BRENDA - Enzyme Database
show all sequences of 2.4.1.47

UDPgalactose: ceramide galactosyltransferase from rat brain

Neskovic, N.M.; Mandel, P.; Gatt, S.; Methods Enzymol. 71, 521-527 (1981)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
phosphatidylcholine
stimulates
Rattus norvegicus
phosphatidylethanolamine
stimulates
Rattus norvegicus
Phospholipids
addition to incubation mixture containing Triton X-100 increases activity
Rattus norvegicus
General Stability
General Stability
Organism
glycerol stabilizes
Rattus norvegicus
in presence of glycerol the enzyme can be frozen and rethawed several times without appreciable loss of stability
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Detergents
inhibition by excess detergent
Rattus norvegicus
DL-sphingosine
-
Rattus norvegicus
Octanoyl-D-threo-p-nitro-1-phenyl-2-amino-1,3-propanediol
-
Rattus norvegicus
Protein inhibitor
from brain, kidney, spleen and liver of rat and other animals
Rattus norvegicus
sodium deoxycholate
strong inhibition
Rattus norvegicus
sodium taurocholate
strong inhibition
Rattus norvegicus
sodium taurodeoxycholate
strong inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
2-(2-hydroxyacyl)sphingosine
Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM
Rattus norvegicus
0.027
0.034
UDPgalactose
-
Rattus norvegicus
0.15
-
2-(2-hydroxyacyl)sphingosine
Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
400000
500000
lipoprotein complex
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
15-20 days old
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
lipoprotein
-
Rattus norvegicus
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.002733
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20C, 50% w/v glycerol, 1 month, about 30% loss of activity
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
not: glucosylceramide, lactosylceramide, asialo GM2ganglioside
489216
Rattus norvegicus
?
-
-
-
-
additional information
not: ceramide containing nonhydroxy fatty acids
489216
Rattus norvegicus
?
-
-
-
-
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
cerebroside
489216
Rattus norvegicus
?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
2-hydroxy fatty acid cerebroside
489216
Rattus norvegicus
?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
i.e. 2-hydroxy fatty acid galactosylceramide
489216
Rattus norvegicus
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
27
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8.2
-
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
phosphatidylcholine
stimulates
Rattus norvegicus
phosphatidylethanolamine
stimulates
Rattus norvegicus
Phospholipids
addition to incubation mixture containing Triton X-100 increases activity
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
glycerol stabilizes
Rattus norvegicus
in presence of glycerol the enzyme can be frozen and rethawed several times without appreciable loss of stability
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Detergents
inhibition by excess detergent
Rattus norvegicus
DL-sphingosine
-
Rattus norvegicus
Octanoyl-D-threo-p-nitro-1-phenyl-2-amino-1,3-propanediol
-
Rattus norvegicus
Protein inhibitor
from brain, kidney, spleen and liver of rat and other animals
Rattus norvegicus
sodium deoxycholate
strong inhibition
Rattus norvegicus
sodium taurocholate
strong inhibition
Rattus norvegicus
sodium taurodeoxycholate
strong inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
2-(2-hydroxyacyl)sphingosine
Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM
Rattus norvegicus
0.027
0.034
UDPgalactose
-
Rattus norvegicus
0.15
-
2-(2-hydroxyacyl)sphingosine
Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
400000
500000
lipoprotein complex
Rattus norvegicus
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
lipoprotein
-
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.002733
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 50% w/v glycerol, 1 month, about 30% loss of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
not: glucosylceramide, lactosylceramide, asialo GM2ganglioside
489216
Rattus norvegicus
?
-
-
-
-
additional information
not: ceramide containing nonhydroxy fatty acids
489216
Rattus norvegicus
?
-
-
-
-
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
cerebroside
489216
Rattus norvegicus
?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
2-hydroxy fatty acid cerebroside
489216
Rattus norvegicus
?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
i.e. 2-hydroxy fatty acid galactosylceramide
489216
Rattus norvegicus
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
27
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8.2
-
Rattus norvegicus
Other publictions for EC 2.4.1.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703918
Niimura
Modification of sphingoglycoli ...
