Literature summary for 2.3.1.266 extracted from

Vetting, M.; Bareich, D.; Yu, M.; Blanchard, J.
Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for Nalpha-acetylation of ribosomal protein S18 (2008), Protein Sci., 17, 1781-1790 .

Search for more literature for 2.3.1.266

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with CoA, acetyl-CoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor, vapor diffusion method, using	Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors	Comment	Organism	Structure
CoA-S-acetyl-ARYFRR	linearly competitive against acetyl-CoA and linearly noncompetitive against S18 protein(1-6)	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]	Salmonella enterica subsp. enterica serovar Typhimurium	-	CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]	-	?

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	Q8ZJW4	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni HiTrap column chromatography	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]	-	Salmonella enterica subsp. enterica serovar Typhimurium	CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]	-	?

Synonyms

Synonyms	Comment	Organism
RimI	-	Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0041	-	CoA-S-acetyl-ARYFRR	at pH 7.3 and 25°C	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)