Crystallization (Comment) | Organism |
---|---|
structure of the 100 kDa holo-NatA complex in the absence and presence of a bisubstrate peptide-CoA conjugate inhibitor, and structure of the uncomplexed Naa10p catalytic subunit. The NatA-Naa15p auxiliary subunit contains 13 TPR motifs and adopts a ring-like topology that wraps around the NatA-Naa10p subunit, an interaction that alters the Naa10p active site for substrate-specific acetylation | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
E24A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
E24D | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
E24Q | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
E61A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
E62A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
H111A | mutation in NatA, decrease in kcat, Km value similar to wild-type | Schizosaccharomyces pombe |
H20A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
H72A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
K29A | mutation in NatA, increase in kcat, decrease in Km value | Schizosaccharomyces pombe |
K29A/Y33A | mutation in NatA, decrease in kcat, Km value similar to wild-type | Schizosaccharomyces pombe |
K59A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
K59A/E61A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
K59A/E62A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
L22A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
P23A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
R113A | mutation in NatA, strong decrease in kcat, Km value similar to wild-type | Schizosaccharomyces pombe |
R80A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
Y139A | mutation in NatA, dramaitc loss of activity | Schizosaccharomyces pombe |
Y26A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
Y33A | mutation in NatA, decrease in kcat, increase in Km value | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonyl-CoA | - |
Schizosaccharomyces pombe | |
CoA-SASEA | CoA covalently linked to substrate peptide fragment Ser1-Ala2-Ser3-Glu4-Ala5 | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
SASEAGVRWGRPVGRRRRP | mutant K29A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.33 | - |
SASEAGVRWGRPVGRRRRP | mutant H111A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.34 | - |
SASEAGVRWGRPVGRRRRP | wild-type, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.34 | - |
SASEAGVRWGRPVGRRRRP | mutant K29A/Y33A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.38 | - |
SASEAGVRWGRPVGRRRRP | mutant R113A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.41 | - |
SASEAGVRWGRPVGRRRRP | mutant E61A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.41 | - |
SASEAGVRWGRPVGRRRRP | mutant K59A/E62A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.44 | - |
SASEAGVRWGRPVGRRRRP | mutant E24Q, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.51 | - |
SASEAGVRWGRPVGRRRRP | mutant K29A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.52 | - |
SASEAGVRWGRPVGRRRRP | mutant K59A/E61A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.59 | - |
SASEAGVRWGRPVGRRRRP | mutant E62A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.6 | - |
SASEAGVRWGRPVGRRRRP | mutant H72A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.73 | - |
SASEAGVRWGRPVGRRRRP | mutant Y26A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.74 | - |
SASEAGVRWGRPVGRRRRP | mutant P23A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.75 | - |
SASEAGVRWGRPVGRRRRP | mutant E24D, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.76 | - |
SASEAGVRWGRPVGRRRRP | mutant K59A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.77 | - |
SASEAGVRWGRPVGRRRRP | mutant R80A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.92 | - |
SASEAGVRWGRPVGRRRRP | mutant H20A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
1.85 | - |
SASEAGVRWGRPVGRRRRP | mutant L22A, pH 8.0, 25°C | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | O74985 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + SASEAGVRWGRPVGRRRRP | - |
Schizosaccharomyces pombe | CoA + Nalpha-acetyl-SASEAGVRWGRPVGRRRRP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAT1 | - |
Schizosaccharomyces pombe |
NatA | - |
Schizosaccharomyces pombe |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
SASEAGVRWGRPVGRRRRP | mutant E24Q, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.047 | - |
SASEAGVRWGRPVGRRRRP | mutant Y26A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.081 | - |
SASEAGVRWGRPVGRRRRP | mutant L22A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.19 | - |
SASEAGVRWGRPVGRRRRP | mutant R113A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.42 | - |
SASEAGVRWGRPVGRRRRP | mutant E24D, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.44 | - |
SASEAGVRWGRPVGRRRRP | mutant Y33A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.61 | - |
SASEAGVRWGRPVGRRRRP | mutant K29A/Y33A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.73 | - |
SASEAGVRWGRPVGRRRRP | mutant K59A/E62A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
0.85 | - |
SASEAGVRWGRPVGRRRRP | mutant R80A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
1.1 | - |
SASEAGVRWGRPVGRRRRP | mutant E61A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
1.1 | - |
SASEAGVRWGRPVGRRRRP | mutant E62A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
1.5 | - |
SASEAGVRWGRPVGRRRRP | mutant H20A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
1.5 | - |
SASEAGVRWGRPVGRRRRP | mutant K59A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
1.7 | - |
SASEAGVRWGRPVGRRRRP | mutant K59A/E61A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
2 | - |
SASEAGVRWGRPVGRRRRP | mutant H111A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
2 | - |
SASEAGVRWGRPVGRRRRP | mutant P23A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
2.4 | - |
SASEAGVRWGRPVGRRRRP | mutant H72A, pH 8.0, 25°C | Schizosaccharomyces pombe | |
3 | - |
SASEAGVRWGRPVGRRRRP | wild-type, pH 8.0, 25°C | Schizosaccharomyces pombe | |
4.4 | - |
SASEAGVRWGRPVGRRRRP | mutant K29A, pH 8.0, 25°C | Schizosaccharomyces pombe |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0014 | - |
pH 8.0, 25°C | Schizosaccharomyces pombe | CoA-SASEA | |
0.38 | - |
pH 8.0, 25°C | Schizosaccharomyces pombe | acetonyl-CoA |