Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | also leads to increased formation of by-products | Daucus carota | |
bovine serum albumin | - |
Daucus carota | |
chlorogenic acid | increases eriodictyol formation by 20% | Daucus carota |
General Stability | Organism |
---|---|
withdrawal of 2-mercaptoethanol after ammonium sulfate precipitation leads to increased stability during further purification | Daucus carota |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-coumaroyl-CoA | above 0.01 mM | Daucus carota | |
cerulenin | - |
Daucus carota | |
eriodictyol | - |
Daucus carota | |
eriodictyol chalcone | - |
Daucus carota | |
malonyl-CoA | substrate inhibition above 0.05 mM | Daucus carota | |
naringenin | 50% inhibition of naringenin formation and total inhibition of eriodictyol formation | Daucus carota | |
naringenin chalcone | 50% inhibition of naringenin formation and total inhibition of eriodictyol formation | Daucus carota |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
4-coumaroyl-CoA | - |
Daucus carota | |
0.003 | - |
malonyl-CoA | - |
Daucus carota | |
0.0077 | - |
caffeoyl-CoA | - |
Daucus carota |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + 3 malonyl-CoA | Daucus carota | - |
naringenin chalcone + 4 CoA + 3 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Daucus carota |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Daucus carota | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.162 | - |
- |
Daucus carota |
Storage Stability | Organism |
---|---|
-70°C, decrease of activity in solutions below 1 mg/ml | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + 3 malonyl-CoA | - |
Daucus carota | naringenin chalcone + 4 CoA + 3 CO2 | - |
? | |
caffeoyl-CoA + 3 malonyl-CoA | - |
Daucus carota | eriodictyol + 4 CoA + 3 CO2 | - |
? | |
malonyl-CoA + 4-coumaroyl-CoA | - |
Daucus carota | naringenin chalcone + CoA + CO2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
half-life 3 h in 1.4 mM 2-mercaptoethanol, increase of stability with 14 mM 2-mercaptoethanol | Daucus carota |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
caffeoyl-CoA | Daucus carota |
7.9 | - |
4-coumaroyl-CoA | Daucus carota |