Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Methanosarcina barkeri | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the H2:heterodisulfide oxidoreductase multienzyme complex contains 70-80 nmol non-heme iron and acid labile sulfur per mg of protein | Methanosarcina barkeri | |
Ni | the H2:heterodisulfide oxidoreductase multienzyme complex contains 0.6 nmol Ni per mg of protein | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
MW of H2:heterodisulfide oxidoreductase multienzyme complex is 800000-1300000, gel filtration | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | Methanosarcina barkeri | energy-conserving step in methanohenic archaea | coenzyme B + coenzyme M | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
reduction of the heterodisulfide with H2 | Methanosarcina barkeri |
24 | - |
reduction of the heterodisulfide with benzylviologen | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | - |
Methanosarcina barkeri | coenzyme B + coenzyme M | - |
? | |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | energy-conserving step in methanohenic archaea | Methanosarcina barkeri | coenzyme B + coenzyme M | - |
? | |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced benzyl viologen | - |
Methanosarcina barkeri | coenzyme B + coenzyme M + benzyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | the H2:heterodisulfide oxidoreductase multienzyme complex is composed of nine polypepetides with MW 46000 Da, 39000 Da, 28000 Da, 25000 Da, 23000 Da, 21000 Da, 20000 Da, 16000 Da, 15000 Da, SDS-PAGE | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
H2:heterodisulfide oxidoreductase | - |
Methanosarcina barkeri |
More | the H2:heterodisulfide oxidoreductase multienzyme complex is composed of nine polypeptides with MW 46000 Da, 39000 Da, 28000 Da, 25000 Da, 23000 Da, 21000 Da, 20000 Da, 16000 Da, 15000 Da | Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b | the H2:heterodisulfide oxidoreductase multienzyme complex contains 3.2 nmol cytochrome b per mg protein | Methanosarcina barkeri | |
FAD | the H2:heterodisulfide oxidoreductase multienzyme complex contiams 0.6 nmol per mg of protein | Methanosarcina barkeri | |
additional information | the H2:heterodisukfide oxidoreductase complex does not mediate the reduction of coenzyme F420 with H2 nor the oxidation of reduced coenzyme F420 with the heterodisulfide | Methanosarcina barkeri |