Cloned (Comment) | Organism |
---|---|
transient expression of His6-tagged wild-type hGSTZ1-1 and mutant seleno-hGSTZ1-1 in HEK-293T cells, expression of GSTZ1-1 in Escherichia coli strain M15 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | site-directed mutagenesis and the inclusion of a selenocysteine insertion sequence to engineer the expression in eukaryotic cells of human glutathione transferase zeta1-1 with selenocysteine in the active site. This modification converts hGSTZ1-1 into an active glutathione peroxidase, EC 1.11.1.9, while the wild-type GSTZ1-1 shows no glutathione peroxidase at all, Michaelis-Menten kinetics and ping-pong mechanism of mutant Se-GSTZ1-1 with H2O2 and GSH, overview. Selenocysteine at the active site plays an important role in the determination of catalytic specificity and performance, the ping-pong catalytic mechanism of Se-hGSTZ1-1 is similar to that of the natural glutathione peroxidase | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24300 | - |
x * 24300, recombinant His6-tagged wild-type GSTZ1-1, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type hGSTZ1-1 and mutant seleno-hGSTZ1-1 from HEK-293T cells by nickel affinity chromatography | Homo sapiens |
Subunits | Comment | Organism |
---|---|---|
? | x * 24300, recombinant His6-tagged wild-type GSTZ1-1, SDS-PAGE | Homo sapiens |
More | wild-type hGSTZ1-1 and mutant seleno-hGSTZ1-1 three-dimensional structure modeling, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
selenium-containing glutathione transferase zeta1-1 | - |
Homo sapiens |
seleno-hGSTZ1-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |