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ADP + (phosphate)20
ATP + (phosphate)19
-
-
-
-
r
ADP + (phosphate)45
ATP + (phosphate)44
ADP + (phosphate)65
ATP + (phosphate)64
ADP + (phosphate)n
ATP + (phosphate)n-1
ADP + (phosphate)n+1
ATP + (phosphate)n
ATP + (phosphate)45
ADP + (phosphate)46
ATP + (phosphate)65
ADP + (phosphate)66
ATP + (phosphate)n
ADP + (phosphate)n+1
ATP + (phosphate)n+1
ADP + (phosphate)n
-
-
-
r
ATP + ATP
ADP + (phosphate)n+1
-
polyP not required as primer
-
-
r
CDP + (phosphate)n
CTP + (phosphate)n-1
CDP + (phosphate)n+1
CTP + (phosphate)n
dADP + (phosphate)20
dATP + (phosphate)19
-
-
-
-
r
dADP + (phosphate)n+1
dATP + (phosphate)n
-
-
-
ir
dCDP + (phosphate)n+1
dCTP + (phosphate)n
-
-
-
ir
dGDP + (phosphate)n+1
dGTP + (phosphate)n
-
-
-
ir
GDP + (phosphate)20
GTP + (phosphate)19
-
isoform PPK1 affinity for the ADP as a substrate is about 70fold high as opposed to GDP
-
-
r
GDP + (phosphate)n
GTP + (phosphate)n-1
GDP + (phosphate)n+1
GTP + (phosphate)n
GDP + (phosphate)n+2
guanosine 5'-tetraphosphate + (phosphate)n
GTP + (phosphate)65
GDP + (phosphate)66
-
-
-
-
?
GTP + (phosphate)n
GDP + (phosphate)n+1
GTP + (phosphate)n+1
GDP + (phosphate)n
-
-
-
r
ITP + (phosphate)n
IDP + (phosphate)n+1
TDP + (phosphate)n+1
TTP + (phosphate)n
-
-
-
ir
UDP + (phosphate)20
UTP + (phosphate)19
-
-
-
-
r
UDP + (phosphate)n
UTP + (phosphate)n-1
UDP + (phosphate)n+1
UTP + (phosphate)n
additional information
?
-
ADP + (phosphate)45

ATP + (phosphate)44
-
similar catalytic activities with either (phosphate)65 or (phosphate)45. Isoform PPK2 catalyzes polyphosphate-dependent phosphorylation of ADP to ATP at a rate 838times higher than the rate of polyphosphate synthesis
-
-
r
ADP + (phosphate)45
ATP + (phosphate)44
-
similar catalytic activities with either (phosphate)65 or (phosphate)45. Isoform PPK2 catalyzes polyphosphate-dependent phosphorylation of ADP to ATP at a rate 838times higher than the rate of polyphosphate synthesis
-
-
r
ADP + (phosphate)45
ATP + (phosphate)44
-
-
-
-
r
ADP + (phosphate)65

ATP + (phosphate)64
-
similar catalytic activities with either (phosphate)65 or (phosphate)45. Isoform PPK2 catalyzes polyphosphate-dependent phosphorylation of ADP to ATP at a rate 838times higher than the rate of polyphosphate synthesis
-
-
r
ADP + (phosphate)65
ATP + (phosphate)64
-
similar catalytic activities with either (phosphate)65 or (phosphate)45. Isoform PPK2 catalyzes polyphosphate-dependent phosphorylation of ADP to ATP at a rate 838times higher than the rate of polyphosphate synthesis
-
-
r
ADP + (phosphate)65
ATP + (phosphate)64
-
-
-
-
r
ADP + (phosphate)n

ATP + (phosphate)n-1
-
-
-
-
?
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
-
r
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
r
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
-
?
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
-
?
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
-
r
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
?
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
?
ADP + (phosphate)n
ATP + (phosphate)n-1
not (phosphate)3, (phosphate)4 as preferred phosphate donor
-
-
r
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
-
r
ADP + (phosphate)n+1

