Information on EC 2.1.1.379 - [methyl coenzyme M reductase]-L-arginine C-5-methyltransferase

methanogenesis marker 10

methanogenesis marker protein 10

MMP10

mmpX

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2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine + reduced acceptor = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine + acceptor

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S-adenosyl-L-methionine:[methyl coenzyme M reductase]-L-arginine C-5-(S)-methyltransferase

The enzyme, present in methanogenic archaea, catalyses a modification of an L-arginine residue at the active site of EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase (better known as methyl-coenzyme M reductase), which catalyses the last and methane-releasing step of methanogenesis. The enzyme is a radical AdoMet (radical SAM) enzyme and contains a [4Fe-4S] cluster and a Coalpha-[alpha-(5-hydroxybenzimidazolyl)]-cobamide cofactor. The methyl group, which is derived from S-adenosyl-L-methionine, is transferred to the cob(I)amide cofactor, forming methylcob(III)amide as an intermediate carrier, before being transferred to the arginine residue.

2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor

2 S-adenosyl-L-methionine + [methyl coenzyme-M reductase subunit McrA]-L-arginine275 + reduced acceptor

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + [methyl coenzyme-M reductase subunit McrA]-(5S)-C-methyl-L-arginine275 + acceptor

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2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor

2 S-adenosyl-L-methionine + [methyl coenzyme-M reductase subunit McrA]-L-arginine275 + reduced acceptor

S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + [methyl coenzyme-M reductase subunit McrA]-(5S)-C-methyl-L-arginine275 + acceptor

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Cobalamin

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required for activity

methylcobalamin

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required

761494

S-adenosyl-L-methionine

Fe2+

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the enzyme contain 5.09 iron ions per polypeptide

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Methanosarcina acetivorans DSM 2834

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762454

Q8THG6

UniProt

malfunction

metabolism

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the enzyme plays an important role in methanogenesis

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G140A/G144A/G146A

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the mutant sustains a 15.8% loss in Arg275 methylation as compared to the wild type enzyme

H375F

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the mutant sustains a 0.5% loss in Arg275 methylation as compared to the wild type enzyme

H375F/DELTAN371

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the mutant sustains a 42.5% loss in Arg275 methylation as compared to the wild type enzyme

H375G

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the mutant sustains a 0.8% loss in Arg275 methylation as compared to the wild type enzyme

N212A/D213A

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the mutant sustains a 35% loss in Arg275 methylation as compared to the wild type enzyme

cobalt-Talon resin column chromatography

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expressed in Escherichia coli BL21(DE3) cells

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