Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | stimulates at low concentrations | Dictyostelium discoideum | |
fucose | stimulates | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | complete inhibition of activity with lacto-N-biose I | Dictyostelium discoideum | |
NEM | - |
Dictyostelium discoideum | |
Triton X-100 | - |
Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00127 | - |
GDP-L-fucose | - |
Dictyostelium discoideum | |
2.8 | - |
lacto-N-biose I | - |
Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Dictyostelium discoideum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | strong stimulation by Mg2+ and Mn2+ | Dictyostelium discoideum | |
Mn2+ | strong stimulation by Mg2+ and Mn2+ | Dictyostelium discoideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + beta-D-galactosyl-R | - |
Dictyostelium discoideum | GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R | - |
? | |
GDP-L-fucose + lacto-N-biose 1 | - |
Dictyostelium discoideum | GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
- |
Dictyostelium discoideum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
16 | 30 | 16°C: 61% of maximal activity, 30°C: 62% of maximal activity | Dictyostelium discoideum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
complete inhibition by heating at 100°C | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Dictyostelium discoideum |