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Literature summary for 2.5.1.18 extracted from
- Molecular cloning, biochemical characterization, and expression analysis of two glutathione S-transferase paralogs from the big-belly seahorse (Hippocampus abdominalis) (2017), Comp. Biochem. Physiol. B, 214, 1-11 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2523 cells | Hippocampus abdominalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cibacron blue | 91% inhibition at 0.1 mM; 94% inhibition at 0.1 mM | Hippocampus abdominalis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTrho, at pH 6.5 and 25°C | Hippocampus abdominalis | |
| 0.63 | - |
1-chloro-2,4-dinitrobenzene | isoform GSTmu, at pH 6.5 and 25°C | Hippocampus abdominalis | |
| 0.78 | - |
glutathione | isoform GSTrho, at pH 6.5 and 25°C | Hippocampus abdominalis | |
| 4.21 | - |
glutathione | isoform GSTmu, at pH 6.5 and 25°C | Hippocampus abdominalis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hippocampus abdominalis | A0A290DQ41 | big-bellied seahorse | - |
| Hippocampus abdominalis | A0A290DQJ2 | big-bellied seahorse | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose resin column chromatography | Hippocampus abdominalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | - |
Hippocampus abdominalis | - |
| blood | lowest expression of isoform GSTmu | Hippocampus abdominalis | - |
| brain | - |
Hippocampus abdominalis | - |
| brood pouch | - |
Hippocampus abdominalis | - |
| gill | - |
Hippocampus abdominalis | - |
| gill | highest expression of isoform GSTrho | Hippocampus abdominalis | - |
| heart | - |
Hippocampus abdominalis | - |
| intestine | - |
Hippocampus abdominalis | - |
| kidney | - |
Hippocampus abdominalis | - |
| liver | - |
Hippocampus abdominalis | - |
| muscle | - |
Hippocampus abdominalis | - |
| ovary | - |
Hippocampus abdominalis | - |
| ovary | highest expression of isoform GSTmu | Hippocampus abdominalis | - |
| skin | - |
Hippocampus abdominalis | - |
| spleen | - |
Hippocampus abdominalis | - |
| spleen | lowest expression of isoform GSTrho | Hippocampus abdominalis | - |
| stomach | - |
Hippocampus abdominalis | - |
| testis | - |
Hippocampus abdominalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
isoform GSTmu, with ethacrynic acid as substrate, at pH 6.5 and 25°C | Hippocampus abdominalis |
| 4.41 | - |
isoform GSTmu, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C | Hippocampus abdominalis |
| 5.35 | - |
isoform GSTmu, with 4-nitrobenzyl chloride as substrate, at pH 6.5 and 25°C | Hippocampus abdominalis |
| 6.79 | - |
isoform GSTrho, with 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.5 and 25°C | Hippocampus abdominalis |
| 21.03 | - |
isoform GSTmu, with 4-nitrophenethyl bromide as substrate, at pH 6.5 and 25°C | Hippocampus abdominalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Hippocampus abdominalis | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
| glutathione + 4-nitrobenzyl chloride | - |
Hippocampus abdominalis | chloride + 4-nitrobenzyl-glutathione | - |
? | |
| glutathione + 4-nitrophenethyl bromide | - |
Hippocampus abdominalis | bromide + 4-nitrophenylethyl-glutathione | - |
? | |
| glutathione + ethacrynic acid | - |
Hippocampus abdominalis | ? | - |
? | |
| additional information | isoform GSTmu shows no activity with 1,2-dichloro-4-nitrobenzene | Hippocampus abdominalis | ? | - |
- |
|
| additional information | isoform GSTrho shows no activity with 1,2-dichloro-4-nitrobenzene, 4-nitrobenzyl chloride, 4-nitrophenethyl bromide and ethacrynic acid | Hippocampus abdominalis | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25740, isoform GSTmu, calculated from amino acid sequence | Hippocampus abdominalis |
| ? | x * 26060, isoform GSTrho, calculated from amino acid sequence | Hippocampus abdominalis |
| ? | x * 68240, recombinant isoform GSTmu bound to maltose-binding protein, SDS-PAGE | Hippocampus abdominalis |
| ? | x * 68590, recombinant isoform GSTrho bound to maltose-binding protein, SDS-PAGE | Hippocampus abdominalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Hippocampus abdominalis |
| GSTmu | isoform | Hippocampus abdominalis |
| GSTrho | isoform | Hippocampus abdominalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Hippocampus abdominalis |
| 40 | - |
- |
Hippocampus abdominalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Hippocampus abdominalis |
| 10 | - |
- |
Hippocampus abdominalis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hippocampus abdominalis | isoform GSTmu, calculated from amino acid sequence | - |
8.2 |
| Hippocampus abdominalis | isoform GSTrho, calculated from amino acid sequence | - |
9.1 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00045 | - |
at pH 6.5 and 25°C | Hippocampus abdominalis | Cibacron blue | |
| 0.0072 | - |
at pH 6.5 and 25°C | Hippocampus abdominalis | Cibacron blue | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Hippocampus abdominalis | mRNA expression of isoform GSTmu is significantly elevated upon lipopolysaccharide, Poly (I:C), and Edwardsiella tarda challenges in liver and in blood cells as well as with Streptococcus iniae challenge in blood cells | up |
| Hippocampus abdominalis | mRNA expression of isoform GSTrho is significantly elevated upon lipopolysaccharide, Poly (I:C), and Edwardsiella tarda challenges in liver and in blood cells as well as with Streptococcus iniae challenge in blood cells | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme isoform GSTrho is effective in detoxifying xenobiotic toxic agents | Hippocampus abdominalis |
| physiological function | the enzyme isoforms GSTrho and GSTmu are effective in detoxifying xenobiotic toxic agents | Hippocampus abdominalis |
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