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Information on EC 1.4.1.9 - leucine dehydrogenase
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EC Tree
1.4.1.9 leucine dehydrogenaseIUBMB Comments
Also acts on isoleucine, valine, norvaline and norleucine.
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Word Map
- 1.4.1.9
- sphaericus
- l-valine
- synthesis
- l-isoleucine
-
l-2-aminobutyric
- l-tert-leucine
- intermedius
-
thermoactinomyces
- l-threonine
- analysis
-
space-time
-
3.5.1.5
- trimethylpyruvate
- alpha-ketoisocaproate
-
lysinibacillus
- drug development
- biotechnology
The enzyme appears in viruses and cellular organisms
Synonyms
leucine dehydrogenase, leudh, l-leucine dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
dehydrogenase, leucine
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L-leucine dehydrogenase
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L-leucine:NAD+ oxidoreductase, deaminating
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LeuDH
LeuDH
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH + H+
ordered bi-ter mechanism, in which NAD+ and L-Leu are bound and NH4+, 2-oxoisohexanoate, and NADH are released in that order
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L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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redox reaction
reduction
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redox reaction
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
L-leucine:NAD+ oxidoreductase (deaminating)
Also acts on isoleucine, valine, norvaline and norleucine.
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CAS REGISTRY NUMBER
COMMENTARY
9082-71-7
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-keto-beta-methylvalerate + NH3 + NADH
? + H2O + NAD+
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as active as 2-ketoisocaproate, wild-type enzyme
-
-
?
2-keto-gamma-methylthiobutanoate + NH3 + NADH
L-Met + H2O + NAD+
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15% of the activity with 2-ketoisocaproate, wild-type enzyme
-
-
?
2-ketocaproate + NH3 + NADH
L-2-aminohexanoate + H2O + NAD+
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69% of the activity with 2-ketoisocaproate
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-
?
2-ketoisovalerate + NH3 + NADH
L-Val + H2O + NAD+
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150% of the activity with 2-ketoisocaproate
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-
?
2-oxo-3,3-dimethylbutanoate + NH3 + NADH
L-2-amino-3,3-dimethylbutanoate + NAD+ + H2O
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-
-
-
?
2-oxo-4-methylselenobutyrate + NH3 + NADH
L-selenomethionine + H2O + NAD+
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-
-
?
2-oxobutanoate + NH3 + NADH
L-2-aminobutanoate + H2O + NAD+
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11% activity 2-oxobutanoate compared to 4-methyl-2-oxopentanoate
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r
2-oxopentanoate + NH3 + NADH
L-norvaline + H2O + NAD+
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25% activity with 2-oxopentanoate compared to 4-methyl-2-oxopentanoate
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-
r
2-oxophenylacetic acid + NH3 + NADH + H+
2-aminophenylacetic acid + H2O + NAD+
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-
-
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r
3,3-dimethyl-2-oxobutanoate + NH3 + NADH + H+
L-tert-Leu + H2O + NAD+
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-
-
-
r
3-methyl-2-oxobutanoate + NH3 + NADH + H+
L-valine + H2O + NAD+
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-
-
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r
3-methyl-2-oxopentanoate + NH3 + NADH
L-Ile + H2O + NAD+
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64% activity with 3-methyl-2-oxopentanoate compared to 4-methyl-2-oxopentanoate
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r
4-methyl-2-oxo-5,5,5-trifluoropentanoate + NH3 + NADH
2-amino-4-methyl-5,5,5-trifluoropentanoate + NAD+ + H2O
-
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH
L-Leu + H2O + NAD+
-
100% activity with 4-methyl-2-oxopentanoate
-
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r
4-methylthio-2-oxobutyrate + NH3 + NADH
L-Met + H2O + NAD+
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19% activity with 4-methylthio-2-oxobutyrate compared to 4-methyl-2-oxopentanoate
-
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r
alpha-keto beta-methylvalerate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
yield: 95%
-
-
?
alpha-ketocaproate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
yield: 80%
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-
?
alpha-ketoisocaproate + NH3 + NADH + H+
L-leucine + H2O + NAD+
yield: 92.5%
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-
?
alpha-ketoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
yield: 90%
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-
?
alpha-ketovalerate + NH3 + NADH + H+
L-norvaline + H2O + NAD+
yield: 92%
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?
amino(phenyl)acetate + H2O + NAD+
oxo(phenyl)acetate + NH3 + NADH + H+
-
-
-
-
r
DL-methionine + H2O + NAD+
3-methylthio-2-oxobutanoate + NH3 + NADH + H+
-
-
-
?
L-2-aminobutyrate + H2O + NAD+
2-oxobutanoate + NH3 + NADH
-
32% of the activity with L-Leu
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-
?
L-arginine + H2O + NAD+
5-[(diaminomethyl)amino]-2-oxopentanoic acid + NH3 + NADH + H+
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?
L-isoleucine + NH3 + NADH + H+
3-methyl-2-oxopentanoate + H2O + NAD+
-
-
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?
L-Leu + H2O + 3-acetylpyridine-deamino-NAD+
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-deamino-NADH
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as effective as NAD+
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-
?
L-Leu + H2O + 3-acetylpyridine-NAD+
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-NADH
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166% of the activity with NAD+
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-
?
L-Leu + H2O + 3-pyridinealdehyde-NAD+
4-methyl-2-oxopentanoate + NH3 + 3-pyridinealdehyde-NADH
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19% of the activity with NAD+
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?
