Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activating nor inhibitory: Na+, Ca2+, Mg2+, Mn2+, Cu2+, and Zn2+ | Exiguobacterium sibiricum |
Application | Comment | Organism |
---|---|---|
synthesis | enzyme coupled with recombinant formate dehydrogenase is used to catalyze trimethylpyruvic acid through reductive amination to generate enantiopure L-tert-leucine. Using a fed-batch feeding strategy, up to 0.8 M of trimethylpyruvate is transformed to L-tert-leucine, with an average conversion rate of 81% and L-tert-leucine concentration of 65.6 g/l | Exiguobacterium sibiricum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Exiguobacterium sibiricum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.624 | - |
NADH | pH 8.5, 30°C | Exiguobacterium sibiricum | |
0.88 | - |
L-leucine | pH 8.5, 30°C | Exiguobacterium sibiricum | |
1.46 | - |
NAD+ | pH 8.5, 30°C | Exiguobacterium sibiricum | |
5.96 | - |
trimethylpyruvate | pH 8.5, 30°C | Exiguobacterium sibiricum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 86% residual activity | Exiguobacterium sibiricum | |
Fe2+ | 1 mM, 60% residual activity | Exiguobacterium sibiricum | |
Fe3+ | 1 mM, 48% residual activity | Exiguobacterium sibiricum | |
additional information | not activating nor inhibitory: Na+, Ca2+, Mg2+, Mn2+, Cu2+, and Zn2+ | Exiguobacterium sibiricum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Exiguobacterium sibiricum | B1YLR3 | - |
- |
Exiguobacterium sibiricum DSM 17290 | B1YLR3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | L-2-aminobutanoate + H2O + NAD+ | - |
? | |
2-oxobutanoate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum DSM 17290 | L-2-aminobutanoate + H2O + NAD+ | - |
? | |
4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | L-leucine + H2O + NAD+ | - |
? | |
4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum DSM 17290 | L-leucine + H2O + NAD+ | - |
? | |
L-isoleucine + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | 3-methyl-2-oxopentanoate + H2O + NAD+ | - |
? | |
L-leucine + H2O + NAD+ | - |
Exiguobacterium sibiricum | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? | |
L-leucine + H2O + NAD+ | - |
Exiguobacterium sibiricum DSM 17290 | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? | |
L-valine + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | 2-hydroxy-3-methylbutanoate + H2O + NAD+ | - |
? | |
phenylglyoxylate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | amino(phenyl)acetate + H2O + NAD+ | - |
? | |
pyruvate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | L-alanine + H2O + NAD+ | - |
? | |
pyruvate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum DSM 17290 | L-alanine + H2O + NAD+ | - |
? | |
trimethylpyruvate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum | L-tert-leucine + H2O + NAD+ | - |
? | |
trimethylpyruvate + NH3 + NADH + H+ | - |
Exiguobacterium sibiricum DSM 17290 | L-tert-leucine + H2O + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE and calculated from sequence | Exiguobacterium sibiricum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Exiguobacterium sibiricum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life 14.7 h | Exiguobacterium sibiricum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
L-leucine | pH 8.5, 30°C | Exiguobacterium sibiricum | |
8.4 | - |
NAD+ | pH 8.5, 30°C | Exiguobacterium sibiricum | |
45.4 | - |
trimethylpyruvate | pH 8.5, 30°C | Exiguobacterium sibiricum | |
76.9 | - |
NADH | pH 8.5, 30°C | Exiguobacterium sibiricum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Exiguobacterium sibiricum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
L-leucine | pH 8.5, 30°C | Exiguobacterium sibiricum | |
5.8 | - |
NAD+ | pH 8.5, 30°C | Exiguobacterium sibiricum | |
7.6 | - |
trimethylpyruvate | pH 8.5, 30°C | Exiguobacterium sibiricum | |
123.2 | - |
NADH | pH 8.5, 30°C | Exiguobacterium sibiricum |