Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-valine | not inhibitory for catalytic subunit alone, inhibitory for catalytic subunit plus small subunit | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
expression of both small and catalytic subunit in Escherichia coli | - |
Renatured (Comment) | Organism |
---|---|
reconstitution of enzyme from small and catalytic subunit each overexpressed in Escherichia coli and purified. Small subunit protein stimulates catalytic subunit up to 7fold and confers upon it sensitivity to inhibition by valine | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.8 | - |
catalytic subunit, pH 7.0, 30°C | Saccharomyces cerevisiae |
49 | - |
catalytic subunit plus small subunit, pH 7.0, 30°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Saccharomyces cerevisiae | 2-acetolactate + CO2 | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
L-valine | catalytic subunit plus small subunit, pH 7.0, 30°C | Saccharomyces cerevisiae |