ATP + (phosphate)n
-
-
-
r
ADP + (phosphate)n+1
ATP + (phosphate)n
-
-
-
r
ADP + (phosphate)n+1
ATP + (phosphate)n
-
polyphosphate kinase directly or indirectly regulates DNA polymerase activity or fidelity
-
-
-
ADP + (phosphate)n+1
ATP + (phosphate)n
-
substrates are polyphosphates or hexametaphosphate
-
r
ADP + (phosphate)n+1
ATP + (phosphate)n
-
no activity with GDP, diphosphate or tripolyphosphate
-
r
ADP + (phosphate)n+1
ATP + (phosphate)n
-
-
-
r
ADP + (phosphate)n+1
ATP + (phosphate)n
-
-
-
r
ADP + (phosphate)n+1
ATP + (phosphate)n
enzyme is involved in polyphosphate synthesis. The polyphosphate kinase gene is transcribed from a sigma(E) dependent promoter, which could be responsive to environmental stresses
-
-
?
ADP + (phosphate)n+1
ATP + (phosphate)n
-
GDP is preferred over ADP over nucleoside diphosphate acceptors
-
-
?
ADP + (phosphate)n+1
ATP + (phosphate)n
-
-
-
r
ADP + (phosphate)n+1
ATP + (phosphate)n
enzyme is involved in polyphosphate synthesis. The polyphosphate kinase gene is transcribed from a sigma(E) dependent promoter, which could be responsive to environmental stresses
-
-
?
ADP + (phosphate)n+1
ATP + (phosphate)n
-
GDP is preferred over ADP over nucleoside diphosphate acceptors
-
-
?
ADP + (phosphate)n+1
ATP + (phosphate)n
-
no activity with AMP
-
r
ADP + (phosphate)n+1
ATP + (phosphate)n
-
the enzyme protects Salmonella enterica from weak organic acid stress. Polyphosphate may acts as a chemical chaperone that helps refold homoserine transsuccinylase and/or may stimulate proteolysis of toxic denatured protein. The instability of homoserine transsuccinylase may provide a metabolic fuse that blocks growth under conditions that denature proteins. The sensitivity of this fuse is modulated by polyphosphate
-
-
?
ATP + (phosphate)45

ADP + (phosphate)46
-
-
-
-
r
ATP + (phosphate)45
ADP + (phosphate)46
-
-
-
-
r
ATP + (phosphate)65

ADP + (phosphate)66
-
-
-
-
r
ATP + (phosphate)65
ADP + (phosphate)66
-
-
-
-
r
ATP + (phosphate)n

ADP + (phosphate)n+1
Achromobacter butyri
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
phosphate polymers act as templates for polyphosphate synthesis, e.g. tetrapolyphosphate, trimetaphosphate or tetrametaphosphate
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
no activity with CTP, GTP and TTP
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
polyphosphate utilization and accumulation contribute significantly to Campylobacter jejuni pathogenesis and affect its ability to adapt to specific stresses and stringencies
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
polyphosphate utilization and accumulation contribute significantly to Campylobacter jejuni pathogenesis and affect its ability to adapt to specific stresses and stringencies
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
Chlamydomonas sp.
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
phosphate polymers act as templates for polyphosphate synthesis, e.g. tetrapolyphosphate, trimetaphosphate or tetrametaphosphate
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
PPK2B forms polyphopshate with an average chain length of about 125
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
PPK2B forms polyphopshate with an average chain length of about 125 and prefers the polyphosphate synthesis reaction direction
polyphosphate product chain length determination by NMR spectroscopy
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
PPK2B forms polyphopshate with an average chain length of about 125
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
PPK2B forms polyphopshate with an average chain length of about 125 and prefers the polyphosphate synthesis reaction direction
polyphosphate product chain length determination by NMR spectroscopy
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
DdPPK1 products are heterogeneous in chain length, with broad-range chain lengths of 50-300 Pi residues, and shorter compared to those of Escherichia coli
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
PPK1 is the principal enzyme responsible for reversible synthesis of polyphosphate from the terminal phosphate of ATP
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
polyphosphate chains of lenths of 700-800 residues
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
autophosphorylation, i.e. the gamma-phosphate of ATP becomes covalently attached to the enzyme under condition of polyphosphate synthesis, 0.52-0.92 mol phosphate per mol enzyme
via phosphorylated enzyme intermediate
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
best substrates are polyphosphates with more than 132 residues
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
no activity with phosphates of 5 residues or below
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
no primer substrate required
via phosphorylated enzyme intermediate
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
phosphate polymers act as templates for polyphosphate synthesis, e.g. tetrapolyphosphate, trimetaphosphate or tetrametaphosphate
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
incorporates gamma-phosphate of ATP into long-chain polyphosphate molecules
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
polyphosphate kinase may be involved in nucleotide metabolism
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
involved in phosphate metabolism of bacteria
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
phosphate polymers act as templates for polyphosphate synthesis, e.g. tetrapolyphosphate, trimetaphosphate or tetrametaphosphate
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
essential role of the enzyme during the initial steps of colonisation of the mouse gastric mucosa. The enzyme may act on the virulence of Helicobacter pylori partly through an energy dependent mechanism
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
phosphate polymers act as templates for polyphosphate synthesis, e.g. tetrapolyphosphate, trimetaphosphate or tetrametaphosphate
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
autophosphorylation, i.e. the gamma-phosphate of ATP becomes covalently attached to the enzyme under condition of polyphosphate synthesis, 0.52-0.92 mol phosphate per mol enzyme
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
autophosphorylation, i.e. the gamma-phosphate of ATP becomes covalently attached to the enzyme under condition of polyphosphate synthesis, 0.52-0.92 mol phosphate per mol enzyme
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
autophosphorylation, i.e. the gamma-phosphate of ATP becomes covalently attached to the enzyme under condition of polyphosphate synthesis, 0.52-0.92 mol phosphate per mol enzyme
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
strictly processive mechanism of polyphosphate elongation, phosphate or short-chains of polyphosphates serve as primers, majority of the synthesized polyphosphates is 750 residues long
polyphosphate as primer: product with 750 residues, phosphate as primer: product with 300-2000 residues, i.e. major form with chain length of 2000
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
Q9S646
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
polyphosphate kinase 1 and 2
polyphosphate with 200-800 residues, poyphosphate with 500-800 residues
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
phosphate polymers act as templates for polyphosphate synthesis, e.g. tetrapolyphosphate, trimetaphosphate or tetrametaphosphate
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
essential for biofilm development, quorum sensing, and virulence of Pseudomonas aeruginosa
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
PPK1 is essential for a successful ocular infection by the organism
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
very slow reverse reaction
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
no activity with AMP
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
enzyme synthesizes polyphosphates of 300000-400000 Da
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
ir
ATP + (phosphate)n
ADP + (phosphate)n+1
-
phosphate polymers act as templates for polyphosphate synthesis, e.g. tetrapolyphosphate, trimetaphosphate or tetrametaphosphate
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
incorporates gamma-phosphate of ATP into long-chain polyphosphate molecules
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
glycogen-bound polyphosphate kinase
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
the enzyme does not require the presence of histones for its activity
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
Q8KT90
-
-
-
r
CDP + (phosphate)n

