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Literature summary for 1.8.98.4 extracted from

  • Holmes, D.; Rotaru, A.; Ueki, T.; Shrestha, P.; Ferry, J.; Lovley, D.
    Electron and proton flux for carbon dioxide reduction in Methanosarcina barkeri during direct interspecies electron transfer (2018), Front. Microbiol., 9, 3109 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.98.4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ Methanosarcina barkeri
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 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
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 ?
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ Methanosarcina barkeri MS
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 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
 ?
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ Methanosarcina barkeri DSM 800
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 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
 ?
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ Methanosarcina barkeri MS DSM 800
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 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
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 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
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Methanosarcina barkeri DSM 800
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Methanosarcina barkeri MS
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Source Tissue

Source Tissue Comment Organism Textmining
cell culture coculture of Methanosarcina barkeri with Geobacter metallireducens, transcriptome analysis, overview. Out of the 3809 predicted protein-coding genes in the Methanosarcina barkeri MS genome, 1912 and 1909 genes have expression levels that are higher than the median in DIET-grown cells, respectively Methanosarcina barkeri
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+
-
 Methanosarcina barkeri 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
 ?
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+
-
 Methanosarcina barkeri MS 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
 ?
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+
-
 Methanosarcina barkeri DSM 800 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
 ?
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+
-
 Methanosarcina barkeri MS DSM 800 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
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 ?

Cofactor

Cofactor Comment Organism Structure
coenzyme F420 reduced F420 is a direct electron donor in the carbon dioxide reduction pathway and also serves as the electron donor for the proposed HdrABC-catalyzed electron bifurcation reaction in which reduced ferredoxin (also required for carbon dioxide reduction) is generated with simultaneous reduction of CoM-S-S-CoB Methanosarcina barkeri
Ferredoxin
-
 Methanosarcina barkeri

General Information

General Information Comment Organism
metabolism generalized model for electron and proton flux during hydrogen interspecies electron transfer (HIT) and direct interspecies electron transfer (DIET) with growth on ethanol as an example, overview. Electron and protons are transported by different mechanisms during DIET. Electron transfer is direct, through e-pili and other electrical contacts. Protons move by diffusion creating a positive proton pressure outside the cell. A mechanism for proton translocation into the cell is required for charge balance in the cytoplasm when cytoplasmic electron acceptors (EA) are reduced and to prevent acidification of the external space between cells. The transcriptome reflects faster growth during HIT and possible greater importance of membrane and outer-surface proteins during DIET. Model for electron and proton flux for carbon dioxide reduction to methane in Methanosarcina barkeri during DIET-based growth, overview Methanosarcina barkeri
additional information physiological studies of direct interspecies electron transfer (DIET) require defined co-cultures. Geobacter metallireducens is an environmentally relevant pure culture model for electron-donating partners for DIET because Geobacter species function as the electron-donating partner in important methanogenic environments such as anaerobic digesters and terrestrial wetlands. Studies with defined co-cultures in which Geobacter metallireducens is the electron-donating partner for DIET have suggested that c-type cytochromes and electrically conductive pili [e-pili] facilitate electron transport from Geobacter metallireducens to the electron accepting partner. However, Methanosarcina barkeri, a methanogen shown to participate in DIET, does not possess outer-surface c-type cytochromes or e-pili Methanosarcina barkeri
physiological function direct interspecies electron transfer (DIET) is important in diverse methanogenic environments. In DIET, electrically conductive pili [e-pili] and associated electron transport proteins deliver electrons to cytoplasmic electron acceptors. Protons have to be translocated into the cytoplasm for charge balance. The transcriptome of Methanosarcina barkeri grown via DIET in co-culture with Geobacter metallireducens compared with its transcriptome when grown via H2 interspecies transfer (HIT) with Pelobacter carbinolicus shows that transcripts for the F420H2 dehydrogenase (Fpo) and the heterodisulfide reductase, HdrABC, are more abundant during growth on DIET. Electrons delivered to methanophenazine in the cell membrane are transferred to Fpo (cf. EC 1.8.98.1). The external proton gradient necessary to drive the otherwise thermodynamically unfavorable reverse electron transport for Fpo-catalyzed F420 reduction is derived from protons released from Geobacter metallireducens metabolism. Reduced F420 is a direct electron donor in the carbon dioxide reduction pathway and also serves as the electron donor for the proposed HdrABC-catalyzed electron bifurcation reaction in which reduced ferredoxin (also required for carbon dioxide reduction) is generated with simultaneous reduction of CoM-S-S-CoB Methanosarcina barkeri