BRENDA - Enzyme Database show
show all sequences of 2.3.1.180

Purification and characterization of 3-ketoacyl-acyl carrier protein synthase III from spinach. A condensing enzyme utilizing acetyl-coenzyme A to initiate fatty acid synthesis

Clough, R.; Matthis, A.L.; Barnum, S.R.; Jaworski, J.G.; J. Biol. Chem. 267, 20992-20998 (1992)

Data extracted from this reference:

General Stability
General Stability
Organism
0.01% Triton X-100 is essential for enzyme stability during purification
Spinacia oleracea
Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
45% inhibition at 5 mM, 98% inhibition at 20 mM
Spinacia oleracea
N-ethylmaleimide
complete inhibition at 5 mM
Spinacia oleracea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40500
-
2 * 40500, SDS-PAGE
Spinacia oleracea
63000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + malonyl-[acyl-carrier protein]
Spinacia oleracea
-
acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
-
-
-
Purification (Commentary)
Commentary
Organism
3500fold to homogeneity from leaves in an eight-step procedure including ACP affinity chromatography
Spinacia oleracea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7
-
purified enzyme
Spinacia oleracea
Storage Stability
Storage Stability
Organism
-80°C, purified enzyme, 20 mM Tris buffer, 1 mM DTT, 20% glycerol, 0.01% Triton X-100, several weeks with slight loss of activity
Spinacia oleracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + malonyl-[acyl-carrier protein]
-
486931
Spinacia oleracea
acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
-
-
r
additional information
substrate specificity, the enzyme also shows acetyltransferase activity, which is lower than the 3-ketoacyl carrier protein synthase activity
486931
Spinacia oleracea
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 40500, SDS-PAGE
Spinacia oleracea
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
assay at
Spinacia oleracea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Spinacia oleracea
Cofactor
Cofactor
Commentary
Organism
Structure
CoA
-
Spinacia oleracea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
CoA
-
Spinacia oleracea
General Stability (protein specific)
General Stability
Organism
0.01% Triton X-100 is essential for enzyme stability during purification
Spinacia oleracea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
45% inhibition at 5 mM, 98% inhibition at 20 mM
Spinacia oleracea
N-ethylmaleimide
complete inhibition at 5 mM
Spinacia oleracea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40500
-
2 * 40500, SDS-PAGE
Spinacia oleracea
63000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + malonyl-[acyl-carrier protein]
Spinacia oleracea
-
acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
3500fold to homogeneity from leaves in an eight-step procedure including ACP affinity chromatography
Spinacia oleracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7
-
purified enzyme
Spinacia oleracea
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, purified enzyme, 20 mM Tris buffer, 1 mM DTT, 20% glycerol, 0.01% Triton X-100, several weeks with slight loss of activity
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + malonyl-[acyl-carrier protein]
-
486931
Spinacia oleracea
acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
-
-
r
additional information
substrate specificity, the enzyme also shows acetyltransferase activity, which is lower than the 3-ketoacyl carrier protein synthase activity
486931
Spinacia oleracea
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40500, SDS-PAGE
Spinacia oleracea
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
assay at
Spinacia oleracea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Spinacia oleracea
Other publictions for EC 2.3.1.180
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2
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1
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2
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1
1
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4
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1
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1
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10
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4
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10
103
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736053
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Eur. J. Med. Chem.
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1
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25
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1
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1
-
1
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1
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1
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1
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1
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1
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13
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1
1
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-
13
25
-
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1
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1
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1
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1
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1
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1
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1
1
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736736
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1
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1
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1
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7
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1
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2
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1
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1
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1
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1
1
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1
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1
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1
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2
-
1
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1
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1
1
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735757
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23
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-
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27
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10
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27
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15
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10
4
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7
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12
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7
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12
36
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9
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10
4
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27
27
-
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736519
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J. Biomol. Struct. Dyn.
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2013
-
1
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1
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2
2
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736551
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1
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3
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1
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1
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2
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2
2
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718478
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-
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1
1
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1
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719068
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22
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1
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13
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719093
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20
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-
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1
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8
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1
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1
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1
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8
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1
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8
8
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1
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720076
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-
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1
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10
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10
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3
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719085
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1
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9
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1
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9
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719086
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-
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1
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7
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1
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7
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7
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719087
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19
4513-4519
2011
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1
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12
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1
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1
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12
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1
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12
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1
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1
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718629
Du
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76
3959-3966
2010
-
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2
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1
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5
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1
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5
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2
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1
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1
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5
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719049
Lv
Design, synthesis, and structu ...
Escherichia coli
Bioorg. Med. Chem. Lett.
20
4657-4660
2010
-
-
-
-
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7
-
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3
-
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7
-
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7
7
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720784
Gonzalez-Mellado
The role of beta-ketoacyl-acyl ...