CTP + (phosphate)n-1
-
-
-
?
CDP + (phosphate)n
CTP + (phosphate)n-1
-
-
-
?
CDP + (phosphate)n+1

CTP + (phosphate)n
-
-
-
ir
CDP + (phosphate)n+1
CTP + (phosphate)n
-
-
-
ir
CDP + (phosphate)n+1
CTP + (phosphate)n
-
the efficiency of polyphosphate utilization by PKK3 is 100%
-
-
?
GDP + (phosphate)n

GTP + (phosphate)n-1
-
-
-
r
GDP + (phosphate)n
GTP + (phosphate)n-1
-
-
-
-
?
GDP + (phosphate)n
GTP + (phosphate)n-1
-
-
-
-
?
GDP + (phosphate)n
GTP + (phosphate)n-1
-
-
-
?
GDP + (phosphate)n
GTP + (phosphate)n-1
-
-
-
?
GDP + (phosphate)n+1

GTP + (phosphate)n
-
-
-
-
GDP + (phosphate)n+1
GTP + (phosphate)n
-
efficiency of NDP substrates in descending order: ADP, dADP, dGDP, GDP, TDP, UDP, CDP, dCDP
-
ir
GDP + (phosphate)n+1
GTP + (phosphate)n
-
PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro
-
-
?
GDP + (phosphate)n+1
GTP + (phosphate)n
-
PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro
-
-
?
GDP + (phosphate)n+1
GTP + (phosphate)n
-
PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro
-
-
?
GDP + (phosphate)n+1
GTP + (phosphate)n
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro
-
-
?
GDP + (phosphate)n+1
GTP + (phosphate)n
-
10times higher activity than Escherichia coli polyphosphate kinase
-
ir
GDP + (phosphate)n+1
GTP + (phosphate)n
-
chains of 30-50 residues are optimal, chains of 15-700 residues can also serve. GDP is preferred over ADP over nucleoside diphosphate acceptors
-
-
?
GDP + (phosphate)n+1
GTP + (phosphate)n
-
chains of 30-50 residues are optimal, chains of 15-700 residues can also serve. GDP is preferred over ADP over nucleoside diphosphate acceptors
-
-
?
GDP + (phosphate)n+2

guanosine 5'-tetraphosphate + (phosphate)n
-
-
-
-
GDP + (phosphate)n+2
guanosine 5'-tetraphosphate + (phosphate)n
-
diphosphate group transfer
-
ir
GTP + (phosphate)n

GDP + (phosphate)n+1
-
-
-
-
r
GTP + (phosphate)n
GDP + (phosphate)n+1
-
lower activity compared to ATP
-
-
r
GTP + (phosphate)n
GDP + (phosphate)n+1
polyphosphate kinase 2
-
?
ITP + (phosphate)n

IDP + (phosphate)n+1
-
-
-
-
r
ITP + (phosphate)n
IDP + (phosphate)n+1
-
lower activity compared to ATP
-
-
r
UDP + (phosphate)n

UTP + (phosphate)n-1
-
-
-
?
UDP + (phosphate)n
UTP + (phosphate)n-1
-
-
-
?
UDP + (phosphate)n+1

UTP + (phosphate)n
-
-
-
ir
UDP + (phosphate)n+1
UTP + (phosphate)n
-
-
-
ir
additional information