L-Leu + H2O + deamino-NAD+
4-methyl-2-oxopentanoate + NH3 + deamino-NADH
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81% of the activity with NAD+
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-
?
L-Leu + H2O + thionicotinamide-NAD+
4-methyl-2-oxopentanoate + NH3 + thionicotinamide-NADH
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21% of the activity with NAD+
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?
L-leucine + H2O + NADP+
4-methyl-2-oxopentanoate + NH3 + NADPH + H+
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-
-
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r
L-lysine + H2O + NAD+
6-amino-2-oxohexanoate + NH3 + NADH + H+
-
69% of the activity with L-leucine
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-
?
L-norleucine + H2O + NAD+
? + NH3 + NADH
-
14% the activity with L-Leu, wild-type enzyme
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-
?
L-norvaline + H2O + NAD+
2-ketovalerate + NH3 + NADH
-
56% the activity with L-Leu, wild-type enzyme
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-
?
L-Phe + H2O + NAD+
phenylpyruvate + NH3 + NADH
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no activity of wild-type enzyme activity with mutant enzymes A113G, A113G/V291L
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-
?
L-S-methylcysteine + H2O + NAD+
3-methylthio-2-oxopropionate + NH3 + NADH
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19% of the activity with L-Leu
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-
?
L-valine + NH3 + NADH + H+
2-hydroxy-3-methylbutanoate + H2O + NAD+
-
-
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?
phenylglyoxylate + NH3 + NADH + H+
amino(phenyl)acetate + H2O + NAD+
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-
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?
phenylpyruvate + NH3 + NADH
L-Phe + H2O + NAD+
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15% of the activity with 2-ketoisocaproate, wild-type enzyme
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?
S-methyl-L-cysteine + H2O + NAD+
3-methylthio-2-oxopropanoate + NH3 + NADH
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19% the activity with L-Leu
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?
trimethylpyruvic acid + NADH + NH3 + H+
L-tert-leucine + NAD+ + H2O
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-
-
-
?
2-ketobutyrate + NH3 + NADH
L-2-aminobutyrate + H2O + NAD+
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47% of the activity with 2-ketoisocaproate, wild-type enzyme
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-
?
2-ketobutyrate + NH3 + NADH
L-2-aminobutyrate + H2O + NAD+
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70% of the activity with 2-ketoisocaproate
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?
2-ketobutyrate + NH3 + NADH
L-2-aminobutyrate + H2O + NAD+
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70% of the activity with 2-ketoisocaproate
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-
?
2-ketovalerate + NH3 + NADH
L-norvaline + H2O + NAD+
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86% of the activity with 2-ketoisocaproate, wild-type enzyme
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-
?
2-ketovalerate + NH3 + NADH
L-norvaline + H2O + NAD+
-
96% of the activity with 2-ketoisocaproate
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-
?
2-oxo-3-methylbutanoate + NH3 + NADH + H+
L-2-amino-3-methylbutanoate + H2O + NAD+
-
-
-
?
2-oxo-3-methylbutanoate + NH3 + NADH + H+
L-2-amino-3-methylbutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
-
?, r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
Roseibium aggregatum
-
-
-
-
?
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
-
-
-
-
r
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
-
46% activity compared to 2-oxoisocaproate
-
-
r
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
-
-
-
?
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
-
-
-
?
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
Roseibium aggregatum
-
-
-
-
?
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
-
-
-
-
?
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
Weizmannia coagulans NL01
-
-
-
-
?
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
L-tert-Leu + H2O + NAD+
-
-
-
-
?
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
L-tert-Leu + H2O + NAD+
-
-
-
?
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
L-tert-Leu + H2O + NAD+
-
-
-
?
4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+
L-methionine + H2O + NAD+
-
-
-
-
r
4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+
L-methionine + H2O + NAD+
-
-
-
-
r
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-4-methyl-2-aminopentanoate + H2O + NAD+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-4-methyl-2-aminopentanoate + H2O + NAD+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-4-methyl-2-aminopentanoate + H2O + NAD+
Roseibium aggregatum
-
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
-
-
-
r
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
32% of the activity with L-Leu
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
41% of the activity with L-Leu
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
14% of the activity with L-Leu
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
5.7% activity wit L-2-aminobutanoate compared to L-Leu
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
5.7% activity wit L-2-aminobutanoate compared to L-Leu
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
11% of the activity with L-Leu
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
19% of the activity with L-Leu
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
?
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-Ala + H2O + NAD+
2-oxopropanoate + NH3 + NADH
-
10% of the activity with L-Leu
-
-
?
L-Ala + H2O + NAD+
2-oxopropanoate + NH3 + NADH
-
3.4% activity with L-Ala compared to L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72% of the activity with L-Leu
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
73% of the activity with L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
54% of the activity with L-Leu, wild-type enzyme
-
-
?
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
58% of the activity with L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72.4% activity with L-Ile compared to L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72.4% activity with L-Ile compared to L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
88% of the activity with L-Leu
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72% of the activity with L-Leu
-
-
?
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72% of the activity with L-Leu
-
-
?
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
-
?
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
69% of the activity with L-leucine
-
-
?
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
the equilibrium favors synthesis of L-Leu
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
the enzyme may have a function in spore germination
-
?
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
the enzyme may have a function in spore germination
-
?
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
-
?
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
100% activity with L-Leu
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
100% activity with L-Leu
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r