BRENDA - Enzyme Database
show all sequences of 2.4.1.87

Structural basis of UDP-galactose binding by alpha-1,3-galactosyltransferase (alpha3GT): role of negative charge on aspartic acid 316 in structure and activity

Tumbale, P.; Jamaluddin, H.; Thiyagarajan, N.; Brew, K.; Acharya, K.R.; Biochemistry 47, 8711-8718 (2008)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
crystal structure of the Glu317Gln mutant in complex with UDP-Gal is reported; purified recombinant enzyme mutants with bound UDP-Gal and Mn2+, hanging drop vapour diffusion at 16°C, mixing of 0.001 ml protein solution containing 5 mg/ml mutant E317Q in 20 mM MES-NaOH buffer, pH 6.0, and 10% glycerol, containing 10 mM MnCl2 and 10 mM UDP-Gal with 0.001 ml of reservoir solution containing 10% PEG 6000, 0.1 M Tris-HCl, pH 8.0, and 8% MPD, for mutant D316N and D316E, 0.001 ml of 5 mg/ml protein in in 20 mM MES-NaOH buffer, pH 6.0, with 10% glycerol, 10 mM UDP-Gal, 10 mM MnCl2, and 10 mM N-acetyllactosamine, are mixed with 0.001 ml of a reservoir solution containing 5-10% PEG 6000, 0.1 M Tris-HCl, pH 8.0, and 5-14% MPD, X-ray diffraction structure determination and analysis at 1.77-2.2 A resolution
Bos taurus
Engineering
Amino acid exchange
Commentary
Organism
D316E
mutant show modest reduction in kcat and Km for lactose. Strucutural studies with mutant D316E show that the negative charge is crucial for catalytic activity and needed for its interaction with Arg202 for an active site structure that facilitates the binding of UDP-gal in a catalytically competent conformation; site-directed mutagenesis, a catalytic domain mutant, crystal structure determination with bound UDP-Gal and Mn2+
Bos taurus
D316N
mutant is inactive. Strucutural studies with mutant D316N show that the negative charge is crucial for catalytic activity and needed for its interaction with Arg202 for an active site structure that facilitates the binding of UDP-gal in a catalytically competent conformation; site-directed mutagenesis, a catalytic domain mutant, crystal structure determination with bound UDP-Gal and Mn2+
Bos taurus
E317Q
crystal structure of the complex of mutant E317Q with UDP-galactose exhibiting a bent configuration stabilized by interactions of the galactose with multiple residues in the enzyme including those in a highly conserved region (His315 to Ser318) is shown; site-directed mutagenesis, a catalytic domain mutant, crystal structure determination with bound UDP-Gal and Mn2+
Bos taurus
H315Q
mutant protein shows modest changes in kinetic parameters for lactose
Bos taurus
H315R
mutant shows a major losss in catalytic activity arising from a 500fold reduction in kcat
Bos taurus
H319A
mutant shows a 2fold reduction for kcat
Bos taurus
H319E
mutant is inactive
Bos taurus
H319Y
mutant is inactive
Bos taurus
S318A
mutant shows a 10fold reduction for kcat
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
binding structure at the catalytic domain, structure model, overview
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Bos taurus
P14769
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
structural basis of UDP-galactose binding at the catalytic domain of alpha3GT, overview
690994
Bos taurus
?
