Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Paracoccus denitrificans |
Protein Variants | Comment | Organism |
---|---|---|
E122A | substitution leads to an almost complete loss of NOR activity | Paracoccus denitrificans |
E122D | substitution mutant retains 83.2% of the activity of the wild-type enzyme | Paracoccus denitrificans |
E122Q | substitution mutant retains 1.3% of the activity of the wild-type enzyme | Paracoccus denitrificans |
E125A | substitution leads to an almost complete loss of NOR activity | Paracoccus denitrificans |
E125D | substitution leads to an almost complete loss of NOR activity | Paracoccus denitrificans |
E125Q | substitution mutant retains 13.4% of the activity of the wild-type enzyme | Paracoccus denitrificans |
E198A | substitution mutant retains 1.5% of the activity of the wild-type enzyme | Paracoccus denitrificans |
E202A | substitution mutant retains 39.3% of the activity of the wild-type enzyme | Paracoccus denitrificans |
E267A | substitution mutant retains 3.6% of the activity of the wild-type enzyme | Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
pseudoazurin | apparent KM of native enzyme | Paracoccus denitrificans | |
0.035 | - |
pseudoazurin | apparent KM of recombinant enzyme | Paracoccus denitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Paracoccus denitrificans | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitric oxide + reduced pseudoazurin | Paracoccus denitrificans | - |
nitrous oxide + pseudoazurin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 nitric oxide + reduced acceptor | - |
Paracoccus denitrificans | nitrous oxide + acceptor + H2O | - |
? | |
nitric oxide + reduced pseudoazurin | - |
Paracoccus denitrificans | nitrous oxide + pseudoazurin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nitric oxide reductase | - |
Paracoccus denitrificans |
NO reductase | - |
Paracoccus denitrificans |
NOR | - |
Paracoccus denitrificans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
pseudoazurin | mutant E122Q | Paracoccus denitrificans | |
1.01 | - |
pseudoazurin | mutant E198A | Paracoccus denitrificans | |
1.27 | - |
pseudoazurin | mutant E125D | Paracoccus denitrificans | |
1.3 | - |
pseudoazurin | recombinant wild type enzyme | Paracoccus denitrificans | |
2.58 | - |
pseudoazurin | mutant E267A | Paracoccus denitrificans | |
5.18 | - |
pseudoazurin | mutant E125A | Paracoccus denitrificans | |
7 | - |
pseudoazurin | mutant E122D | Paracoccus denitrificans | |
9.43 | - |
pseudoazurin | mutant E125Q | Paracoccus denitrificans | |
10.2 | - |
pseudoazurin | mutant E122A | Paracoccus denitrificans | |
15 | - |
pseudoazurin | native wild type enzyme | Paracoccus denitrificans | |
27.67 | - |
pseudoazurin | mutant E202A | Paracoccus denitrificans | |
58.52 | - |
pseudoazurin | mutant E122D | Paracoccus denitrificans | |
70.38 | - |
pseudoazurin | recombinant wild type enzyme | Paracoccus denitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Paracoccus denitrificans |