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Literature summary for 2.7.7.72 extracted from

  • Yakunin, A.F.; Proudfoot, M.; Kuznetsova, E.; Savchenko, A.; Brown, G.; Arrowsmith, C.H.; Edwards, A.M.
    The HD domain of the E. coli tRNA nucleotidyltransferase has 2',3'-cyclic phosphodiesterase, 2'-nucleotidase, and phosphatase activities (2004), J. Biol. Chem., 279, 36819-36827.
    View publication on PubMed
Search for more literature for 2.7.7.72

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the isolated His-tagged HD domain in Escherichia coli strain BL21 (DE3) Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the HD domain of the enzyme also exhibits 2',3'-cyclic phosphodiesterase, 2'-nucleotidase, and Ni2+-dependent phosphatase activities, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P06961
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged HD domain from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the HD domain of the enzyme also exhibits 2',3'-cyclic phosphodiesterase, 2'-nucleotidase, and Ni2+-dependent phosphatase activities, overview Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme comprises two domains: an N-terminal domain containing the nucleotidyltransferase activity and a C-terminal HD domain Escherichia coli

Synonyms

Synonyms Comment Organism
ATP(CTP):tRNA nucleotidyltransferase
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 Escherichia coli
tRNA nucleotidyltransferase
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 Escherichia coli
tRNA-NT
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 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

General Information

General Information Comment Organism
physiological function in all mature tRNAs, the 3'-terminal CCA sequence is synthesized or repaired by the template-independent nucleotidyltransferase ATP(CTP):tRNA nucleotidyltransferase. The phosphohydrolase activities of the HD domain of the tRNA nucleotidyltransferase are involved in the repair of the 3'-CCA end of tRNA, modeling, overview Escherichia coli