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show all sequences of 1.5.1.43

The essential role of spermidine in growth of Agrobacterium tumefaciens is determined by the 1,3-diaminopropane moiety

Kim, S.H.; Wang, Y.; Khomutov, M.; Khomutov, A.; Fuqua, C.; Michael, A.J.; ACS Chem. Biol. 11, 491-499 (2016)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens
-
carboxynorspermidine + H2O + NADP+
?
-
?
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens C58 / ATCC 33970
-
carboxynorspermidine + H2O + NADP+
?
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens
-
carboxyspermidine + H2O + NADP+
?
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens C58 / ATCC 33970
-
carboxyspermidine + H2O + NADP+
?
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
A9CG66
-
-
Agrobacterium tumefaciens C58 / ATCC 33970
A9CG66
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
741488
Agrobacterium tumefaciens
carboxynorspermidine + H2O + NADP+
?
-
-
?
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
741488
Agrobacterium tumefaciens C58 / ATCC 33970
carboxynorspermidine + H2O + NADP+
?
-
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
741488
Agrobacterium tumefaciens
carboxyspermidine + H2O + NADP+
?
-
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
741488
Agrobacterium tumefaciens C58 / ATCC 33970
carboxyspermidine + H2O + NADP+
?
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Agrobacterium tumefaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens
-
carboxynorspermidine + H2O + NADP+
?
-
?
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
Agrobacterium tumefaciens C58 / ATCC 33970
-
carboxynorspermidine + H2O + NADP+
?
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens
-
carboxyspermidine + H2O + NADP+
?
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
Agrobacterium tumefaciens C58 / ATCC 33970
-
carboxyspermidine + H2O + NADP+
?
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
741488
Agrobacterium tumefaciens
carboxynorspermidine + H2O + NADP+
?
-
-
?
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
741488
Agrobacterium tumefaciens C58 / ATCC 33970
carboxynorspermidine + H2O + NADP+
?
-
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
741488
Agrobacterium tumefaciens
carboxyspermidine + H2O + NADP+
?
-
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
741488
Agrobacterium tumefaciens C58 / ATCC 33970
carboxyspermidine + H2O + NADP+
?
-
-
?
General Information
General Information
Commentary
Organism
malfunction
the DELTACASDH (Atu4170) mutant strain accumulates putrescine and homospermidine but lacks spermidine. The DELTA4170 strain contains endogenously produced putrescine and homospermidine, but there is no growth of this strain in the absence of exogenously supplied polyamines. Homospermidine biosynthesis is induced only after spermidine depletion. The DELTACASDH strain grows well in medium with added exogenous N8-acetylspermidine
Agrobacterium tumefaciens
metabolism
when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Spermidine represses spermine biosynthesis, but when spermidine levels decrease, it is then converted by carboxyspermidine dehydrogenase and decarboxylase enzymes to spermine, which is resistant to retroconversion and constitutes a sequestered pool of protected 1,3-diaminopropane modules required for growth. Polyamine biosynthesis in Agrobacterium tumefaciens strain C58, overview
Agrobacterium tumefaciens
physiological function
when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Essential role of a terminal 1,3-diaminopropane moiety in growth of Agrobacterium tumefaciens
Agrobacterium tumefaciens
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the DELTACASDH (Atu4170) mutant strain accumulates putrescine and homospermidine but lacks spermidine. The DELTA4170 strain contains endogenously produced putrescine and homospermidine, but there is no growth of this strain in the absence of exogenously supplied polyamines. Homospermidine biosynthesis is induced only after spermidine depletion. The DELTACASDH strain grows well in medium with added exogenous N8-acetylspermidine
Agrobacterium tumefaciens
metabolism
when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Spermidine represses spermine biosynthesis, but when spermidine levels decrease, it is then converted by carboxyspermidine dehydrogenase and decarboxylase enzymes to spermine, which is resistant to retroconversion and constitutes a sequestered pool of protected 1,3-diaminopropane modules required for growth. Polyamine biosynthesis in Agrobacterium tumefaciens strain C58, overview
Agrobacterium tumefaciens
physiological function
when spermidine levels are pharmacologically decreased, synthesis of spermine from spermidine is induced via the same biosynthetic enzymes, carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase that produce spermidine from putrescine. Essential role of a terminal 1,3-diaminopropane moiety in growth of Agrobacterium tumefaciens
Agrobacterium tumefaciens
Other publictions for EC 1.5.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741488
Kim
The essential role of spermid ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
ACS Chem. Biol.
11
491-499
2016
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742792
Wang
Spermidine inversely influenc ...
Agrobacterium tumefaciens
J. Bacteriol.
198
2682-2691
2016
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719993
Hanfrey
Alternative spermidine biosynt ...
Campylobacter jejuni
J. Biol. Chem.
286
43301-43312
2011
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704662
Shaw
Evolution and multifarious hor ...
Rhizobium leguminosarum
J. Biol. Chem.
285
14711-14723
2010
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704647
Lee
An alternative polyamine biosy ...
Vibrio cholerae
J. Biol. Chem.
284
9899-9907
2009
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712623
Nakao
Purification and some properti ...
Vibrio alginolyticus, Vibrio alginolyticus ATCC 17749
J. Gen. Microbiol.
137
1737-1742
1991
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