |
L-alanine + [enzyme]-S-sulfanylcysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor | mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
H123 mutant with L-selenocysteine as substrate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + [enzyme]-cysteine | - |
Escherichia coli | L-alanine + [enzyme]-S-sulfanylcysteine | - |
? | |
L-cysteine + [enzyme]-cysteine | Cys364 residue is essential for activity toward L-cysteine but not toward L-selenocyteine | Escherichia coli | L-alanine + [enzyme]-S-sulfanylcysteine | - |
? | |
L-selenocysteine | Cys364 residue is essential for activity toward L-cysteine but not toward L-selenocyteine | Escherichia coli | L-alanine + selenium | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |