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Literature summary for 2.3.1.135 extracted from
- Kinetic mechanism of lecithin retinol acyl transferase (1993), Biochemistry, 32, 1257-1263.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 13-demethyl-13,14-dihydro-all-trans-retinyl trifluoroacetate | competitive with respect to dipalmitoylphosphocholine, uncompetitive with respect to all-trans-retinol; reversible inhibitor | Bos taurus |  |
| all-trans-retinyl-alpha-bromoacetate | irreversible inhibition | Bos taurus | |
| additional information | inactivated by trivalent arsenicals | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000243 | - |
all-trans-retinol | reaction with dipalmitoylphosphatidylcholine | Bos taurus | |
| 0.00138 | - |
dipalmitoylphosphatidylcholine | reaction with all-trans-retinol | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + all-trans-retinol | Bos taurus | enzyme essential for the biosynthesis of 11-cis-retinal and for dietary mobilization of vitamin A | all-trans-retinyl acyl esters + 2-acylglycerophosphocholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| solubilization of membranes and centrifugation, chromatography: mono Q column and Green 5-agarose column | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| retinal pigment epithelium | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 20 mM Tris-HCl pH 9, 2 mM dithiothreitol, 1 mM EDTA, 0.1% Triton X-100, 0.1 mg/ml dipalmitoylphosphatidylcholine, NaCl, stable for months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dipalmitoylphosphatidylcholine + all-trans-retinol | - |
Bos taurus | all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine | - |
r | |
| additional information | - |
Bos taurus | ? | - |
? | |
| phosphatidylcholine + all-trans-retinol | enzyme essential for the biosynthesis of 11-cis-retinal and for dietary mobilization of vitamin A | Bos taurus | all-trans-retinyl acyl esters + 2-acylglycerophosphocholine | - |
? | |
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Release 2023.1 (January 2023)
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