Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
DELTA247-306 | deletion of the parasite-specific insertion: Km values for (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate and p-aminobenzoate are increased compared to mutant DELTA257-306 but still decreased compared to wild type, catalytic efficacy (kcat) is comparable to wild type | Plasmodium falciparum |
DELTA257-306 | deletion of the parasite-specific insertion: Km values for (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate and p-aminobenzoate are significantly decreased compared to wild type, catalytic efficacy (kcat) is at least 2fold decreased compared to wild type | Plasmodium falciparum |
DELTA74-80 | deletion of the parasite-specific insertion: Km values for (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate and p-aminobenzoate are 2 to 3 fold higher compared to wild type, catalytic efficacy (kcat) is at least 2fold decreased compared to wild type | Plasmodium falciparum |
DELTA74-86 | no DHPS activity is detected | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
p-Aminobenzoate | mutant DELTA257-306 | Plasmodium falciparum | |
0.0036 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA257-306 | Plasmodium falciparum | |
0.0039 | - |
p-Aminobenzoate | wild type | Plasmodium falciparum | |
0.0063 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA247-306 | Plasmodium falciparum | |
0.00708 | - |
p-Aminobenzoate | mutant DELTA74-80 | Plasmodium falciparum | |
0.008 | - |
p-Aminobenzoate | mutant DELTA247-306 | Plasmodium falciparum | |
0.0148 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | wild type | Plasmodium falciparum | |
0.0514 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA74-80 | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate | - |
Plasmodium falciparum | diphosphate + 7,8-dihydropteroate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHPS | - |
Plasmodium falciparum |
dihydropteroate synthase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA74-80 | Plasmodium falciparum | |
0.035 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA257-306 | Plasmodium falciparum | |
0.068 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA247-306 | Plasmodium falciparum | |
0.069 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | wild type | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.08 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA247-306 | Plasmodium falciparum | |
4.7 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | wild type | Plasmodium falciparum | |
5.86 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA257-306 | Plasmodium falciparum | |
6.23 | - |
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | mutant DELTA74-80 | Plasmodium falciparum |