Crystallization (Comment) | Organism |
---|---|
structure in the presence of 3'-dATP and Mn2+. The metal ion is octahedrally coordinated with all six coordination positions occupied by oxygen ligands. The three phosphate groups from 3'-dATP together with Asp75 from the adjacent subunit and two water molecules are all located between 2.0 and 2.5 A from the manganese. The pre-reaction state shows a highly coordinated arrangement of the two ATP (-analogues) in almost optimal distance for the nucleophilic attack of the 3'-OH on the alpha-phosphate of the neighbouring ATP. The rate-limiting step of cyclic di-AMP synthesis by DisA is not defined by the reaction itself, but rather by the accessibility of the active site | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
D75N | inactive | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-dATP | competitive inhibitor of the diadenylate cyclase domain, binds in the same position as ATP | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
- |
Thermotoga maritima |
339000 | - |
gel filtration, mutant D75N | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WY43 | - |
- |
Thermotoga maritima DSM 3109 | Q9WY43 | - |
- |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 42000, calculated | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
disA | - |
Thermotoga maritima |
DNA integrity scanning protein | - |
Thermotoga maritima |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.003 | - |
pH 9.5, 60°C | Thermotoga maritima | 3'-dATP |