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Literature summary for 2.3.1.258 extracted from
- From molecular understanding to organismal biology of N-terminal acetyltransferases (2019), Structure, 27, 1053-1055 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression ofthe NatA/Naa50 complex, i.e. NatE, in Escherichia coli | Saccharomyces cerevisiae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| NatA/Naa50 complex, i.e. NatE, including full-length ScNaa15 (residues 1-854), C-terminally truncated ScNaa10 (1-226 out of 238 total residues), and full-length ScNaa50 (residues 1-176), in the presence of inositol hexaphosphate (IP6) and bi-substrate analogues for both Naa10 and Naa50, X-ray diffraction structure determination and analysis | Saccharomyces cerevisiae |
| NatA/Naa50 complex, i.e. NatE, X-ray diffraction structure determination and analysis | Schizosaccharomyces pombe |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | N-terminal analyses comparing wild-type and scNaa50 deletion strains of Saccharomyces cerevisiae | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Schizosaccharomyces pombe | 5829 | - |
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
| cytosol | - |
Homo sapiens | 5829 | - |
| ribosome | near to | Schizosaccharomyces pombe | 5840 | - |
| ribosome | near to | Saccharomyces cerevisiae | 5840 | - |
| ribosome | near to | Homo sapiens | 5840 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9GZZ1 AND P41227 AND Q9BXJ9 | NatE subunits Naa50,Naa10, and Naa15 | - |
| Saccharomyces cerevisiae | Q08689 AND P41227 AND P12945 | NatE complex subunits Naa50, Naa10 (ARD1), and Naa15 (Nat1) | - |
| Saccharomyces cerevisiae ATCC 204508 | Q08689 AND P41227 AND P12945 | NatE complex subunits Naa50, Naa10 (ARD1), and Naa15 (Nat1) | - |
| Schizosaccharomyces pombe | - |
- |
- |
| Schizosaccharomyces pombe 972 | - |
- |
- |
| Schizosaccharomyces pombe ATCC 24843 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | N-terminal acetylation (NTA) is an irreversible protein modification | Schizosaccharomyces pombe | ? | - |
- |
 |
| additional information | N-terminal acetylation (NTA) is an irreversible protein modification | Saccharomyces cerevisiae | ? | - |
- |
|
| additional information | N-terminal acetylation (NTA) is an irreversible protein modification | Homo sapiens | ? | - |
- |
|
| additional information | N-terminal acetylation (NTA) is an irreversible protein modification | Schizosaccharomyces pombe ATCC 24843 | ? | - |
- |
|
| additional information | N-terminal acetylation (NTA) is an irreversible protein modification | Schizosaccharomyces pombe 972 | ? | - |
- |
|
| additional information | N-terminal acetylation (NTA) is an irreversible protein modification | Saccharomyces cerevisiae ATCC 204508 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the NatE enzyme complex is composed of the subunits Naa50 and Naa15 | Schizosaccharomyces pombe |
| More | the NatE enzyme complex is composed of the subunits Naa50 and Naa15 | Saccharomyces cerevisiae |
| More | the NatE enzyme complex is composed of the subunits Naa50, Naa10, and Naa15 | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ARD1 | - |
Schizosaccharomyces pombe |
| ARD1 | - |
Saccharomyces cerevisiae |
| NAA10 | - |
Schizosaccharomyces pombe |
| NAA10 | - |
Saccharomyces cerevisiae |
| NAA10 | - |
Homo sapiens |
| NAA15 | - |
Schizosaccharomyces pombe |
| NAA15 | - |
Saccharomyces cerevisiae |
| NAA15 | - |
Homo sapiens |
| Naa50 | - |
Schizosaccharomyces pombe |
| Naa50 | - |
Saccharomyces cerevisiae |
| Naa50 | - |
Homo sapiens |
| NAT1 | - |
Schizosaccharomyces pombe |
| NAT1 | - |
Saccharomyces cerevisiae |
| NAT5 | UniProt | Schizosaccharomyces pombe |
| NAT5 | UniProt | Saccharomyces cerevisiae |
| NAT5 | UniProt | Homo sapiens |
| NatE | - |
Schizosaccharomyces pombe |
| NatE | - |
Saccharomyces cerevisiae |
| NatE | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Schizosaccharomyces pombe | |
| acetyl-CoA | - |
Saccharomyces cerevisiae | |
| acetyl-CoA | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | there are seven known NAT types (NatA through NatG), each composed of one or more specific subunits and having specific substrates defined by the very first amino acid residue (serine, alanine, etc.) | Homo sapiens |
| evolution | there are seven known NAT types (NatA through NatG), each composed of one or more specific subunits and having specific substrates defined by the very first amino acid residue (serine, alanine, etc.). SpNaa50 and ScNaa50 do not contain an optimal Q/RxxGxG/A consensus acetyl-CoA binding motif | Schizosaccharomyces pombe |
| evolution | there are seven known NAT types (NatA through NatG), each composed of one or more specific subunits and having specific substrates defined by the very first amino acid residue (serine, alanine, etc.). SpNaa50 and ScNaa50 do not contain an optimal Q/RxxGxG/A consensus acetyl-CoA binding motif | Saccharomyces cerevisiae |
| metabolism | the enzyme is involved in the co-translational N-terminal protein modification process, overview | Schizosaccharomyces pombe |
| metabolism | the enzyme is involved in the co-translational N-terminal protein modification process, overview | Saccharomyces cerevisiae |
| metabolism | the enzyme is involved in the co-translational N-terminal protein modification process, overview | Homo sapiens |
| additional information | the NatE enzyme complex is composed of the subunits Naa50 and Naa15 | Schizosaccharomyces pombe |
| additional information | the NatE enzyme complex is composed of the subunits Naa50 and Naa15 | Saccharomyces cerevisiae |
| additional information | the NatE enzyme complex is composed of the subunits Naa50, Naa10, and Naa15 | Homo sapiens |
| physiological function | N-terminal acetylation (NTA) is among the most widespread co-translational modifications found in eukaryotic proteins. NTA is carried out by N-terminal acetyltransferases (NATs), which catalyze the transfer of an acetyl moiety from acetyl coenzyme A to the N-terminal amino group of the nascent polypeptides as they emerge from the ribosome. NTA is an irreversible protein modification | Schizosaccharomyces pombe |
| physiological function | N-terminal acetylation (NTA) is among the most widespread co-translational modifications found in eukaryotic proteins. NTA is carried out by N-terminal acetyltransferases (NATs), which catalyze the transfer of an acetyl moiety from acetyl coenzyme A to the N-terminal amino group of the nascent polypeptides as they emerge from the ribosome. NTA is an irreversible protein modification | Saccharomyces cerevisiae |
| physiological function | N-terminal acetylation (NTA) is among the most widespread co-translational modifications found in eukaryotic proteins. NTA is carried out by N-terminal acetyltransferases (NATs), which catalyze the transfer of an acetyl moiety from acetyl coenzyme A to the N-terminal amino group of the nascent polypeptides as they emerge from the ribosome. NTA is estimated to affect up to 90% of human proteins and influences their folding, localization, complex formation, and degradation, along with a variety of cellular functions ranging from apoptosis to gene regulation. NTA is an irreversible protein modification | Homo sapiens |
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