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Literature summary for 1.4.1.9 extracted from

  • Jia, Y.Y.; Xie, Y.L.; Yang, L.L.; Shi, H.L.; Lu, Y.F.; Zhang, S.P.; Tang, C.D.; Yao, L.G.; Kan, Y.C.
    Expression of novel L-leucine dehydrogenase and high-level production of L-tert-leucine catalyzed by engineered Escherichia coli (2021), Front. Bioeng. Biotechnol., 9, 655522 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Planifilum fimeticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 NADH pH 9.5, 30°C Planifilum fimeticola
0.1
-
 L-leucine pH 9.5, 30°C Planifilum fimeticola
0.11
-
 NAD+ pH 9.5, 30°C Planifilum fimeticola
3.08
-
 trimethylpyruvate pH 9.5, 30°C Planifilum fimeticola

Organism

Organism UniProt Comment Textmining
Planifilum fimeticola
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51
-
 pH 9.5, 30°C Planifilum fimeticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH + H+
-
 Planifilum fimeticola L-2-aminobutanoate + H2O + NAD+
-
 r
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 Planifilum fimeticola L-leucine + H2O + NAD+
-
 r
amino(phenyl)acetate + H2O + NAD+
-
 Planifilum fimeticola oxo(phenyl)acetate + NH3 + NADH + H+
-
 r
L-leucine + H2O + NAD+
-
 Planifilum fimeticola 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
 r
L-tert-leucine + H2O + NAD+
-
 Planifilum fimeticola trimethylpyruvate + NH3 + NADH + H+
-
 r
L-valine + H2O + NAD+
-
 Planifilum fimeticola 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
 r
oxo(phenyl)acetate + NH3 + NADH + H+
-
 Planifilum fimeticola amino(phenyl)acetate + H2O + NAD+
-
 r
trimethylpyruvate + NH3 + NADH + H+
-
 Planifilum fimeticola L-tert-leucine + H2O + NAD+
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Planifilum fimeticola

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, no loss of activity Planifilum fimeticola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 NAD+ pH 9.5, 30°C Planifilum fimeticola
2.3
-
 L-leucine pH 9.5, 30°C Planifilum fimeticola
6.1
-
 NADH pH 9.5, 30°C Planifilum fimeticola
39.1
-
 trimethylpyruvate pH 9.5, 30°C Planifilum fimeticola

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.7
-
 trimethylpyruvate pH 9.5, 30°C Planifilum fimeticola
16.5
-
 NAD+ pH 9.5, 30°C Planifilum fimeticola
23.3
-
 L-leucine pH 9.5, 30°C Planifilum fimeticola
101
-
 NADH pH 9.5, 30°C Planifilum fimeticola