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S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
the methylation at guanine745 is confined to Gram-negative bacteria
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
methylated guanines are located in hairpin 35, in domain II of prokaryotic 23S rRNA. RrmA possesses two regions that may be responsible for specific interactions with their target nucleic acid sequences: a putative Zn-finger domain in the N-terminus and the variable domain close to the C-terminus, which indicates that the enzyme exhibits the primary structural organization distinct from other nucleic acid MTases, despite sharing the common catalytic domain
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
methylation of the N1 position of nucleotide G745 in hairpin 35 of Escherichia coli 23S ribosomal RNA. Progressive truncation of the rRNA substrate shows that structures in stem-loops 33, 34 and 35 are required for methylation by RrmA. Multiple contacts between nucleotides in these stem-loops and RrmA are confirmed in footprinting experiments. No other RrmA contact is evident elsewhere in the rRNA. The RrmA contact sites on the rRNA are inaccessible in ribosomal particles and, consistent with this, 50S subunits or 70S ribosomes are not substrates for RrmA methylation. Methylate their target nucleotides only in the free RNA
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
-
-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
the methylation at guanine745 is confined to Gram-negative bacteria
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-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
-
-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
-
-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
-
-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
-
-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
-
-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro
-
-
?
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
SwissProt
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
SwissProt
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
UniProt
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
UniProt
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
brenda
-
SwissProt
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
SwissProt
brenda
-
SwissProt
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Isaksson, L.A.
Partial purification of ribosomal RNA(m1G)- and rRNA(m2G)-methylases from Escherichia coli and demonstration of some proteins affecting their apparent activity
Biochim. Biophys. Acta
312
122-133
1973
Escherichia coli (P36999)
brenda
Hansen, L.H.; Kirpekar, F.; Douthwaite, S.
Recognition of nucleotide G745 in 23 S ribosomal RNA by the RrmA methyltransferase
J. Mol. Biol.
310
1001-1010
2001
Escherichia coli (P36999)
brenda
Liu, M.; Douthwaite, S.
Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes gram-negative from gram-positive bacteria
Mol. Microbiol.
44
195-204
2002
Klebsiella aerogenes, Dickeya chrysanthemi, Proteus mirabilis, Pseudomonas putida, Pseudomonas fluorescens, Shewanella putrefaciens, Pseudomonas stutzeri (A4VMZ0), Azotobacter vinelandii (C1DSW3), Escherichia coli (P36999), Escherichia coli, Pseudomonas syringae (Q48F48), Acinetobacter sp. (Q9AEP4), Acinetobacter sp. ADP1 (Q9AEP4)
brenda
Bujnicki, J.M.; Blumenthal, R.M.; Rychlewski, L.
Sequence analysis and structure prediction of 23S rRNA:m1G methyltransferases reveals a conserved core augmented with a putative Zn-binding domain in the N-terminus and family-specific elaborations in the C-terminus
J. Mol. Microbiol. Biotechnol.
4
93-99
2002
Escherichia coli (P36999)
brenda
Das, K.; Acton, T.; Chiang, Y.; Shih, L.; Arnold, E.; Montelione, G.T.
Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site
Proc. Natl. Acad. Sci. USA
101
4041-4046
2004
Escherichia coli (P36999), Escherichia coli
brenda
Liu, M.; Novotny, G.W.; Douthwaite, S.
Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase
RNA
10
1713-1720
2004
Acinetobacter sp. (Q9AEP4)
brenda
Gustafsson, C.; Persson, B.C.
Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant
J. Bacteriol.
180
359-365
1998
Escherichia coli (P36999), Escherichia coli
brenda