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EC Tree
IUBMB Comments This enzyme catalyses the final step in the biosynthesis of cephalosporin C.
The enzyme appears in viruses and cellular organisms
Synonyms
dac acetyltransferase, dcpc-atf, deacetylcephalosporin c acetyltransferase, dac-at, deacetylcephalosporin-c acetyltransferase,
more
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acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase
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acetyl coenzyme A: deacetylcephalosporin C O-acetyltransferase
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acetyl coenzyme A:DAC acetyltransferase
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acetyl-CoA:DAC acetyltransferase
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acetyl-CoA:DAC O-acetyltransferase
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acetyl-CoA:DAC-acetyltransferase
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acetyl-CoA:deacetylcephalosporin C O-acetyltransferase
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acetyl-CoA:deacetylcephalosporin-C acetyltransferase
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DAC acetyltransferase
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deacetylcephalosporin C acetyltransferase
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deacetylcephalosporin-C acetyltransferase
DAC-AT
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deacetylcephalosporin-C acetyltransferase
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deacetylcephalosporin-C acetyltransferase
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acetyl-CoA + deacetylcephalosporin C = CoA + cephalosporin C
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acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase
This enzyme catalyses the final step in the biosynthesis of cephalosporin C.
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acetyl-CoA + deacetylcephalosporin C
CoA + cephalosporin C
additional information
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expression is regulated by methionine
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acetyl-CoA + deacetylcephalosporin C
CoA + cephalosporin C
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acetyl-CoA + deacetylcephalosporin C
CoA + cephalosporin C
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acetyl-CoA + deacetylcephalosporin C
CoA + cephalosporin C
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the enzyme catalyzes the final step in the biosynthesis of cephalosporin C
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acetyl-CoA + deacetylcephalosporin C
CoA + cephalosporin C
additional information
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expression is regulated by methionine
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acetyl-CoA + deacetylcephalosporin C
CoA + cephalosporin C
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acetyl-CoA + deacetylcephalosporin C
CoA + cephalosporin C
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acetyl-CoA + deacetylcephalosporin C
CoA + cephalosporin C
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the enzyme catalyzes the final step in the biosynthesis of cephalosporin C
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2,6-dihydroxybenzoic acid
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0.1 mM, 27% inhibition
coenzyme A
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0.1 mM, 61% inhibition
deacetoxycephalosporin C
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0.1 mM, 57% inhibition
Penicillin N
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0.1 mM, 14% inhibition
pyruvate
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0.1 mM, 37% inhibition
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0.04 - 0.29
deacetylcephalosporin C
0.1
acetyl-CoA
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0.04
deacetylcephalosporin C
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0.29
deacetylcephalosporin C
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25.8
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deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA2
30
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deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA98
6.7
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deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA64
7.2
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deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA71
additional information
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0.0000378 mg cephalosporin C per min per mg
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5 - 8
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no significant influence of pH-value on enzyme activity is measured after 20 min
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6 - 8.5
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pH 6.0: about 60% of maximal activity, pH 8.5: about 50% of maximal activity
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15 - 40
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15°C: about 45% of maximal activity, 40°C: about 75% of maximal activity
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4.3
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isoelectric focusing
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brenda
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UniProt
brenda
expression in Penicillium chrysogenum
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brenda
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brenda
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brenda
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CEFG_ACRCH
444
0
49161
Swiss-Prot
other Location (Reliability: 4 )
A0A0F4YVS3_TALEM
498
0
54895
TrEMBL
other Location (Reliability: 2 )
B8MR86_TALSN
Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006)
492
0
54072
TrEMBL
other Location (Reliability: 3 )
A0A084G0Y8_PSEDA
450
0
49409
TrEMBL
other Location (Reliability: 3 )
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49000
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1 * 49000, SDS-PAGE
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?
