Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Nicotiana tabacum |
Crystallization (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(Z)-3-(2-phosphonoethen-1-yl)pyridine-2-carboxylic acid | - |
Nicotiana tabacum | |
3-(phosphonomethyl)pyridine-2-carboxylic acid | - |
Nicotiana tabacum | |
4-(phosphonomethyl)pyridine-2-carboxylic acid | - |
Nicotiana tabacum | |
DL-(E)-2-amino-5-phosphono-3-pentenoic acid | - |
Nicotiana tabacum | |
DL-propargylglycine | irreversible | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
L-cysteine | - |
Nicotiana tabacum | |
0.55 | - |
Sodium sulfide | - |
Nicotiana tabacum | |
0.89 | - |
L-cysteine methyl ester | - |
Nicotiana tabacum | |
2.6 | - |
2-mercaptoethanol | - |
Nicotiana tabacum | |
7.1 | - |
O-phospho-L-homoserine | - |
Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-succinyl-L-homoserine + L-cysteine | Nicotiana tabacum | the enzyme catalyzes the first reaction specific for methionine biosynthesis | L-cystathionine + succinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Nicotiana tabacum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate | ping-pong mechanism | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-homoserine + L-Cys methyl ester | 26% of the activity with L-Cys | Nicotiana tabacum | S-(L-2-amino-2-methoxycarbonyl)-L-homocysteine + acetate | - |
? | |
O-phospho-L-homoserine + L-cysteine | - |
Nicotiana tabacum | L-cystathionine + phosphate | - |
? | |
O-succinyl-L-homoserine + 2-mercaptoethanol | 32% of the activity with L-cysteine | Nicotiana tabacum | S-ethyl-L-homocysteine + succinate | - |
? | |
O-succinyl-L-homoserine + D-cysteine | 20% of the activity with L-cysteine | Nicotiana tabacum | ? | - |
? | |
O-succinyl-L-homoserine + L-cysteine | the enzyme catalyzes the first reaction specific for methionine biosynthesis | Nicotiana tabacum | L-cystathionine + succinate | - |
? | |
O-succinyl-L-homoserine + sodium sulfide | 62% of the activity with L-cysteine | Nicotiana tabacum | L-homocysteine + sodium succinate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | enzyme is dependent on | Nicotiana tabacum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
DL-propargylglycine | - |
Nicotiana tabacum | |
0.027 | - |
DL-(E)-2-amino-5-phosphono-3-pentenoic acid | - |
Nicotiana tabacum | |
0.2 | - |
3-(phosphonomethyl)pyridine-2-carboxylic acid | - |
Nicotiana tabacum | |
0.3 | - |
4-(phosphonomethyl)pyridine-2-carboxylic acid | - |
Nicotiana tabacum | |
0.45 | - |
(Z)-3-(2-phosphonoethen-1-yl)pyridine-2-carboxylic acid | - |
Nicotiana tabacum |