?
-
-
accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview
-
-
-
additional information
?
-
-
PKK2B also shows NDP kinase activity
-
-
-
additional information
?
-
-
accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview
-
-
-
additional information
?
-
-
PKK2B also shows NDP kinase activity
-
-
-
additional information
?
-
-
the organism also has an actin-related poly P synthesizing complex, DdPPK2, that is KCl dependent
-
-
-
additional information
?
-
the organism also has an actin-related poly P synthesizing complex, DdPPK2, that is KCl dependent
-
-
-
additional information
?
-
-
the enzyme from Dictyostelium discoideum performs autophosphorylation and has an unique N-terminal extension of 370 amino acids, lacking homology with any known protein, that is necessary for its enzymatic activity, cellular localization, and physiological functions, overview
-
-
-
additional information
?
-
the enzyme from Dictyostelium discoideum performs autophosphorylation and has an unique N-terminal extension of 370 amino acids, lacking homology with any known protein, that is necessary for its enzymatic activity, cellular localization, and physiological functions, overview
-
-
-
additional information
?
-
the substrate specificity of FtPPK2 includes purine but not pyrimidine nucleotides. No activity with UDP and CDP as substrates. No formation of ADP or GDP from AMP or GMP
-
-
-
additional information
?
-
-
maximum PPK activity is observed for wild-type AG83 Leishmania donovani amastigotes when ADP is the phosphate group acceptor from polyphosphate, the activity is 29 times higher than with GDP as phosphate group acceptor from polyphosphate. When AMP is the phosphate group acceptor from polyphosphate, the activity of PPK is 97times less than with ADP, existence of both PPK1 and PPK2 activity is probable in Leishmania donovani amastigotes. ATP formation from AMP shows lowest activity
-
-
-
additional information
?
-
-
maximum PPK activity is observed for wild-type AG83 Leishmania donovani amastigotes when ADP is the phosphate group acceptor from polyphosphate, the activity is 29 times higher than with GDP as phosphate group acceptor from polyphosphate. When AMP is the phosphate group acceptor from polyphosphate, the activity of PPK is 97times less than with ADP, existence of both PPK1 and PPK2 activity is probable in Leishmania donovani amastigotes. ATP formation from AMP shows lowest activity
-
-
-
additional information
?
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview
-
-
-
additional information
?
-
-
PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase
-
-
-
additional information
?
-
-
the enzyme is unable to use triphosphate or pentaphosphate as phosphate donors
-
-
-
additional information
?
-
-
the enzyme is unable to use triphosphate or pentaphosphate as phosphate donors
-
-
-
additional information
?
-
-
PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase
-
-
-
additional information
?
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview
-
-
-
additional information
?
-
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview
-
-
-
additional information
?
-
-
PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase
-
-
-
additional information
?
-
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview
-
-
-
additional information
?
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase
-
-
-
additional information
?
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview
-
-
-
additional information
?
-
-
PA3455 protein is also active with (phosphate)3 as a phosphodonor, but its activity and substrate affinity are lower than those with (phosphate)12-13
-
-
-
additional information
?
-
gene ppk regulates the transcription accumulation of the stationary-phase sigma factor RpoS encoded by the rpoS gene, overview
-
-
-
additional information
?
-
all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity
-
-
-
additional information
?
-
all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity
-
-
-
additional information
?
-
all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity
-
-
-
additional information
?
-
-
all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity
-
-
-
additional information
?
-
all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity
-
-
-
additional information
?
-
all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity
-
-
-
additional information
?
-
all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity
-
-
-
additional information
?
-
-
the enzyme PPK from Streptomyces lividans also exhibits weak phospholipase D activity, EC 3.1.4.4, hydrolysing phosphatidylcholine, quantification of [3H]phosphatidic acid released from [3H]PC-labeled ELT3 cell membranes from phospholipase D-deficient cells, overview
-
-
-
additional information
?
-
-
production of choline from hydrolysis of 1,2-dioleoyl-sn-glycero-3-phosphocholine in the presence of the Amplex Red reagent by enzyme PPK
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ADP + (phosphate)n
ATP + (phosphate)n-1
ADP + (phosphate)n+1
ATP + (phosphate)n
ATP + (phosphate)n
ADP + (phosphate)n+1
CDP + (phosphate)n
CTP + (phosphate)n-1
CDP + (phosphate)n+1
CTP + (phosphate)n
dADP + (phosphate)n+1
dATP + (phosphate)n
-
-
-
ir
dCDP + (phosphate)n+1
dCTP + (phosphate)n
-
-
-
ir
dGDP + (phosphate)n+1
dGTP + (phosphate)n
-
-
-
ir
GDP + (phosphate)n
GTP + (phosphate)n-1
GDP + (phosphate)n+1
GTP + (phosphate)n
TDP + (phosphate)n+1
TTP + (phosphate)n
-
-
-
ir
UDP + (phosphate)n
UTP + (phosphate)n-1
UDP + (phosphate)n+1
UTP + (phosphate)n
additional information
?
-
ADP + (phosphate)n

ATP + (phosphate)n-1
-
-
-
r
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
-
?
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
-
?
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
?
ADP + (phosphate)n
ATP + (phosphate)n-1
-
-
-
?
ADP + (phosphate)n+1

