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Literature summary for 2.7.11.12 extracted from

  • Isshiki, K.; Matsuda, S.; Tsuji, A.; Yuasa, K.
    cGMP-dependent protein kinase I promotes cell apoptosis through hyperactivation of death-associated protein kinase 2 (2012), Biochem. Biophys. Res. Commun., 422, 280-284.
    View publication on PubMed
Search for more literature for 2.7.11.12

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + DAPK2 protein Homo sapiens i.e death-associated protein kinase 2, phosphorylation at Ser299, Ser367 and Ser368 type I cGMP-dependent protein kinase ADP + DAPK2 phosphoprotein
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + DAPK2 protein i.e death-associated protein kinase 2, phosphorylation at Ser299, Ser367 and Ser368 type I cGMP-dependent protein kinase Homo sapiens ADP + DAPK2 phosphoprotein
-
 ?
ATP + DAPK2 protein i.e death-associated protein kinase 2, phosphorylation at Ser299, Ser367 and Ser368 type I cGMP-dependent protein kinase, recombinant wild-type and mutant FLAG-tagged DAPK2 expressed in COS-7 cells, substrate detection from microarray substrate screening, overview Homo sapiens ADP + DAPK2 phosphoprotein
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 ?

Synonyms

Synonyms Comment Organism
cGK-I
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 Homo sapiens
cGMP-dependent protein kinase I
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 Homo sapiens
cGMP-dependent protein kinase-I
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 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism importance of the cGK-I/DAPK2 signaling pathway in regulating apoptosis, overview Homo sapiens
additional information consensus motif for cGK is RR/KXS/T Homo sapiens
physiological function cGMP-dependent protein kinase-I (cGK-I) induces apoptosis in various cancer cell lines, and death-associated protein kinase 2, DAPK2, is one of the targets of cGK-I in apoptosis induction. Phosphorylation of DAPK2 at Ser299 by cGKI enhances DAPK2 kinase activity, but overexpression of a phospho-mimic DAPK2 mutant, DAPK2 S299D, as well as mutant S299D/S318E, strongly induce apoptosis in human breast cancer MCF-7 cells compared with wild-type DAPK2, while a S367D/S368D mutant does not Homo sapiens