Canis lupus familiaris, Chlorocebus sabaeus
Glycobiology
20
711-717
2010
1
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-
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3
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4
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2
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-
2
-
-
662680
Fewou
Reversal of non-hydroxy:alpha- ...
Rattus norvegicus
J. Neurochem.
94
469-481
2005
-
-
-
-
1
-
-
-
-
-
-
-
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1
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1
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1
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662811
Sprong
Association of the Golgi UDP-g ...
Rattus norvegicus
Mol. Biol. Cell
14
3482-3493
2003
-
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-
1
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1
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1
1
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2
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2
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489224
Tencomnao
Characterization of the human ...
Homo sapiens
Biochim. Biophys. Acta
1517
416-423
2001
-
-
1
-
-
-
-
-
-
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1
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1
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3
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2
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1
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1
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3
-
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2
-
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-
-
-
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-
-
-
-
-
-
-
-
489221
Kapitonov
Cloning, characterization, and ...
Gallus gallus, Homo sapiens
Biochem. Biophys. Res. Commun.
232
449-453
1997
-
1
2
-
-
-
-
-
1
-
-
1
-
5
-
1
-
-
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2
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3
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2
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1
2
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1
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1
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1
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2
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3
-
2
-
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-
-
-
-
-
-
-
-
-
-
489220
Bosio
Molecular cloning and characte ...
Homo sapiens, Mus musculus, Rattus norvegicus
Genomics
35
223-226
1996
-
-
3
-
-
-
-
-
4
-
1
3
-
4
-
2
1
-
-
2
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
1
3
-
-
2
1
-
2
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489222
Bosio
The human gene CGT encoding th ...
Homo sapiens
Genomics
34
69-75
1996
-
-
1
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-
-
-
-
2
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1
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2
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1
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2
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1
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2
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1
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1
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2
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489225
Schulte
UDP galactose:ceramide galacto ...
Rattus norvegicus
Eur. J. Biochem.
233
947-953
1995
-
-
1
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-
-
-
-
2
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2
2
-
1
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1
1
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3
1
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4
1
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1
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2
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2
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1
1
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3
1
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4
1
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-
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489223
Padmini
UDP galactose:ceramide galacto ...
Rattus norvegicus
J. Neurosci. Res.
23
310-315
1989
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1
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1
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1
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4
1
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1
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2
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489219
Berardi
-
Purification and characterizat ...
Rattus norvegicus
NATO ASI Ser. A, Life Sci.
150
681-687
1988
3
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1
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1
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489216
Neskovic
UDPgalactose: ceramide galacto ...
Rattus norvegicus
Methods Enzymol.
71
521-527
1981
3
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-
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2
7
3
1
-
1
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2
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1
1
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2
1
1
5
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1
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1
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3
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2
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7
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3
1
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1
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1
1
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2
1
1
5
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1
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1
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489217
Constantino-Ceccarini
A novel assay method for the b ...
Rattus norvegicus
Methods Enzymol.
72
384-391
1981
-
-
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1
1
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2
2
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1
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1
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1
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1
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489218
Neskovic
Brain UDPgalactose: ceramide g ...
Rattus norvegicus
Biochim. Biophys. Acta
429
342-351
1976
-
-
-
-
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2
2
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2
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1
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1
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1
1
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1
1
2
1
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2
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2
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2
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1
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1
1
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1
1
2
1
-
-
-
-
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-
-
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-
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-
489215
Basu
Enzymatic synthesis of galacto ...
Cavia porcellus, Gallus gallus, Rattus norvegicus, Sus scrofa
J. Biol. Chem.
246
4272-4279
1971
-
-
-
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1
2
2
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6
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2
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4
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5
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1
8
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2
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1
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1
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2
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2
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6
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2
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5
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1
8
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2
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1
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489214
Morell
Synthesis of cerebroside by br ...
Mus musculus
Biochemistry
8
506-512
1969
-
-
-
-
-
-
-
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1
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1
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1
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1
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6
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1
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1
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1
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6
-
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-
489240
Fujino
Enzymic synthesis of cerebrosi ...
Rattus norvegicus
Biochem. J.
113
573-575
1969
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2
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1
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1
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2
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1
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