BRENDA - Enzyme Database show
show all sequences of 2.4.99.20

ADP-ribosyl cyclase and CD38 catalyze the synthesis of a calcium-mobilizing metabolite from NADP+

Aarhus, R.; Graeff, R.M.; Dickey, D.M.; Walseth, T.F.; Lee, H.C.; J. Biol. Chem. 270, 30327-30333 (1995)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5
-
nicotinate
pH 5, 22C
Aplysia californica
5
-
nicotinate
pH 5, 22C
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aplysia californica
P29241
bifunctional enzyme that can synthesize cADPR from NAD1 as well as hydrolyze cADPR to ADP-ribose
-
Homo sapiens
P28907
bifunctional enzyme that can synthesize cADPR from NAD1 as well as hydrolyze cADPR to ADP-ribose
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-phospho-cyclic ADP-ribose + nicotinate
-
727816
Homo sapiens
nicotinate-adenine dinucleotide phosphate
-
-
-
?
2'-phospho-cyclic ADP-ribose + nicotinate
-
727816
Aplysia californica
nicotinate-adenine dinucleotide phosphate
-
-
-
?
additional information
in the absence of nicotinic acid or at neutral pH, isoform CD38 converts NADP+ to ADP-ribose 2'-phosphate, which is the hydrolysis product of cyclic ADPribose 2'-phosphate, reaction of EC 3.2.2.6
727816
Aplysia californica
?
-
-
-
-
additional information
the switch of the catalysis to the exchange reaction requires acidic pH and nicotinic acid. In the absence of nicotinic acid or at neutral pH, the cyclase converts NADP+ to cyclic ADP-ribose 2'-phosphate
727816
Homo sapiens
?
-
-
-
-
NADP+
-
727816
Homo sapiens
2'-phospho-cyclic ADP-ribose + nicotinamide
-
-
-
?
NADP+
-
727816
Aplysia californica
2'-phospho-cyclic ADP-ribose + nicotinamide
-
-
-
?
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5
-
nicotinate
pH 5, 22C
Aplysia californica
5
-
nicotinate
pH 5, 22C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2'-phospho-cyclic ADP-ribose + nicotinate
-
727816
Homo sapiens
nicotinate-adenine dinucleotide phosphate
-
-
-
?
2'-phospho-cyclic ADP-ribose + nicotinate
-
727816
Aplysia californica
nicotinate-adenine dinucleotide phosphate
-
-
-
?
additional information
in the absence of nicotinic acid or at neutral pH, isoform CD38 converts NADP+ to ADP-ribose 2'-phosphate, which is the hydrolysis product of cyclic ADPribose 2'-phosphate, reaction of EC 3.2.2.6
727816
Aplysia californica
?
-
-
-
-
additional information
the switch of the catalysis to the exchange reaction requires acidic pH and nicotinic acid. In the absence of nicotinic acid or at neutral pH, the cyclase converts NADP+ to cyclic ADP-ribose 2'-phosphate
727816
Homo sapiens
?
-
-
-
-
NADP+
-
727816
Homo sapiens
2'-phospho-cyclic ADP-ribose + nicotinamide
-
-
-
?
NADP+
-
727816
Aplysia californica
2'-phospho-cyclic ADP-ribose + nicotinamide
-
-
-
?
Other publictions for EC 2.4.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727515
Schmid
CD38: a NAADP degrading enzyme ...
Homo sapiens, Mus musculus
FEBS Lett.
585
3544-3548
2011
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2
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2
2
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727868
Rah
Generation of cyclic ADP-ribos ...
Mus musculus
J. Biol. Chem.
285
21877-21887
2010
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1
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1
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1
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1
1
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726853
Moreschi
NAADP+ synthesis from cADPRP a ...
Aplysia californica, Axinella polypoides, Homo sapiens
Biochem. Biophys. Res. Commun.
345
573-580
2006
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3
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9
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9
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727857
Graeff
Acidic residues at the active ...
Homo sapiens
J. Biol. Chem.
281
28951-28957
2006
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1
1
5
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1
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1
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1
5
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726898
Bacher
Channelling of substrate promi ...
Oryctolagus cuniculus
Biochem. J.
381
147-154
2004
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6
1
2
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1
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4
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5
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5
6
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1
2
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4
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724210
Chini
CD38 is the major enzyme respo ...
Mus musculus, Rattus norvegicus
Biochem. J.
362
125-130
2002
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4
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2
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2
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3
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7
4
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6
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1
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1
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4
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2
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7
4
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6
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1
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1
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3
3
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726647
Lee
ADP-ribosyl cyclase and CD38. ...
Aplysia californica, Homo sapiens
Adv. Exp. Med. Biol.
419
411-419
1997
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1
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2
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8
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1
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8
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727816
Aarhus
ADP-ribosyl cyclase and CD38 c ...
Aplysia californica, Homo sapiens
J. Biol. Chem.
270
30327-30333
1995
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2
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2
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6
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2
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6
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