Cloned (Comment) | Organism |
---|---|
gene gtA, functional recombinant expression of FLAG-tagged GntA enzyme in Saccharomyces cerevisiae | Aspergillus fumigatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Aspergillus fumigatus | 5794 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant FLAG-tagged GntA, SDS-PAGE | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
gene gntA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | two putative N-glycosylation sites at Asn66 and Asn243. The two predicted N-glycosylation sites are located on each side of the catalytically relevant DXD motif | Aspergillus fumigatus |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant FLAG-tagged GntA, SDS-PAGE | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
GntA | - |
Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
malfunction | targeted deletion of the gene encoding GntA in A. fumigatus results in complete absence of zwitterionic glycoinositolphosphoceramide, a phenotype that can be reverted by episomal expression of GntA in the mutant | Aspergillus fumigatus |
physiological function | the enzyme is involved in the initiation of zwitterionic glycoinositolphosphoceramide biosynthesis. Glycoinositolphosphoceramides (GIPCs) are complex sphingolipids that, in fungi, commonly contain an alpha-mannose residue linked at position 2 of the inositol. Several pathogenic fungi additionally synthesize zwitterionic GIPCs carrying an alpha-glucosamine residue at this position. In the human pathogen Aspergillus fumigatus, the alpha-N-acetylglucosaminyl-1,2-inositolphosphoceramide core is elongated to Manalpha1,3Manalpha1,6GlcNalpha1,2IPC, which is the most abundant glycoinositolphosphoceramide synthesized by this fungus. GntA uses UDP-N-acetylglucosamine as donor substrate to generate a glycolipid product resistant to saponification and to digestion by phosphatidylinositol-phospholipase C as expected for GlcNAcalpha1,2IPC | Aspergillus fumigatus |