ATP + (phosphate)n
-
polyphosphate kinase directly or indirectly regulates DNA polymerase activity or fidelity
-
-
-
ADP + (phosphate)n+1
ATP + (phosphate)n
enzyme is involved in polyphosphate synthesis. The polyphosphate kinase gene is transcribed from a sigma(E) dependent promoter, which could be responsive to environmental stresses
-
-
?
ADP + (phosphate)n+1
ATP + (phosphate)n
enzyme is involved in polyphosphate synthesis. The polyphosphate kinase gene is transcribed from a sigma(E) dependent promoter, which could be responsive to environmental stresses
-
-
?
ADP + (phosphate)n+1
ATP + (phosphate)n
-
the enzyme protects Salmonella enterica from weak organic acid stress. Polyphosphate may acts as a chemical chaperone that helps refold homoserine transsuccinylase and/or may stimulate proteolysis of toxic denatured protein. The instability of homoserine transsuccinylase may provide a metabolic fuse that blocks growth under conditions that denature proteins. The sensitivity of this fuse is modulated by polyphosphate
-
-
?
ATP + (phosphate)n

ADP + (phosphate)n+1
polyphosphate utilization and accumulation contribute significantly to Campylobacter jejuni pathogenesis and affect its ability to adapt to specific stresses and stringencies
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
polyphosphate utilization and accumulation contribute significantly to Campylobacter jejuni pathogenesis and affect its ability to adapt to specific stresses and stringencies
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
PPK2B forms polyphopshate with an average chain length of about 125
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
PPK2B forms polyphopshate with an average chain length of about 125
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
PPK1 is the principal enzyme responsible for reversible synthesis of polyphosphate from the terminal phosphate of ATP
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
polyphosphate kinase may be involved in nucleotide metabolism
-
-
ATP + (phosphate)n
ADP + (phosphate)n+1
-
involved in phosphate metabolism of bacteria
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
essential role of the enzyme during the initial steps of colonisation of the mouse gastric mucosa. The enzyme may act on the virulence of Helicobacter pylori partly through an energy dependent mechanism
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
Q9S646
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
PPK1 is essential for a successful ocular infection by the organism
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
-
?
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
-
-
-
r
ATP + (phosphate)n
ADP + (phosphate)n+1
Q8KT90
-
-
-
r
CDP + (phosphate)n

CTP + (phosphate)n-1
-
-
-
?
CDP + (phosphate)n
CTP + (phosphate)n-1
-
-
-
?
CDP + (phosphate)n+1

CTP + (phosphate)n
-
-
-
ir
CDP + (phosphate)n+1
CTP + (phosphate)n
-
-
-
ir
GDP + (phosphate)n

GTP + (phosphate)n-1
-
-
-
?
GDP + (phosphate)n
GTP + (phosphate)n-1
-
-
-
?
GDP + (phosphate)n+1

GTP + (phosphate)n
-
efficiency of NDP substrates in descending order: ADP, dADP, dGDP, GDP, TDP, UDP, CDP, dCDP
-
ir
GDP + (phosphate)n+1
GTP + (phosphate)n
-
10times higher activity than Escherichia coli polyphosphate kinase
-
ir
UDP + (phosphate)n

UTP + (phosphate)n-1
-
-
-
?
UDP + (phosphate)n
UTP + (phosphate)n-1
-
-
-
?
UDP + (phosphate)n+1

UTP + (phosphate)n
-
-
-
ir
UDP + (phosphate)n+1
UTP + (phosphate)n
-
-
-
ir
additional information

?
-
-
accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview
-
-
-
additional information
?
-
-
accumulation of high levels of cytosolic phosphorus in the form of polyphosphate involving classII polyphosphate kinase isozymes, granular poly P, i.e. volutin, can make up to 37% of the internal cell volume, overview
-
-
-
additional information
?
-
-
the organism also has an actin-related poly P synthesizing complex, DdPPK2, that is KCl dependent
-
-
-
additional information
?
-
the organism also has an actin-related poly P synthesizing complex, DdPPK2, that is KCl dependent
-
-
-
additional information
?
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview
-
-
-
additional information
?
-
-
PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase
-
-
-
additional information
?
-
-
PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase
-
-
-
additional information
?
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview
-
-
-
additional information
?
-
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview
-
-
-
additional information
?
-
-
PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase
-
-
-
additional information
?
-
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview
-
-
-
additional information
?
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase
-
-
-
additional information
?
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview
-
-
-
additional information
?
-
gene ppk regulates the transcription accumulation of the stationary-phase sigma factor RpoS encoded by the rpoS gene, overview
-
-
-
additional information
?
-
-
the enzyme PPK from Streptomyces lividans also exhibits weak phospholipase D activity, EC 3.1.4.4, hydrolysing phosphatidylcholine, quantification of [3H]phosphatidic acid released from [3H]PC-labeled ELT3 cell membranes from phospholipase D-deficient cells, overview
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ca2+
activates; activates; activates, best cation
Co2+
-
1.5-2.5fold lower activation than with Mg2+, inhibitory with Mg2+ as activator
Ni2+
activates; activates; activates
KCl

Dictyostelium discoideum also has an actin-related poly P synthesizing complex, DdPPK2, that is KCl dependent
Mg2+

