Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli as glutathione-S-transferase fusion protein, wild-type and mutant enzymes: L263M, F265Y, G256A, S338G, L263M/F265Y, G256A/S338G, L263M/S338G, F265Y/S338G, L263M/F265Y/S338G, G256A/L263M/F265Y, G256A/L263M/F265Y/S338G | Hypericum androsaemum |
Protein Variants | Comment | Organism |
---|---|---|
F265Y | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 7.2:1 for the mutant enzyme | Hypericum androsaemum |
F265Y/S338G | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 2.2:1 for the mutant enzyme | Hypericum androsaemum |
G256A | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 4.0:1 for the mutant enzyme | Hypericum androsaemum |
G256A/L263M/F265Y | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 12.5:1 for the mutant enzyme | Hypericum androsaemum |
G256A/L263M/F265Y/S338G | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 1.1:1 for the mutant enzyme | Hypericum androsaemum |
G256A/S338G | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 3.0:1 for the mutant enzyme | Hypericum androsaemum |
L263M | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 4.0:1 for the mutant enzyme | Hypericum androsaemum |
L263M/F265Y | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 3.7:1 for the mutant enzyme | Hypericum androsaemum |
L263M/F265Y/S338G | mutant enzyme preferres benzoyl-CoA over 4-coumaroyl-CoA,relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 1.0:1.9 for the mutant enzyme | Hypericum androsaemum |
L263M/S338G | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 3.9:1 for the mutant enzyme | Hypericum androsaemum |
S338G | relative activity with 4-coumaroyl-CoA and benzoyl-CoA as starter substrate is 4.5:1 for wild-type enzyme and 4.4:1 for the mutant enzyme | Hypericum androsaemum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
4-coumaroyl-CoA | pH 7.0, 35°C, wild-type enzyme | Hypericum androsaemum | |
0.0054 | - |
benzoyl-CoA | pH 7.0, 35°C, mutant enzyme L263M/F265Y/S338G | Hypericum androsaemum | |
0.0068 | - |
benzoyl-CoA | pH 7.0, 35°C, wild-type enzyme | Hypericum androsaemum | |
0.0078 | - |
4-coumaroyl-CoA | pH 7.0, 35°C, mutant enzyme L263M/F265Y/S338G | Hypericum androsaemum | |
0.0099 | - |
malonyl-CoA | pH 7.0, 35°C, mutant enzyme L263M/F265Y/S338G | Hypericum androsaemum | |
0.0108 | - |
malonyl-CoA | pH 7.0, 35°C, wild-type enzyme | Hypericum androsaemum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hypericum androsaemum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + 3 malonyl-CoA | - |
Hypericum androsaemum | naringenin chalcone + 4 CoA + 3 CO2 | - |
? | |
cinnamoyl-CoA + malonyl-CoA | 87.5% of the activity with 4-coumaroyl-CoA + malonyl-CoA | Hypericum androsaemum | 5,7-dihydroxyflavanone + CoA + CO2 | - |
? | |
malonyl-CoA + 3-coumaroyl-CoA | 34.4% of the activity with 4-coumaroyl-CoA + malonyl-CoA | Hypericum androsaemum | ? + CO2 + CoA | - |
? | |
malonyl-CoA + 3-hydroxybenzoyl-CoA | 21.8% of the activity with 4-coumaroyl-CoA + malonyl-CoA | Hypericum androsaemum | ? + CO2 + CoA | - |
? | |
malonyl-CoA + benzoyl-CoA | 22.3% of the activity with 4-coumaroyl-CoA + malonyl-CoA | Hypericum androsaemum | phlorobenzophenone + CO2 + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chalcone synthase | - |
Hypericum androsaemum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
4-coumaroyl-CoA | pH 7.0, 35°C, mutant enzyme L263M/F265Y/S338G | Hypericum androsaemum | |
0.0113 | - |
benzoyl-CoA | pH 7.0, 35°C, wild-type enzyme | Hypericum androsaemum | |
0.0156 | - |
benzoyl-CoA | pH 7.0, 35°C, mutant enzyme L263M/F265Y/S338G | Hypericum androsaemum | |
0.042 | - |
4-coumaroyl-CoA | pH 7.0, 35°C, wild-type enzyme | Hypericum androsaemum |