Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Methanosarcina acetivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contain 5.09 iron ions per polypeptide | Methanosarcina acetivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor | Methanosarcina acetivorans | - |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina acetivorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cobalt-Talon resin column chromatography | Methanosarcina acetivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor | - |
Methanosarcina acetivorans | S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Methanosarcina acetivorans |
? | x * 46300, calculated from amino acid sequence | Methanosarcina acetivorans |
Synonyms | Comment | Organism |
---|---|---|
methanogenesis marker protein 10 | - |
Methanosarcina acetivorans |
MMP10 | - |
Methanosarcina acetivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | required for activity | Methanosarcina acetivorans | |
S-adenosyl-L-methionine | - |
Methanosarcina acetivorans |