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-
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structure of the Glu317Gln mutant in complex with UDP-Gal is reported
Bos taurus
purified recombinant enzyme mutants with bound UDP-Gal and Mn2+, hanging drop vapour diffusion at 16°C, mixing of 0.001 ml protein solution containing 5 mg/ml mutant E317Q in 20 mM MES-NaOH buffer, pH 6.0, and 10% glycerol, containing 10 mM MnCl2 and 10 mM UDP-Gal with 0.001 ml of reservoir solution containing 10% PEG 6000, 0.1 M Tris-HCl, pH 8.0, and 8% MPD, for mutant D316N and D316E, 0.001 ml of 5 mg/ml protein in in 20 mM MES-NaOH buffer, pH 6.0, with 10% glycerol, 10 mM UDP-Gal, 10 mM MnCl2, and 10 mM N-acetyllactosamine, are mixed with 0.001 ml of a reservoir solution containing 5-10% PEG 6000, 0.1 M Tris-HCl, pH 8.0, and 5-14% MPD, X-ray diffraction structure determination and analysis at 1.77-2.2 A resolution
Bos taurus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D316E
mutant show modest reduction in kcat and Km for lactose. Strucutural studies with mutant D316E show that the negative charge is crucial for catalytic activity and needed for its interaction with Arg202 for an active site structure that facilitates the binding of UDP-gal in a catalytically competent conformation
Bos taurus
D316E
site-directed mutagenesis, a catalytic domain mutant, crystal structure determination with bound UDP-Gal and Mn2+
Bos taurus
D316N
mutant is inactive. Strucutural studies with mutant D316N show that the negative charge is crucial for catalytic activity and needed for its interaction with Arg202 for an active site structure that facilitates the binding of UDP-gal in a catalytically competent conformation
Bos taurus
D316N
site-directed mutagenesis, a catalytic domain mutant, crystal structure determination with bound UDP-Gal and Mn2+
Bos taurus
E317Q
crystal structure of the complex of mutant E317Q with UDP-galactose exhibiting a bent configuration stabilized by interactions of the galactose with multiple residues in the enzyme including those in a highly conserved region (His315 to Ser318) is shown
Bos taurus
E317Q
site-directed mutagenesis, a catalytic domain mutant, crystal structure determination with bound UDP-Gal and Mn2+
Bos taurus
H315Q
mutant protein shows modest changes in kinetic parameters for lactose
Bos taurus
H315R
mutant shows a major losss in catalytic activity arising from a 500fold reduction in kcat
Bos taurus
H319A
mutant shows a 2fold reduction for kcat
Bos taurus
H319E
mutant is inactive
Bos taurus
H319Y
mutant is inactive
Bos taurus
S318A
mutant shows a 10fold reduction for kcat
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
binding structure at the catalytic domain, structure model, overview
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
structural basis of UDP-galactose binding at the catalytic domain of alpha3GT, overview
690994
Bos taurus
?
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Other publictions for EC 2.4.1.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736317
Ardevol
Reaction mechanisms in carbohy ...
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J. Am. Chem. Soc.
137
7528-7547
2015
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4
4
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736808
Park
Comparative n-linked glycan an ...
Sus scrofa
Mol. Cells
38
65-74
2015
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736656
Galili
Significance of the evolutiona ...
Ateles sp., Callithrix jacchus, Cercopithecoidea, Chlorocebus sabaeus, Erythrocebus patas, Eutheria, Gorilla gorilla, Hominoidea, Homo sapiens, Lemuridae, Macaca mulatta, Metatheria, Pan troglodytes, Platyrrhini, Pongo abelii, Pongo pygmaeus, Saimiri sp.
J. Mol. Evol.
80
1-9
2014
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7
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17
17
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736308
Gomez
Substrate-assisted and nucleop ...
Bos taurus
J. Am. Chem. Soc.
135
7053-7063
2013
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2
2
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721667
Han
The wciN gene encodes an alpha ...
Streptococcus pneumoniae
Biochemistry
51
5804-5810
2012
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6
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722792
Ohashi
Identification of novel alpha1 ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe ARC039
J. Biol. Chem.
287
38866-38875
2012
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707367
Jamaluddin
Crystal structure of alpha-1,3 ...
Bos taurus
Biochem. Biophys. Res. Commun.
385
601-604
2009
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4
707599
Pasek
Bioconjugation and detection o ...
Bos taurus
Bioconjug. Chem.
20
608-618
2009
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1
1
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9
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20
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1
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6
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5
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10
10
690994
Tumbale
Structural basis of UDP-galact ...
Bos taurus
Biochemistry
47
8711-8718
2008
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9
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12
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1
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693460
Saethre
Cytokine secretion depends on ...
Sus scrofa
J. Immunol.
180
6346-6353
2008
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708604
Tumbale
Screening a limited structure- ...
Bos taurus
Glycobiology
18
1036-1043
2008
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1
1
1
8
1
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12
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708606
Shoemaker
Temperature-dependent cooperat ...
Homo sapiens
Glycobiology
18
587-592
2008
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672885
Hellrung
Co-expression of alpha(1,3)gal ...
Mus musculus
Cancer Immunol. Immunother.
56
25-34
2007
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1
1
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675442
Jamaluddin
Conformational changes induced ...
Bos taurus
J. Mol. Biol.
369
1270-1281
2007
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676897
Koike
Functionally important glycosy ...
Alouatta caraya, Lemur catta, Loris tardigradus
Proc. Natl. Acad. Sci. USA
104
559-564
2007
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691043
Molina
Site-directed mutagenesis of g ...