-
maximal activity at 10 mM
Mg2+
-
maximal activation at 10 mM
Mg2+
-
optimal at 10 mM; optimal at 10 mM, activates
Mg2+
-
maximal activity at 12-13 mM; required for activity
Mg2+
-
required for activity
Mg2+
-
activation; maximal activity at 5-10 mM at 1 mM ATP
Mg2+
-
activation; maximal activity at 10 mM
Mg2+
-
maximal activity of polyphosphate synthesis, ATP and GTP synthesis, and guanosine 5'-tetraphosphate synthesis at 5.0, 2.0, 1.0 and 0.2 mM
Mg2+
required, best at 10 mM
Mg2+
optimal above 0.5 mM
Mg2+
-
optimum activity at 30 mM Mg2+
Mg2+
-
optimum Mg2+ concentration is 5 mM
Mg2+
-
necessary for activity
Mg2+
-
maximal activity at 2 mM; required for activity
Mg2+
; polyphosphate kinase 2, preferred in ATP synthesis over Mn2+
Mg2+
-
5fold higher activation than Mn2+, at optimal concentration of 10 mM
Mg2+
-
no activity in the absence of a divalent metal cation, Mg2+ is the most effective metal, whereas low activity is observed with Co2+ or Ni2+ and no activity is observed in the presence of Mn2+ or Ca2+
Mg2+
recombinant polyphosphate kinase, maximal activity at 10 mM
Mg2+
activates; activates; activates, best cation
Mg2+
-
active substrate: MgATP; inhibitory above 6 mM; required for activity
Mg2+
no activity in the absence of a divalent metal cation, Mg2+ is the most effective metal, whereas low activity is observed with Co2+ or Ni2+ and no activity is observed in the presence of Mn2+ or Ca2+
Mg2+
-
50% as effective as Mn2+; activation; stimulates
Mg2+
; optimal above 0.5 mM
Mn2+

-
can replace Mg2+ to some extent
Mn2+
-
optimal at 10 mM; optimal at 10 mM, activates highly
Mn2+
-
activation, can replace Mg2+
Mn2+
-
2 mM, 22% of activation with Mg2+; activation, can replace Mg2+
Mn2+
-
1.5-2.5fold lower activation than with Mg2+; activation, can replace Mg2+
Mn2+
required, best at 1 mM
Mn2+
-
optimum activity at 1 mM Mn2+
Mn2+
-
necessary for activity
Mn2+
-
activation, can replace Mg2+; slightly
Mn2+
polyphosphate kinase 2, 10 mM preferred in polyphosphate synthesis over Mg2+
Mn2+
-
Mg2+ shows 5fold higher activation than Mn2+, at optimal concentration of 10 mM
Mn2+
activates; activates; activates, best cation
Mn2+
-
activation, can replace Mg2+; active substrate: MnATP; maximal activity at 1.0-2.0 mM, inhibition above 2 mM
Mn2+
-
maximal activity at 2 mM; stimulates, preferred metal ion
Zn2+

-
activation, 0.4 mM, 10% as effective as Mg2+
Zn2+
activates; activates; activates
additional information

-
not activated by Ca2+
additional information
lower optimal concentration for Mn2+ ions than Mg2+ ions
additional information
-
not activated by Na+ and polyphosphate
additional information
-
Fe2+, Zn2+, Cu2+ cannot be used by the enzyme
additional information
-
not activated by Ca2+
additional information
enzyme SPO1727 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview; isozyme SPO0224 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview; isozyme SPO125 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview
additional information
enzyme SPO1727 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview; isozyme SPO0224 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview; isozyme SPO125 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview
additional information
enzyme SPO1727 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview; isozyme SPO0224 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview; isozyme SPO125 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview
additional information
-
enzyme SPO1727 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview; isozyme SPO0224 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview; isozyme SPO125 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview
additional information
-
not activated by NH4Cl
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ammonium hydrochloride
-
50% inhibition at 200 mM
Ca2+
-
1 mM, 10% inhibition
Cu2+
-
0.1 mM, 92% inhibition, 1 mM, complete inhibition
ellagic acid
Q9S646
ellagic acid derivatives from Terminalia chebula increase the susceptibility of Pseudomonas aeruginosa to stress by inhibiting polyphosphate kinase, oveview. Ellagic acid derivatives completely inhibit PPK1 activity at 0.5 mg/ml. Ellagic acid derivatives-treated Pseudomonas aeruginosa cells show marked reduction in polyphosphate granules in cytosol. Ellagic acid derivatives from Terminalia chebula inhibit PPK1 expression and its activity and increase the sensitivity of Pseudomonas aeruginosa to desiccation and oxidative stress while reducing tolerance to piperacillin
Guanidine HCl
-
5 mM, 50% inhibition of polyphosphate synthesis
histone
-
reverse reaction, strong, activates forward reaction in the presence of phosphate
hydrolyzed glycogen
-
complete inhibition
-
MnATP2-
-
at high concentrations, above 0.6 mM, activating below
NH4Cl
-
200 mM, approx. 50% inhibition of glycogen-bound polyphosphate kinase
phalloidin
a heptapeptide toxin from the mushroom Amanita phalloides, which binds tightly and specifically to polymerized actin, inhibits DdPPK2 and DdPPK1, the latter by 80% at 10 nM, the molar ratio of phalloidin to DdPPK1 of 10:15 results in complete inhibition of DdPPK1 activity
Sodium fluoride
-
slight inhibition
Zn2+
-
0.1 mM, 67% inhibition, 1 mM, complete inhibition
(NH4)2SO4

