Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1D-myo-inositol 1,3,4,5-tetrakisphosphate | recombinant, catalytically active fragment of isoform C, allosteric product activation in the absence of Ca2+/calmodulin, which per se activates enzyme and abolishes the allosteric effect | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
cloning of a full-length cDNA encoding IP3K isoform C from tongue epithelium, cloning of an enzymatically active and Ca2+/calmodulin-regulated fragment of isoform C and expression in Escherichia coli BL21(DE3)pRIL | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1D-myo-inositol 1,3,4,5-tetrakisphosphate | marked product inhibition, isoforms A and B | Rattus norvegicus | |
1D-myo-inositol 1,4,5-trisphosphate | recombinant, catalytically active fragment of isoform C, substrate inhibition by high concentrations | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.5, 30°C, recombinant, catalytically active fragment of isoform C | Rattus norvegicus | |
0.0015 | - |
1D-myo-inositol 2,4,5-trisphosphate | pH 7.5, 30°C, in absence of Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C | Rattus norvegicus | |
0.005 | - |
1D-myo-inositol 2,4,5-trisphosphate | pH 7.5, 30°C, in presence of 100 nM Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C | Rattus norvegicus | |
0.033 | - |
ATP | pH 7.5, 30°C, in absence of Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C | Rattus norvegicus | |
0.052 | - |
ATP | pH 7.5, 30°C, in presence of 100 nM Ca2+/calmodulin, recombinant, catalytically active fragment of isoform C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | isoform C undergoes active nucleocytoplasmic shuttling | Rattus norvegicus | 5737 | - |
nucleus | isoform C is actively transported into and out of the nucleus, a leucine-rich nuclear export signal and a nuclear import activity are localized in the N-terminal domain | Rattus norvegicus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | isoform C is strongly activated by Ca2+/calmodulin in a substrate concentration-dependent manner | Rattus norvegicus | |
Ca2+ | activation by Ca2+/calmodulin | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74500 | - |
x * 74500, calculated from the amino acid sequence | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | inositol 1,4,5-trisphosphate is a second messenger | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | IP3K isoform C: nucleocytoplasmic shuttling, particular role in nuclear inositol trisphosphate phosphorylation and cellular growth, enzyme seems to function during the intervalls between signaling events, keeps the resting Ins(1,4,5)P3 very low and provides a basal Ins(1,3,4,5)P4 production in the absence of stimulatory signals from the exterior | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | inositol phosphate metabolism | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
isoform C | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | isoform C | Rattus norvegicus | - |
heart | isoform C | Rattus norvegicus | - |
kidney | isoform C, low expression | Rattus norvegicus | - |
liver | isoform C, low expression | Rattus norvegicus | - |
lung | isoform C, low expression | Rattus norvegicus | - |
testis | isoform C | Rattus norvegicus | - |
tongue | strongest expression of isoform C in tongue epithelium | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | domain structure of IP3K-C | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | specific for phosphorylation of the 3-position | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol 1,4,5-trisphosphate is a second messenger | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | IP3K isoform C: nucleocytoplasmic shuttling, particular role in nuclear inositol trisphosphate phosphorylation and cellular growth, enzyme seems to function during the intervalls between signaling events, keeps the resting Ins(1,4,5)P3 very low and provides a basal Ins(1,3,4,5)P4 production in the absence of stimulatory signals from the exterior | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol phosphate metabolism | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 2,4,5-trisphosphate | recombinant, catalytically active fragment of isoform C, in the presence of Ins(1,3,4,5)P4, authentic side activity of isoform C | Rattus norvegicus | ADP + 1D-myo-inositol 2,4,5,6-tetrakisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 74500, calculated from the amino acid sequence | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus | |
Calmodulin | activation by Ca2+/calmodulin | Rattus norvegicus | |
Calmodulin | isoform C is strongly activated by Ca2+/calmodulin in a substrate concentration-dependent manner | Rattus norvegicus |