Bos taurus
Biochim. Biophys. Acta
1770
1266-1273
2007
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4
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675197
Milland
The molecular basis for galalp ...
Mus musculus
J. Immunol.
176
2448-2454
2006
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672883
Deriy
In vivo targeting of vaccinati ...
Mus musculus
Cancer Gene Ther.
12
528-539
2005
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658845
Zhang
Roles of active site tryptopha ...
Bos taurus
Glycobiology
14
1295-1302
2004
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1
5
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657968
Zhang
Roles of individual enzyme-sub ...
Bos taurus
Biochemistry
42
13512-13521
2003
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1
4
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14
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2
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2
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12
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1
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1
4
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14
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-
-
2
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
658837
Taylor
Characterization of the rat al ...
Rattus norvegicus
Glycobiology
13
327-337
2003
-
-
1
-
-
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-
-
-
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1
-
4
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-
489479
Mercier
Regulation of alpha1,3galactos ...
Sus scrofa
Eur. J. Biochem.
269
1464-1473
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
2
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489477
Imberty
Fold recognition study of alph ...
Sus scrofa
Glycobiology
9
713-722
1999
-
-
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489478
Stults
Characterization of the substr ...
Mus musculus
Glycobiology
9
661-668
1999
1
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489475
Seppo
Enzymic synthesis of octadecam ...
Bos taurus, Mus musculus
Biochemistry
34
4655-4661
1995
-
2
-
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489474
Henion
Defining the minimal size of c ...
Callithrix jacchus, no activity in african green monkey
Glycobiology
4
193-201
1994
-
-
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-
489476
Meldgaard
Blood group ABO-related glycos ...
Homo sapiens
Cancer Res.
54
2440-2447
1994
-
-
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-
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-
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1
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-
-
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-
-
-
-
-
-
-
-
-
-
-
489469
Joziasse
Murine alpha 1,3-galactosyltra ...
Mus musculus
J. Biol. Chem.
267
5534-5541
1992
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
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489468
Elices
Biosynthesis of bi-, tri-, and ...
Mus musculus
J. Biol. Chem.
264
1375-1380
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
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-
-
-
-
-
489470
Joziasse
Bovine alpha 1,3-galactosyltra ...
Bos taurus
J. Biol. Chem.
264
14290-14297
1989
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
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-
489465
Elices
Glycosyltransferase activities ...
Bos taurus, Mus musculus
Arch. Biochem. Biophys.
254
329-341
1987
-
-
-
-
-
-
3
-
2
4
-
2
-
2
-
-
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-
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-
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-
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-
-
489471
Elices
Purification and characterizat ...
Mus musculus
J. Biol. Chem.
261
6064-6072
1986
1
-
-
-
-
1
-
5
1
1
1
-
-
1
-
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1
1
-
-
-
-
-
-
-
-
489466
Blanken
Biosynthesis of terminal Gal a ...
Bos taurus
J. Biol. Chem.
260
12927-12934
1985
-
-
-
-
-
-
-
5
-
-
1
-
-
1
-
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-
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-
-
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-
-
489464
Van den Eijnden
Identification and characteriz ...
Bos taurus
Eur. J. Biochem.
134
523-530
1983
1
-
-
-
-
-
1
3
1
2
-
2
-
1
-
-
-
-
-
1
-
-
7
-
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489472
Van Halbeek
alpha-D-Galactosyltransferase ...
Bos taurus
Biochem. Biophys. Res. Commun.
110
124-131
1983
-
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-
-
-
-
-
-
-
489473
Van den Eijnden
Novikoff ascites tumor cells c ...
Rattus norvegicus
J. Biol. Chem.
258
3435-3437
1983
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
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-
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-
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-
-
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-
-
-
-
-
-
-
-
-
489463
Blake
An alpha-D-galactosyltransfera ...
Mus musculus
J. Biol. Chem.
256
5387-5393
1981
-
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
1
-
-
1
-
-
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-
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-
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-
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-
-
-
-
-
-
-
489467
Beyer
Glycosyltransferases and their ...
Bos taurus
Adv. Enzymol. Relat. Areas Mol. Biol.
52
23-175
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
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-
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-
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-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
489462
Basu
Enzymatic synthesis of a blood ...
Oryctolagus cuniculus
J. Biol. Chem.
248
1700-1706
1973
-
-
-
-
-
-
1
2
-
1
-
2
-
2
-
-
1
-
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3
-
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