Cloned (Comment) | Organism |
---|---|
gene ALS, recombinant exxpression of N-terminally His6-tagged wild-type and mutant enzymes with a 85 amino acid N-terminal deletion eliminating the chloroplast transit peptide | Arabidopsis thaliana |
gene ALSa, DNA and amino acid sequence determination and analysis, genotyping, expression analysis | Poa annua |
gene ALSb, DNA and amino acid sequence determination and analysis, genotyping, expression analysis | Poa annua |
Protein Variants | Comment | Organism |
---|---|---|
A205F | site-directed mutagenesis, the enzyme mutation confers resistance to imidazolinone, sulfonylurea, triazolopyrimidines, sulfonylaminocarbonyl triazolinones, and pyrimidinyl(thio)benzoate herbicides | Arabidopsis thaliana |
A205F | the A205F substitution in acetolactate synthase, confirmed in a population of allotetraploid annual bluegrass, confers resistance to imidazolinone, sulfonylurea, triazolopyrimidines, sulfonylamino-carbonyl-triazolinones, and pyrimidinyl (thio) benzoate herbicides | Poa annua |
A205F | the A205F substitution in acetolactate synthase, confirmed in a population of allotetraploid annual bluegrass, confers resistance to imidazolinone, sulfonylurea, triazolopyrimidines, sulfonylaminocarbonyl triazolinones, and pyrimidinyl(thio)benzoate herbicides | Poa annua |
A205F | the mutation confers high resistance to imidazolinones, sulfonylureas, triazolopyrimidines, sulfonylamino-carbonyl-triazolinones, and pyrimidinyl (thio) benzoate herbicides | Poa annua |
A205V | site-directed mutagenesis | Arabidopsis thaliana |
A205V | the mutation confers resistance to imidazolinones, sulfonylureas, triazolopyrimidines, sulfonylamino-carbonyl-triazolinones, and pyrimidinyl (thio) benzoate herbicides | Poa annua |
W574L | site-directed mutagenesis | Arabidopsis thaliana |
W574L | the mutation confers resistance to imidazolinones, sulfonylureas, triazolopyrimidines, sulfonylamino-carbonyl-triazolinones, and pyrimidinyl (thio) benzoate herbicides | Poa annua |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bensulfuron | - |
Arabidopsis thaliana | |
bensulfuron | - |
Poa annua | |
bispyribac | - |
Arabidopsis thaliana | |
bispyribac | - |
Poa annua | |
florasulam | - |
Arabidopsis thaliana | |
florasulam | - |
Poa annua | |
flucarbazone | - |
Arabidopsis thaliana | |
flucarbazone | - |
Poa annua | |
foramsulfuron | - |
Arabidopsis thaliana | |
foramsulfuron | - |
Poa annua | |
imazamox | - |
Arabidopsis thaliana | |
imazamox | - |
Poa annua | |
imazapyr | - |
Arabidopsis thaliana | |
imazapyr | - |
Poa annua | |
imazaquin | - |
Arabidopsis thaliana | |
imazaquin | - |
Poa annua | |
mesosulfuron | - |
Arabidopsis thaliana | |
mesosulfuron | - |
Poa annua | |
metosulam | - |
Arabidopsis thaliana | |
metosulam | - |
Poa annua | |
nicosulfuron | - |
Arabidopsis thaliana | |
nicosulfuron | - |
Poa annua | |
primisulfuron | - |
Arabidopsis thaliana | |
primisulfuron | - |
Poa annua | |
propoxycarbazone | - |
Arabidopsis thaliana | |
propoxycarbazone | - |
Poa annua | |
prosulfuron | - |
Arabidopsis thaliana | |
prosulfuron | - |
Poa annua | |
pyriftalid | - |
Arabidopsis thaliana | |
pyriftalid | - |
Poa annua | |
pyrimidinyl-(thio) benzoates | - |
Arabidopsis thaliana | |
pyrimidinyl-(thio) benzoates | - |
Poa annua | |
pyrithiobac | - |
Arabidopsis thaliana | |
pyrithiobac | - |
Poa annua | |
pyroxsulam | - |
Arabidopsis thaliana | |
pyroxsulam | - |
Poa annua | |
sulfonylurea | - |
Poa annua | |
thiencarbazone | - |
Arabidopsis thaliana | |
thiencarbazone | - |
Poa annua | |
triazolopyrimidines | - |
Poa annua | |
trifloxysulfuron | - |
Arabidopsis thaliana | |
trifloxysulfuron | - |
Poa annua | |
tritosulfuron | - |
Arabidopsis thaliana | |
tritosulfuron | - |
Poa annua |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana | |
Mg2+ | required | Poa annua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | Arabidopsis thaliana | - |
2-acetolactate + CO2 | - |
? | |
2 pyruvate | Poa annua | - |
2-acetolactate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P17597 | - |
- |
Poa annua | - |
annual bluegrass | - |
Poa annua | A0A0M3TGF7 | Alsb; population of allotetraploid annual bluegrass, POAANR3, selected from golf course turf in Tennessee, USA, two homeologous forms of the ALS gene, ALSa and ALSb | - |
