Protein Variants | Comment | Organism |
---|---|---|
E92F | the mutant is nearly inactive for CCA addition, although tRNA binding is apparently normal | Saccharolobus shibatae |
G166R | C74-adding is 7% of wild-type activity, C75-adding is 8% of wild-type activity, A76-adding is 21% of wild-type activity | Saccharolobus shibatae |
G169R | C74-adding is 5% of wild-type activity, C75-adding is 2% of wild-type activity, A76-adding is 22% of wild-type activity | Saccharolobus shibatae |
H129A | C74-adding is 22% of wild-type activity, C75-adding is 28% of wild-type activity, A76-adding is 87% of wild-type activity | Saccharolobus shibatae |
H93V | the mutant adds C74 and C75 but fails to add A76 | Saccharolobus shibatae |
H93V/Y95V | the mutant enzyme is completely inactive for CCA addition | Saccharolobus shibatae |
K149A | no C74-adding activity, C75-adding is 1% of wild-type activity, A76-adding is 1% of wild-type activity | Saccharolobus shibatae |
K153A | C74-adding is 14% of wild-type activity, C75-adding is 4% of wild-type activity, A76-adding is 3% of wild-type activity | Saccharolobus shibatae |
additional information | mutations near the active site can differentially affect addition of C74, C75, or A76 | Saccharolobus shibatae |
R125A | C74-adding is 30% of wild-type activity, C75-adding is 10% of wild-type activity, A76-adding is 5% of wild-type activity | Saccharolobus shibatae |
Y158A | C74-adding is 9% of wild-type activity, C75-adding is 15% of wild-type activity, A76-adding is 7% of wild-type activity | Saccharolobus shibatae |
Y90E | the mutant is nearly wild type for CCA addition despite replacement of a conserved bulky hydrophobic residue by a carboxylate | Saccharolobus shibatae |
Y90E/E92F | the mutant enzyme is completely inactive for CCA addition | Saccharolobus shibatae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
CTP | pH 9.0, 70°C, wild-type enzyme | Saccharolobus shibatae | |
0.02 | - |
CTP | pH 9.0, 70°C, mutant enzyme R125A | Saccharolobus shibatae | |
0.03 | - |
CTP | pH 9.0, 70°C, mutant enzyme E92F | Saccharolobus shibatae | |
0.033 | - |
CTP | pH 9.0, 70°C, mutant enzyme Y158A | Saccharolobus shibatae | |
0.04 | - |
CTP | pH 9.0, 70°C, mutant enzyme H129A | Saccharolobus shibatae | |
0.057 | - |
CTP | pH 9.0, 70°C, mutant enzyme K153A | Saccharolobus shibatae | |
0.062 | - |
CTP | pH 9.0, 70°C, mutant enzyme Y90E/E92F | Saccharolobus shibatae | |
0.067 | - |
CTP | pH 9.0, 70°C, mutant enzyme H93V/Y95V | Saccharolobus shibatae | |
0.1 | - |
CTP | pH 9.0, 70°C, mutant enzyme G166R | Saccharolobus shibatae | |
0.142 | - |
CTP | pH 9.0, 70°C, mutant enzyme G169R | Saccharolobus shibatae | |
0.167 | - |
CTP | pH 9.0, 70°C, mutant enzyme K149A | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | P77978 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a tRNA precursor + 2 CTP + ATP | overall reaction | Saccharolobus shibatae | a tRNA with a 3' CCA end + 3 diphosphate | - |
? | |
a tRNA precursor + CTP | - |
Saccharolobus shibatae | a tRNA with a 3' cytidine end + diphosphate | - |
? | |
a tRNA with a 3' CC end + ATP | - |
Saccharolobus shibatae | a tRNA with a 3' CCA end + diphosphate | - |
? | |
a tRNA with a 3' cytidine + CTP | - |
Saccharolobus shibatae | a tRNA with a 3' CC end + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCA-adding enzyme | - |
Saccharolobus shibatae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus shibatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Saccharolobus shibatae |