-
40 mM, almost complete inhibition
(NH4)2SO4
-
activation up to 100 mM; inhibition at higher concentrations; strong inhibition
(NH4)2SO4
-
strong inhibition
ADP

-
0.025 mM, 0.05 mM, 0.1 mM and 0.25 mM, 56%, 68%, 77% and 100% inhibition, respectively of polyphosphate synthesis in crude extracts
ADP
-
0.08 mM, complete inhibition
ADP
-
0.2 mM, complete inhibition
ADP
-
50%, 70% and 93% inhibition at 0.08 mM, 0.15 mM and 0.2 mM ADP, respectively
ADP
-
competitive inhibition
ADP
-
substrate inhibition
ADP
Q9S646
product inhibition
ADP
-
0.25 mM, approx. 50% inhibition, 1 mM, complete inhibition
ADP
-
4 mM, product inhibition
ADP
-
2 mM, 50% inhibition of glycogen-bound polyphosphate kinase; product inhibition, about 50% inhibition at 2 mM
AMP

-
-
AMP
-
at high concentrations
AMP
-
15% and 70% inhibition of the forward reaction at AMP/ADP ratios of 1 and 10, respectiverly
ATP

-
substrate inhibition, at high concentrations
ATP
-
free form, above 0.008 mM
ATP
-
20% substrate inhibition at 2.4 mM
Co2+

-
1 mM, 40% inhibition
Co2+
complete inhibition; complete inhibition; complete inhibition
diphosphate

-
weak inhibition
diphosphate
-
1 mM, complete inhibition
diphosphate
-
10 mM, 66% inhibition of polyphosphate synthesis, 75% inhibition of GTP synthesis
diphosphate
-
1 mM, complete inhibition
EDTA

complete inhibition
EDTA
-
10 mM, complete inhibition
EDTA
low inhibition; low inhibition
F-

-
5 mM, complete inhibition
F-
-
20 mM, complete inhibition
F-
-
5 mM, 75% inhibition
F-
-
10 mM, complete inhibition
F-
-
20 mM, complete inhibition
F-
-
2 mM, 25% inhibition of glycogen-bound polyphosphate kinase
GMP

-
-
GMP
-
competitive inhibition of polyphosphate 750 and GDP in guanosine 5'-tetraphosphate synthesis
KCl

inhibits PPK1 80% at 500 mM, but not PPK2
KCl
-
50 mM, 50% inhibition
Mg2+

-
8 mM, complete inhibition
Mg2+
-
free form, above 6 mM, activating below
Mn2+

-
weak inhibition at 10 mM Mg2+
Mn2+
-
free form, above 2 mM, activating below
phosphate

-
above 20 mM; activates in the presence of histone
phosphate
-
5.5 mM and 11 mM, 33% and 49% inhibition of forward reaction
phosphate
-
2 mM, 50% inhibition of glycogen-bound polyphosphate kinase; strong inhbition
Polyphosphate

-
65 residues, competitive inhibition of polyphosphate 750 and GDP in guanosine 5'-tetraphosphate synthesis
Polyphosphate
-
substrate inhibition at 2 mM
additional information

-
no inhibition by UMP and CMP
-
additional information
-
not inhibited by NaCl
-
additional information
-
not inhibited by 2,4-dinitrophenol or potassium arsenate
-
additional information
-
not inhibited by 3',5'-adenosine monophosphate
-
additional information
MIC values of wild-type and mutant strain ExPEC for diverse antibiotics, i.e. beta-lactams cefotaxime, ceftazidime, ampicillin, cefazolin, tazobactam, and ticarcillin, glycopeptide vancomycin, aminoglycosides gentamicin, gentamicin sulfate, amikacin macrolide, erythromycin, quinolones norfloxacin and levofloxacin, sulfonamide trimethoprim, sulfadiazine nitrofurans macrodantin, and lipopeptde polymyxin B, overview. Biofilm-grown DELTAppk and wild-type cells are similarly tolerant and more tolerant than planktonic cells
-
additional information
-
not inhibited by bafilomycin A1 or a protonophore
-
additional information
-
not inhibited by KCl; not inhibited by NaCl; not inhibited by phosphate; not inhibited by polyphosphate
-
additional information
-
a G9-quadruplex DNA aptamer inhibits isoform PPK2 with an IC50 of 40 nM and exhibits noncompetitive inhibition kinetics
-
additional information
-
enzymatic hydrolysis of glycogen
-
additional information
-
a G9-quadruplex DNA aptamer inhibits isoform PPK2 with an IC50 of 105 nM and exhibits noncompetitive inhibition kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.005 - 0.006
(phosphate)45
-
0.0054 - 0.0063
(phosphate)65
-
1
ITP
-
; pH 7.2, 30°C, recombinant wild-type enzyme
0.53
MnATP2-
-
pH 7.0, 30°C
1.67
phosphate
-
pH 7.0, 30°C
3.6
UDP
pH 8.0, 30°C, recombinant isozyme PPK2-3
additional information
additional information
-
0.005
(phosphate)45