Helianthus annuus, Helianthus annuus CAS-6
Planta
231
1277-1289
2010
-
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1
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1
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1
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9
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1
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1
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2
1
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1
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1
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1
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1
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1
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1
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2
1
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1
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701521
Huynh
Cloning, expression, crystalli ...
Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzae Xoo KACC10331
Acta Crystallogr. Sect. F
65
460-462
2009
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1
1
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1
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6
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1
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-
-
2
2
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-
-
-
-
-
-
-
-
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-
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1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
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1
1
-
-
-
702531
Matsumoto
Production of short-chain-leng ...
Arabidopsis thaliana, Escherichia coli
Biomacromolecules
10
686-690
2009
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1
-
2
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2
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7
-
-
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1
-
-
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-
-
-
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-
-
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1
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-
2
-
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2
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-
-
-
-
1
-
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-
-
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1
1
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702601
Lv
Design, synthesis and biologic ...
Escherichia coli
Bioorg. Med. Chem. Lett.
19
6750-6754
2009
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1
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28
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8
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1
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8
28
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1
1
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702634
Lee
Novel E. coli beta-ketoacyl-ac ...
Escherichia coli K-12
Bioorg. Med. Chem.
17
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2009
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1
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4
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1
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1
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4
-
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-
-
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-
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-
-
-
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1
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1
1
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702656
Lee
Antimicrobial natural products ...
Escherichia coli K-12
Bioorg. Med. Chem.
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2009
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1
-
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3
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1
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2
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1
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-
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-
1
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-
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-
1
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-
3
-
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-
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-
1
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-
-
-
-
-
1
-
-
-
-
-
1
-
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-
-
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-
-
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702661
Li
Synthesis of C(7) modified chr ...
Escherichia coli
Bioorg. Med. Chem.
17
6264-6269
2009
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1
1
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16
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2
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3
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1
-
-
-
-
-
2
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-
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1
-
-
-
-
-
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-
1
1
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-
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-
16
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
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703762
Singh
FabH selectivity for anteiso b ...
Listeria monocytogenes 10403S, Listeria monocytogenes
FEMS Microbiol. Lett.
301
188-192
2009
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-
-
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7
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9
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13
-
1
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7
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-
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-
-
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-
-
-
-
-
-
-
-
-
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7
-
-
-
9
-
-
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-
-
-
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13
-
1
-
-
7
-
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1
1
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7
7
704622
Veyron-Churlet
The Mycobacterium tuberculosis ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
284
6414-6424
2009
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1
1
2
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2
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11
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1
1
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3
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1
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-
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2
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-
-
-
-
-
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1
-
1
2
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-
-
-
-
-
-
-
2
-
-
1
1
-
-
-
-
3
-
1
-
-
-
2
-
-
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1
1
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705055
Lee
Targeting the fatty acid biosy ...
Plasmodium falciparum
J. Med. Chem.
52
952-963
2009
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1
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43
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1
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3
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1
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43
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1
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43
43
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1
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-
-
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-
-
1
-
-
-
-
-
-
-
-
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1
1
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706427
Al-Balas
Identification of 2-aminothiaz ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
PLoS ONE
4
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2009
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1
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-
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11
-
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10
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-
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6
-
1
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-
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6
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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706592
Wen
Functional expression of Franc ...
Francisella tularensis subsp. holarctica
Protein Expr. Purif.
65
83-91
2009
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1
-
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-
13
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1
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1
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1
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1
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1
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1
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-
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-
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15
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1
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-
15
13
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-
-
-
-
1
-
-
-
1
-
-
-
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1
-
1
-
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1
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1
1
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685507
Sachdeva
Probing reactivity and substra ...
Mycobacterium tuberculosis
Bioorg. Chem.
36
85-90
2008
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-
1
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-
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2
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-
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-
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-
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-
-
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-
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1
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-
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-
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-
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686049
Sachdeva
Separate entrance and exit por ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Chem. Biol.
15
402-412
2008
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1
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-
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9
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1
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-
-
-
-
-
-
-
-
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-
-
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1
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-
-
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-
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-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
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688834
Sachdeva
Mycobacterium tuberculosis bet ...
Mycobacterium tuberculosis
Methods Mol. Med.
142
205-213
2008
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-
-
-
-
-
-
-
-
-
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4
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-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688880
Castillo
Bacterial beta-ketoacyl-acyl c ...
Enterococcus faecium, Escherichia coli, Haemophilus influenzae, Mycobacterium tuberculosis, Neisseria meningitidis, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes
Mini Rev. Med. Chem.
8
36-45
2008
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33
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10
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-
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-
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-
-
-
-
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-
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-
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23
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-
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23
33
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
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690169
Li
Molecular cloning and expressi ...