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SDS-PAGE resolves two protein bands with molecular weights of 27000 Da and 14000 Da
monomer
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1 * 49000, SDS-PAGE
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proteolytic modification
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DCPC-ATF is synthesized as a single-chain precursor protein and is then converted to the two constituent subunit DCPC-ATF by proteolytic processing
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different structures are solved, SeMet-DAC-AT with acetyl-CoA to a resolution of 2.4 A, DAC-AT with acetyl-CoA to 2.2 A, DAC-AT with DAC/cephalosporin C to 2.5 A, and DAC-AT with DAC to 2.6 A resolution
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using a gravity-flow column containing chitin beads, cleavage of the chitin-binding domain and intein fusion partner is performed
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constructions starting at each of the three in-frame ATG codons of the Acremonium chrysogenum cefG gene (Met1, Met46 and Met60) are expressed in Escherichia coli, obtaining proteins of 49000 Da, 44000 Da and 43000 Da, respectively. All three proteins show deacetylcephalosporin C acetyltransferase activity, separately or in combination
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expression in Penicillium chrysogenum
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expression in yeast, the leader peptide is not essential for expression of the enzyme activity
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expression of the cefG gene from the promoters of 1. the glyceraldehyde-3-phosphate dehydrogenase gene of Aspergillus nidulans, 2. the glucoamylase gene of Aspergillus niger, 3. the glutamate dehydrogenase and 4. the isopenicillin N synthase genes of Penicillium chrysogenum, leads to very high steady-state levels of cefG transcript and to increased deacetylcephalosporin-C acetyltransferase protein concentration
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into the pTWIN vector for expression in Escherichia coli BL21DE3 cells
Penicillium chrysogenum strains expressing cefD1, cefD2, cefEF, and cefG genes cloned form Acremonium chrysogenum are constructed, deacetylcephalosporin-C acetyltransferase is coded by the cefG gene
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synthesis
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production of acetylcephalosporin C by gene expression in Penicillium chrysogenum. Recombinant strains secrete significant amounts of deacetylcephalosporin C, but acetylcephalosporin C is not detected in culture broth. Even when accumulating intracellularly, acetylcephalosporin C is not found extracellularly
biotechnology
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Penicillium chrysogenum npe6 lacking isopenicillin N acyltransferase activity is an excellent host for production of different beta-lactam antibiotics
biotechnology
studies of the enzyme will be useful in gaining a full view of the possibilities for overcoming the acetyl transfer bottleneck and for the future development of cephalosporin antibiotics
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Martin, J.F.; Gutierrez, S.; Fernandez, F.J.; Velasco, J.; Fierro, F.; Marcos, A.T.; Kosalkova, K.
Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins
Antonie van Leeuwenhoek
65
227-243
1994
Acremonium chrysogenum
brenda
Gutierrez, S.; Velasco, J.; Marcos, A.T.; Fernandez, F.J.; Fierro, F.; Barredo, J.L.; Diez, B.; Martin, J.F.
Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum
Appl. Microbiol. Biotechnol.
48
606-614
1997
Acremonium chrysogenum
brenda
Matsuda, A.; Sugiura, H.; Matsuyama, K.; Matsumoto, H.; Ichikawa, S.; Komatsu, K.
Molecular cloning of acetyl coenzyme A: deacetylcephalosporin C O-acetyltransferase cDNA from Acremonium chrysogenum: sequence and expression of catalytic activity in yeast
Biochem. Biophys. Res. Commun.
182
995-1001
1992
Acremonium chrysogenum
brenda
Matsuda, A.; Sugiura, H.; Matsuyama, K.; Matsumoto, H.; Ichikawa, S.; Komatsu, K.
Cloning and disruption of the cefG gene encoding acetyl coenzyme A: deacetylcephalosporin C O-acetyltransferase from Acremonium chrysogenum
Biochem. Biophys. Res. Commun.
186
40-46
1992
Acremonium chrysogenum
brenda
Velasco, J.; Gutierrez, S.; Campoy, S.; Martin, J.F.
Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits
Biochem. J.
337
379-385
1999
Acremonium chrysogenum
brenda
Matsuyama, K.; Matsumoto, H.; Matsuda, A.; Sugiura, H.; Komatsu, K.; Ichikawa, S.
Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum
Biosci. Biotechnol. Biochem.
56
1410-1412
1992
Acremonium chrysogenum
brenda
Mathison, L.; Soliday, C.; Stepan, T.; Aldrich, T.; Rambosek, J.
Cloning, characterization, and use in strain improvement of the Cephalosporium acremonium gene cefG encoding acetyl transferase
Curr. Genet.
23
33-41
1993
Acremonium chrysogenum
brenda
Gutierrez, S.; Velasco, J.; Fernandez, F.J.; Martin, J.F.
The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase
J. Bacteriol.
174
3056-3064
1992
Acremonium chrysogenum
brenda
Scheidegger, A.; Gutzwiller, A.; Kuenzi, M.T.; Fiechter, A.; Nuesch, J.
Investigation of acetyl-CoA:deacetylcephalosporin C O-acetyltransferase of Cephalosporium acremonium
J. Biotechnol.
3
109-117
1985
Acremonium chrysogenum
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brenda
Ullan, R.V.; Campoy, S.; Casqueiro, J.; Fernandez, F.J.; Martin, J.F.
Deacetylcephalosporin C production in Penicillium chrysogenum by expression of the isopenicillin N epimerization, ring expansion, and acetylation genes
Chem. Biol.
14
329-339
2007
Acremonium chrysogenum
brenda
Lejon, S.; Ellis, J.; Valegard, K.
The last step in cephalosporin C formation revealed: crystal structures of deacetylcephalosporin C acetyltransferase from Acremonium chrysogenum in complexes with reaction intermediates
J. Mol. Biol.
377
935-944
2008
Acremonium chrysogenum (P39058), Acremonium chrysogenum
brenda
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