BRENDA - Enzyme Database show
show all sequences of 2.1.1.173

Identification of Escherichia coli m2G methyltransferases: I. the ycbY gene encodes a methyltransferase specific for G2445 of the 23 S rRNA

Lesnyak, D.V.; Sergiev, P.V.; Bogdanov, A.A.; Dontsova, O.A.; J. Mol. Biol. 364, 20-25 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
YcbY protein carrying a C-terminal His6 tag is expressed in Escherichia coli
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
79000
-
x * 79000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Escherichia coli
N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P75864
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center
675411
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
recombinant YcbY protein is able to methylate 23S rRNA purified from the ycbY knock-out strain in vitro, assembled 50S subunits are not a substrate for the methylase
675411
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 79000, SDS-PAGE
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
YcbY protein carrying a C-terminal His6 tag is expressed in Escherichia coli
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
79000
-
x * 79000, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
Escherichia coli
N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center
675411
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
recombinant YcbY protein is able to methylate 23S rRNA purified from the ycbY knock-out strain in vitro, assembled 50S subunits are not a substrate for the methylase
675411
Escherichia coli
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 79000, SDS-PAGE
Escherichia coli
General Information
General Information
Commentary
Organism
malfunction
knock-out of the ycbY gene leads to loss of modification at G2445 and growth retardation. Growth competition with the parental wild-type strain leads to a gradual decrease in the knock-out strain cells proportion in the media
Escherichia coli
physiological function
it is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
knock-out of the ycbY gene leads to loss of modification at G2445 and growth retardation. Growth competition with the parental wild-type strain leads to a gradual decrease in the knock-out strain cells proportion in the media
Escherichia coli
physiological function
it is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center
Escherichia coli
Other publictions for EC 2.1.1.173
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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66
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2010
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1
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689247
Sergiev
Ribosomal RNA guanine-(N2)-met ...
Escherichia coli
Nucleic Acids Res.
35
2295-2301
2007
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675411
Lesnyak
Identification of Escherichia ...
Escherichia coli
J. Mol. Biol.
364
20-25
2006
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1
1
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1
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1
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2
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704261
Reddy
Characterization of the uup lo ...
Escherichia coli
J. Bacteriol.
182
1978-1986
2000
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