BRENDA - Enzyme Database show
show all sequences of 2.3.1.175

The last step in cephalosporin C formation revealed: crystal structures of deacetylcephalosporin C acetyltransferase from Acremonium chrysogenum in complexes with reaction intermediates

Lejon, S.; Ellis, J.; Valegard, K.; J. Mol. Biol. 377, 935-944 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
studies of the enzyme will be useful in gaining a full view of the possibilities for overcoming the acetyl transfer bottleneck and for the future development of cephalosporin antibiotics
Acremonium chrysogenum
Cloned(Commentary)
Commentary
Organism
into the pTWIN vector for expression in Escherichia coli BL21DE3 cells
Acremonium chrysogenum
Crystallization (Commentary)
Crystallization
Organism
different structures are solved, SeMet-DAC-AT with acetyl-CoA to a resolution of 2.4 A, DAC-AT with acetyl-CoA to 2.2 A, DAC-AT with DAC/cephalosporin C to 2.5 A, and DAC-AT with DAC to 2.6 A resolution
Acremonium chrysogenum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + deacetylcephalosporin C
Acremonium chrysogenum
-
CoA + cephalosporin C
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acremonium chrysogenum
P39058
-
-
Purification (Commentary)
Commentary
Organism
using a gravity-flow column containing chitin beads, cleavage of the chitin-binding domain and intein fusion partner is performed
Acremonium chrysogenum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + deacetylcephalosporin C
-
688409
Acremonium chrysogenum
CoA + cephalosporin C
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
biotechnology
studies of the enzyme will be useful in gaining a full view of the possibilities for overcoming the acetyl transfer bottleneck and for the future development of cephalosporin antibiotics
Acremonium chrysogenum
Cloned(Commentary) (protein specific)
Commentary
Organism
into the pTWIN vector for expression in Escherichia coli BL21DE3 cells
Acremonium chrysogenum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
different structures are solved, SeMet-DAC-AT with acetyl-CoA to a resolution of 2.4 A, DAC-AT with acetyl-CoA to 2.2 A, DAC-AT with DAC/cephalosporin C to 2.5 A, and DAC-AT with DAC to 2.6 A resolution
Acremonium chrysogenum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + deacetylcephalosporin C
Acremonium chrysogenum
-
CoA + cephalosporin C
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
using a gravity-flow column containing chitin beads, cleavage of the chitin-binding domain and intein fusion partner is performed
Acremonium chrysogenum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + deacetylcephalosporin C
-
688409
Acremonium chrysogenum
CoA + cephalosporin C
-
-
-
?
Other publictions for EC 2.3.1.175
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688409
Lejon
The last step in cephalosporin ...
Acremonium chrysogenum
J. Mol. Biol.
377
935-944
2008
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673028
Ullan
Deacetylcephalosporin C produc ...
Acremonium chrysogenum
Chem. Biol.
14
329-339
2007
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2
1
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4
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657802
Velasco
Molecular characterization of ...
Acremonium chrysogenum
Biochem. J.
337
379-385
1999
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1
1
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657617
Gutierrez
Expression of the cefG gene is ...
Acremonium chrysogenum
Appl. Microbiol. Biotechnol.
48
606-614
1997
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1
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5
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1
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1
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657564
Martin
Expression of genes and proces ...
Acremonium chrysogenum
Antonie van Leeuwenhoek
65
227-243
1994
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658486
Mathison
Cloning, characterization, and ...
Acremonium chrysogenum
Curr. Genet.
23
33-41
1993
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1
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657711
Matsuda
Molecular cloning of acetyl co ...
Acremonium chrysogenum
Biochem. Biophys. Res. Commun.
182
995-1001
1992
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1
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3
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657712
Matsuda
Cloning and disruption of the ...
Acremonium chrysogenum
Biochem. Biophys. Res. Commun.
186
40-46
1992
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658291
Matsuyama
Purification of acetyl coenzym ...
Acremonium chrysogenum
Biosci. Biotechnol. Biochem.
56
1410-1412
1992
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2
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1
1
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1
1
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1
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659005
Gutierrez
The cefG gene of Cephalosporiu ...
Acremonium chrysogenum
J. Bacteriol.
174
3056-3064
1992
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1
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6
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659547
Scheidegger
-
Investigation of acetyl-CoA:de ...
Acremonium chrysogenum
J. Biotechnol.
3
109-117
1985
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5
2
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