-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
0.006
(phosphate)45
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
0.0054
(phosphate)65

-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
0.0063
(phosphate)65
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
0.072
ADP

-
pH 5.5, 25°C
0.1
ADP
-
in 50 mM Tris-HCl pH-7.5, 10 mM NH4SO4,10 mM KPO4, pH 7.0, 4 mM MgCl2, 50 mM NaCl, at 37°C
0.16
ADP
-
isoform PPK1, pH and temperature not specified in the publication
0.176
ADP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
0.18
ADP
-
pH 6.5-8.5, 25°C
0.18
ADP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
0.2
ADP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
0.38
ADP
-
50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
2
ADP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
2.5
ADP
pH 8.0, 30°C, recombinant isozyme PPK2-2
6.8
ADP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
8
ADP
-
isoform PPK2, pH and temperature not specified in the publication
0.075
ATP

-
pH 7.0, 75°C; pH 7.0, 75°C, glycogen-complexed enzyme
0.17
ATP
-
; pH 7.2, 30°C, recombinant wild-type enzyme
0.33
ATP
-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
0.8
ATP
-
in 50 mM Tris-HCl pH-7.5, 10 mM NH4SO4,10 mM KPO4, pH 7.0, 4 mM MgCl2, 50 mM NaCl, at 37°C
0.83
ATP
-
pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, detrmined with Robinson and Wood method
1.18
ATP
-
pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, kinetic analysis
1.2
ATP
pH 7.2, room temperature, recombinant polyphosphate kinase
1.35
ATP
-
isoform PPK1, pH and temperature not specified in the publication
1.38
ATP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
1.47
ATP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
1.5 - 2
ATP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
5.2
ATP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
13
ATP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
0.16
GDP

-
pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis
1.2
GDP
pH 8.0, 30°C, recombinant isozyme PPK2-1
11.3
GDP
-
isoform PPK1, pH and temperature not specified in the publication
0.14
GTP

-
; pH 7.2, 30°C, recombinant wild-type enzyme
0.66
GTP
-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
0.035
Polyphosphate

-
pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis
15
Polyphosphate
-
pH 7.2, 30°C, recombinant wild-type enzyme
additional information
additional information

-
exponential kinetics
-
additional information
additional information
Michalis-Menten steady state kinetic analysis of FtPPK2 substrate specificity, overview
-
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0.02 - 50
(phosphate)45
-
0.02 - 53
(phosphate)65
-
43
ITP
-
pH 7.2, 30°C, recombinant wild-type enzyme
0.02
(phosphate)45

-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
50
(phosphate)45
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
0.02
(phosphate)65

-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
53
(phosphate)65
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
3.17
ADP

pH 8.0, 37°C
5.22
ADP
pH 8.0, 30°C, recombinant isozyme PPK2-2
41.9
ADP
-
50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
210
ADP
-
isoform PPK2, pH and temperature not specified in the publication
332
ADP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
622
ADP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
943
ADP
-
isoform PPK1, pH and temperature not specified in the publication
950
ADP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
971
ADP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
994
ADP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
0.05
ATP

-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
44.6
ATP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
45
ATP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
45.6
ATP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
46.9
ATP
-
isoform PPK1, pH and temperature not specified in the publication
55
ATP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
55.4
ATP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
74
ATP
-
pH 7.2, 30°C, recombinant wild-type enzyme
0.0883
GDP

-
pH 7.2, 37°C
0.482
GDP
-
pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis
4.18
GDP
pH 8.0, 30°C, recombinant isozyme PPK2-1
645
GDP
-
isoform PPK1, pH and temperature not specified in the publication
0.08
GTP

-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
34
GTP
-
pH 7.2, 30°C, recombinant wild-type enzyme
1.04
Polyphosphate

-
pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis
77
Polyphosphate
-
pH 7.2, 30°C, recombinant wild-type enzyme
0.117
UDP

-
pH 7.2, 37°C
4.9
UDP
pH 8.0, 30°C, recombinant isozyme PPK2-3
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3.704 - 8400
(phosphate)65
-
1.37
UDP
pH 8.0, 30°C, recombinant isozyme PPK2-3
4
(phosphate)45

-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
8300
(phosphate)45
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
3.704
(phosphate)65

-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
8400
(phosphate)65
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
2.08
ADP

pH 8.0, 30°C, recombinant isozyme PPK2-2
25.5
ADP
-
isoform PPK2, pH and temperature not specified in the publication
49
ADP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
110
ADP
-
50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
998
ADP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
3529
ADP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
4690
ADP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
5222
ADP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
5743
ADP
-
isoform PPK1, pH and temperature not specified in the publication
0.15
ATP

-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
4.2
ATP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
10.5
ATP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
29
ATP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
31
ATP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
32.6
ATP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
34.7
ATP
-
isoform PPK1, pH and temperature not specified in the publication
3.48
GDP

pH 8.0, 30°C, recombinant isozyme PPK2-1
57
GDP
-
isoform PPK1, pH and temperature not specified in the publication
0.121
GTP

-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
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