Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BC21 | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant liver enzyme, native enzyme and selenomethionine enzyme, the latter is produced by microseeding with native enzyme, X-ray diffraction structure determination and analysis at 1.8 A resolution | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C62S | site directed mutagenesis, inactive mutant, Cys62 is involved in redox cycling of the FAD moiety | Rattus norvegicus |
C62S/C65S | site directed mutagenesis, inactive mutant, Cys62 and Cys65 are involved in redox cycling of the FAD moiety | Rattus norvegicus |
C65S | site directed mutagenesis, inactive mutant, Cys65 is involved in redox cycling of the FAD moiety | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
R-SH + O2 | Rattus norvegicus | - |
R-S-S-R + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q6IUU3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by heat treatment, ethanol precipitation, ion exchange chromatography, and gel filtration | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2 | reaction mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dithiothreitol + O2 | disulfide oxidase activity, reduction of flavin to a stable neutral semiquinone, further reduction can occur by addition of dithionite | Rattus norvegicus | dithiothreitol disulfide + H2O2 | - |
? | |
additional information | redox cycling of the FAD moiety is essential for enzyme activity | Rattus norvegicus | ? | - |
? | |
R-SH + O2 | - |
Rattus norvegicus | R-S-S-R + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure, stabilization by extensive noncovalent interactions and a network of hydrogen bonds, structure fo the dimer interface | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
ALRp | - |
Rattus norvegicus |
sulfhydryl oxidase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on, noncovalently bound to the enzyme, Cys62 and Cys65 are involved in redox cycling of the FAD moiety essential for enzyme activity | Rattus norvegicus |