Jatropha curcas
Tree Physiol.
28
921-927
2008
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1
-
-
-
-
-
-
-
-
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3
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-
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4
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
1
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-
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-
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4
-
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-
-
-
-
-
-
-
-
-
-
-
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-
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703548
Singh
QSAR studies on benzoylaminobe ...
Enterococcus faecalis, Escherichia coli, Neisseria meningitidis, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes
Eur. J. Med. Chem.
43
1071-1080
2008
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276
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6
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12
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6
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276
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6
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6
-
-
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-
-
-
-
-
-
-
-
-
-
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672628
Alhamadsheh
Synthesis and biological evalu ...
Escherichia coli, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Plasmodium falciparum, Plasmodium falciparum D6
Bioorg. Med. Chem. Lett.
17
879-883
2007
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-
-
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46
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15
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-
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3
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47
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-
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47
46
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-
-
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-
-
-
-
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3
-
-
-
-
-
-
-
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676903
Wang
Discovery of platencin, a dual ...
Staphylococcus aureus
Proc. Natl. Acad. Sci. USA
104
7612-7616
2007
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-
-
-
-
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2
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1
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-
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-
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-
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-
1
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1
2
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-
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-
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-
-
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-
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-
-
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685898
Jeong
-
Homology modeling and docking ...
Enterococcus faecalis
Bull. Korean Chem. Soc.
28
1335-1340
2007
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-
-
-
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1
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1
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-
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-
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-
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-
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-
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1
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-
-
-
-
-
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-
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-
-
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686047
Alhamadsheh
Alkyl-CoA disulfides as inhibi ...
Escherichia coli, Mycobacterium tuberculosis
Chem. Biol.
14
513-524
2007
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1
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5
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4
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-
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2
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-
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3
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-
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1
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-
3
5
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-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
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688033
Ashek
HQSAR study of beta-ketoacyl-a ...
Enterococcus faecalis
J. Enzyme Inhib. Med. Chem.
22
7-14
2007
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36
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2
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36
-
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-
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-
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Brown
Probing the mechanism of the M ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
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280
32539-32547
2005
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1
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1
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3
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1
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2
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1
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1
9
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1
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1
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3
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1
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2
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Musayev
Crystal structure of a substra ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
346
1313-1321
2005
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1
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1
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3
1
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-
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-
1
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1
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-
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3
1
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-
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660466
Qiu
Crystal structure and substrat ...
Staphylococcus aureus
Protein Sci.
14
2087-2094
2005
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1
1
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5
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1
1
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4
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1
1
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9
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11
1
1
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8
1
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-
-
-
-
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1
-
1
-
-
-
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5
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1
1
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1
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9
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11
1
1
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8
1
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665387
Li
Alteration of the fatty acid p ...
Streptomyces coelicolor
J. Bacteriol.
187
3795-3799
2005
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1
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6
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4
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-
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-
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1
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2
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4
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665975
Nie
Structure-based design, synthe ...
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1596-1609
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2
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1
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46
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1
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1
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46
59
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1
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2
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1
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663647
He
1,2-dithiole-3-ones as potent ...
Escherichia coli, Staphylococcus aureus
Antimicrob. Agents Chemother.
48
3093-3102
2004
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8
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2
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5
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4
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2
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2
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2
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2
8
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2
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-
4
-
2
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-
2
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663702
Nomura
Coexpression of genetically en ...
Escherichia coli
Appl. Environ. Microbiol.
70
999-1007
2004
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1
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19
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6
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1
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1
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19
-
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-
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1
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-
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1
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659294
Lai
beta-Ketoacyl-acyl carrier pro ...
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278
51494-51503
2003
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2
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1
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7
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1
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1
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-
-
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-
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-
2
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-
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2
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5
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1
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7
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1
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1
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666668
Jones
beta-Ketoacyl-acyl carrier pro ...
Pisum sativum
Planta
216
752-761
2003
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1
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Purification, characterization ...
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1
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664003
Florova
Enzymes involved in fatty acid ...
Streptomyces glaucescens
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Engineered fatty acid biosynth ...
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Khandekar
Identification, substrate spec ...
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663684
Kiatpapan
Molecular characterization of ...
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beta-Ketoacyl acyl carrier pro ...
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Scarsdale
Crystal structure of the Mycob ...
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2001
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Qiu
Refined structures of beta-ket ...
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Overexpression of 3-ketoacyl-a ...
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beta-ketoacyl-acyl carrier pro ...
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Crystallization of Escherichia ...
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Expression, purification, and ...
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Abbadi
Knockout of the regulatory sit ...
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2000
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Davies
The 1.8 A crystal structure an ...
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Bao
Purification and properties of ...
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