Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 10% inhibition | Escherichia coli | |
Co2+ | 1 mM, 40% inhibition | Escherichia coli | |
Cu2+ | 0.1 mM, 92% inhibition, 1 mM, complete inhibition | Escherichia coli | |
Mn2+ | weak inhibition at 10 mM Mg2+ | Escherichia coli | |
Zn2+ | 0.1 mM, 67% inhibition, 1 mM, complete inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
ADP | pH 5.5, 25°C | Escherichia coli | |
0.18 | - |
ADP | pH 6.5-8.5, 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1.5-2.5fold lower activation than with Mg2+, inhibitory with Mg2+ as activator | Escherichia coli | |
Mg2+ | activation | Escherichia coli | |
Mg2+ | maximal activity at 10 mM | Escherichia coli | |
Mn2+ | activation, can replace Mg2+ | Escherichia coli | |
Mn2+ | 1.5-2.5fold lower activation than with Mg2+ | Escherichia coli | |
additional information | not activated by Ca2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (phosphate)n+1 | - |
Escherichia coli | ATP + (phosphate)n | - |
r | |
ATP + (phosphate)n | best substrates are polyphosphates with more than 132 residues | Escherichia coli | ADP + (phosphate)n+1 | - |
r | |
ATP + (phosphate)n | no activity with phosphates of 5 residues or below | Escherichia coli | ADP + (phosphate)n+1 | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | approx. 19% of maximal activity at pH 5.5 and pH 8.5 | Escherichia coli |