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Literature summary for 2.7.11.12 extracted from

  • Chan, M.H.; Aminzai, S.; Hu, T.; Taran, A.; Li, S.; Kim, C.; Pilz, R.B.; Casteel, D.E.
    A substitution in cGMP-dependent protein kinase 1 associated with aortic disease induces an active conformation in the absence of cGMP (2020), J. Biol. Chem., 295, 10394-10405 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
R177A the mutation in isoform PKG1alpha increases the basal activity of the enzyme Homo sapiens
R177M the mutation in isoform PKG1alpha increases the basal activity of the enzyme Homo sapiens
R177Q the mutation in isoform PKG1alpha increases PKG1alpha autophosphorylation in vitro and causes a high basal kinase activity (90-95%) in the absence of cGMP Homo sapiens
R192Q the mutation in isoform in PKG1beta increases PKG1beta autophosphorylation in vitro and causes a high basal kinase activity in the absence of cGMP Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
8-(4-chlorophenylthio)-beta-phenyl-1,N2-ethenoguanosine-3'5'-cyclic monophophorothioate, Rp isomer
-
 Homo sapiens
8-(4-chlorophenylthio)-guanosine-3'5'-cyclic monophophosphate
-
 Homo sapiens
additional information the Rp-isomer of 8-(4-chlorophenylthio)guanosine-3'5'-cyclic monophophorothioate does not inhibit the activity of the enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 30 mM used in assay conditions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Kemptide
-
 Homo sapiens ADP + phosphorylated Kemptide
-
 ?
ATP + VASP protein
-
 Homo sapiens ADP + phosphorylated VASP protein
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 75000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
cGMP-dependent protein kinase 1
-
 Homo sapiens
PKG1alpha isoform Homo sapiens
PKG1beta isoform Homo sapiens