Poa annua | A0A0M4NDK0 | Alsa; population of allotetraploid annual bluegrass, POAANR3, selected from golf course turf in Tennessee, USA, two homeologous forms of the ALS gene, ALSa and ALSb | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Poa annua |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Poa annua | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Arabidopsis thaliana | 2-acetolactate + CO2 | - |
? | |
2 pyruvate | - |
Poa annua | 2-acetolactate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALS | - |
Arabidopsis thaliana |
ALS | - |
Poa annua |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
30 | - |
assay at | Poa annua |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Arabidopsis thaliana |
7 | - |
assay at | Poa annua |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required | Arabidopsis thaliana | |
FAD | required | Poa annua | |
thiamine diphosphate | - |
Poa annua |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000000000441 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | metosulam | |
0.000000000742 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | pyrithiobac | |
0.00000000133 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | mesosulfuron | |
0.00000000158 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | bispyribac | |
0.00000000288 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | prosulfuron | |
0.00000000314 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | primisulfuron | |
0.00000000343 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | florasulam | |
0.00000000412 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | pyroxsulam | |
0.00000000423 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | bensulfuron | |
0.0000000059 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | trifloxysulfuron | |
0.0000000075 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | foramsulfuron | |
0.00000000928 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | propoxycarbazone | |
0.0000000187 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | tritosulfuron | |
0.0000000245 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | flucarbazone | |
0.0000000248 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | thiencarbazone | |
0.0000000714 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | nicosulfuron | |
0.000000441 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | metosulam | |
0.000000742 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | pyrithiobac | |
0.00000133 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | mesosulfuron | |
0.00000155 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | imazaquin | |
0.00000158 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | bispyribac | |
0.00000288 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | prosulfuron | |
0.00000293 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | imazamox | |
0.00000314 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | primisulfuron | |
0.00000343 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | florasulam | |
0.00000412 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | pyroxsulam | |
0.00000423 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | bensulfuron | |
0.0000059 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | trifloxysulfuron | |
0.00000606 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | imazapyr | |
0.0000075 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | foramsulfuron | |
0.0000187 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | tritosulfuron | |
0.00002 | - |
wild type enzyme, at 37°C, pH not specified in the publication | Poa annua | pyriftalid | |
0.0000245 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | flucarbazone | |
0.0000248 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | thiencarbazone | |
0.0000714 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | nicosulfuron | |
0.0000928 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | propoxycarbazone | |
0.00155 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | imazaquin | |
0.00293 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | imazamox | |
0.00606 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | imazapyr | |
200 | - |
pH 7.0, 30°C, recombinant wild-type enzyme | Arabidopsis thaliana | pyriftalid |
General Information | Comment | Organism |
---|---|---|
malfunction | the A205F substitution in acetolactate synthase, confirmed in a population of allotetraploid annual bluegrass, confers resistance to imidazolinone, sulfonylurea, triazolopyrimidines, sulfonylaminocarbonyl triazolinones, and pyrimidinyl(thio)benzoate herbicides | Poa annua |