Cloned (Comment) | Organism |
---|---|
- |
Shewanella putrefaciens |
- |
Acinetobacter sp. |
- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Shewanella putrefaciens | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Acinetobacter sp. | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Escherichia coli | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Azotobacter vinelandii | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Klebsiella aerogenes | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Dickeya chrysanthemi | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Proteus mirabilis | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Pseudomonas fluorescens | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Pseudomonas putida | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Pseudomonas stutzeri | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Pseudomonas syringae | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Acinetobacter sp. ADP1 | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | Q9AEP4 | comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Acinetobacter sp. ADP1 | Q9AEP4 | comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Azotobacter vinelandii | C1DSW3 | comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Dickeya chrysanthemi | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Escherichia coli | P36999 | comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Klebsiella aerogenes | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Proteus mirabilis | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Pseudomonas fluorescens | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Pseudomonas putida | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Pseudomonas stutzeri | A4VMZ0 | comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Pseudomonas syringae | Q48F48 | comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Shewanella putrefaciens | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Purification (Comment) | Organism |
---|---|
- |
Shewanella putrefaciens |
- |
Acinetobacter sp. |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | Shewanella putrefaciens | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | Acinetobacter sp. | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | Azotobacter vinelandii | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | Klebsiella aerogenes | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | Dickeya chrysanthemi | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | Proteus mirabilis | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | Pseudomonas fluorescens | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | Pseudomonas putida | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | Pseudomonas stutzeri | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro | Pseudomonas syringae | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine745 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | Acinetobacter sp. ADP1 | S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RlmA | ambiguous | Escherichia coli |
RlmAI | - |
Pseudomonas fluorescens |
RlmAI | - |
Klebsiella aerogenes |
RlmAI | - |
Pseudomonas putida |
RlmAI | - |
Proteus mirabilis |
RlmAI | - |
Dickeya chrysanthemi |
RlmAI | - |
Shewanella putrefaciens |
RlmAI | - |
Azotobacter vinelandii |
RlmAI | - |
Pseudomonas stutzeri |
RlmAI | - |
Pseudomonas syringae |
RlmAI | - |
Acinetobacter sp. |
RlmAI | - |
Escherichia coli |
rRNA large subunit methyltransferase I | - |
Pseudomonas fluorescens |
rRNA large subunit methyltransferase I | - |
Klebsiella aerogenes |
rRNA large subunit methyltransferase I | - |
Pseudomonas putida |
rRNA large subunit methyltransferase I | - |
Proteus mirabilis |
rRNA large subunit methyltransferase I | - |
Dickeya chrysanthemi |
rRNA large subunit methyltransferase I | - |
Shewanella putrefaciens |
rRNA large subunit methyltransferase I | - |
Azotobacter vinelandii |
rRNA large subunit methyltransferase I | - |
Pseudomonas stutzeri |
rRNA large subunit methyltransferase I | - |
Pseudomonas syringae |
rRNA large subunit methyltransferase I | - |
Acinetobacter sp. |
rRNA large subunit methyltransferase I | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas fluorescens |
37 | - |
assay at | Klebsiella aerogenes |
37 | - |
assay at | Pseudomonas putida |
37 | - |
assay at | Proteus mirabilis |
37 | - |
assay at | Dickeya chrysanthemi |
37 | - |
assay at | Shewanella putrefaciens |
37 | - |
assay at | Azotobacter vinelandii |
37 | - |
assay at | Pseudomonas stutzeri |
37 | - |
assay at | Pseudomonas syringae |
37 | - |
assay at | Acinetobacter sp. |
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas fluorescens |
7.5 | - |
assay at | Klebsiella aerogenes |
7.5 | - |
assay at | Pseudomonas putida |
7.5 | - |
assay at | Proteus mirabilis |
7.5 | - |
assay at | Dickeya chrysanthemi |
7.5 | - |
assay at | Shewanella putrefaciens |
7.5 | - |
assay at | Azotobacter vinelandii |
7.5 | - |
assay at | Pseudomonas stutzeri |
7.5 | - |
assay at | Pseudomonas syringae |
7.5 | - |
assay at | Acinetobacter sp. |
7.5 | - |
assay at | Escherichia coli |